Investigation of the protective roles of zinc oxide nanoparticles and plant growth promoting bacteria on DNA damage and methylation in tomato (Solanum lycopersicum L.) under salinity stress

Kamil Haliloglu, Arash Hosseinpour, Kağan Tolga Cinisli, Halil Ibrahim Ozturk, Guller Ozkan, Alireza Pour-Aboughadareh, Péter Poczai

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Salinity is an edaphic stress and dramatically restricts worldwide crop production. Nanomaterials and plant growth promoting bacteria (PGPBs) are currently used to alleviate of negative effects of various stresses on plant growth and development. In the present study, the protective effects of different levels of zinc oxide nanoparticles (0, 20, and 40 mgl–1) and plant growth promoting bacteria (no bacteria, Bacillus subtilis, Lactobacillus casei, Bacillus pumilus; PGPBs) on DNA damage and cytosine methylation changes were studied in the tomato (Solanum lycopersicum L.) seedling plant under salinity stress (50 mM NaCl). Coupled Restriction Enzyme Digestion-Random Amplification (CRED-RA) and Randomly Amplified Polymorphic DNA (RAPD) approaches were used to analyze changes in cytosine methylation and how genotoxic effects influences estimation and genomic stability. Salinity stress increased the polymorphism rate of RAPD. The relief effect of PGPBs and zinc oxide nanoparticles (ZnO-NPs) also reduced the adverse effects of salinity stress. The genomic template stability value significantly increased across application of PGPB and ZnO-NP treatments, especially, integrating of Lactobacillus casei and 40 mgl–1of ZnO-NPs. DNA hypo-methylation was also observed in all treatments. Taken together, the use of PGPBs and ZnO-NPs nanoparticles induced a positive anti-genotoxic effect. These could be alternative means to reduce genetic impairment in the tomato under salinity stress.
Original languageEnglish
JournalHorticulture environment and biotechnology
ISSN2211-3452
Publication statusSubmitted - 2020
MoE publication typeA1 Journal article-refereed

Projects

PLAGE: Genetic diversity, conservation and utilization of plant genetic resources

Poczai, P., Amiryousefi, A., Cseh, A., Karsai, I., Balla, K., Kiss, T., Bányai, J. & Illés, K.

01/09/201731/12/2022

Project: Research project

Cite this

Haliloglu, K., Hosseinpour, A., Cinisli, K. T., Ozturk, H. I., Ozkan, G., Pour-Aboughadareh, A., & Poczai, P. (2020). Investigation of the protective roles of zinc oxide nanoparticles and plant growth promoting bacteria on DNA damage and methylation in tomato (Solanum lycopersicum L.) under salinity stress. Manuscript submitted for publication.