Isolation of Platelet-Derived Extracellular Vesicles

M. Aatonen, S. Valkonen, A. Boing, Y. Yuana, R. Nieuwland, P. Siljander

Research output: Chapter in Book/Report/Conference proceedingChapterScientific

Abstract

Platelets participate in several physiological functions, including hemostasis, immunity, and development. Additionally, platelets play key roles in arterial thrombosis and cancer progression. Given this plethora of functions, there is a strong interest of the role of platelet-derived (extracellular) vesicles (PDEVs) as functional mediators and biomarkers. Moreover, the majority of the blood-borne EVs are thought to originate from either platelets or directly from the platelet precursor cells, the megakaryocytes, which reside in the bone marrow. To circumvent confusion, we use the term PDEVs for both platelet-derived and/or megakaryocyte-derived EVs. PDEVs can be isolated from blood or from isolated platelets after activation. In this chapter, we describe all commonly used PDEV isolation methods from blood and prepurified platelets.
Original languageEnglish
Title of host publicationExosomes and Microvesicle : Methods and Protocols, Methods in Molecular Biology
Number of pages12
Volume1545
PublisherSpringer
Publication date2017
Pages177-188
ISBN (Print)978-1-4939-6726-1
ISBN (Electronic)978-1-4939-6728-5
DOIs
Publication statusPublished - 2017
MoE publication typeB2 Book chapter

Fields of Science

  • Exosome
  • Extracellular vesicle
  • Gradient centrifugation
  • Immunobeads
  • Isolation
  • Microparticle
  • Platelet
  • Size-exclusion chromatography
  • 1182 Biochemistry, cell and molecular biology

Cite this

Aatonen, M., Valkonen, S., Boing, A., Yuana, Y., Nieuwland, R., & Siljander, P. (2017). Isolation of Platelet-Derived Extracellular Vesicles. In Exosomes and Microvesicle: Methods and Protocols, Methods in Molecular Biology (Vol. 1545, pp. 177-188). Springer . https://doi.org/10.1007/978-1-4939-6728-5_12
Aatonen, M. ; Valkonen, S. ; Boing, A. ; Yuana, Y. ; Nieuwland, R. ; Siljander, P. / Isolation of Platelet-Derived Extracellular Vesicles. Exosomes and Microvesicle: Methods and Protocols, Methods in Molecular Biology. Vol. 1545 Springer , 2017. pp. 177-188
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Aatonen, M, Valkonen, S, Boing, A, Yuana, Y, Nieuwland, R & Siljander, P 2017, Isolation of Platelet-Derived Extracellular Vesicles. in Exosomes and Microvesicle: Methods and Protocols, Methods in Molecular Biology. vol. 1545, Springer , pp. 177-188. https://doi.org/10.1007/978-1-4939-6728-5_12

Isolation of Platelet-Derived Extracellular Vesicles. / Aatonen, M.; Valkonen, S.; Boing, A.; Yuana, Y.; Nieuwland, R.; Siljander, P.

Exosomes and Microvesicle: Methods and Protocols, Methods in Molecular Biology. Vol. 1545 Springer , 2017. p. 177-188.

Research output: Chapter in Book/Report/Conference proceedingChapterScientific

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AU - Siljander, P.

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KW - Gradient centrifugation

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KW - Isolation

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KW - Size-exclusion chromatography

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Aatonen M, Valkonen S, Boing A, Yuana Y, Nieuwland R, Siljander P. Isolation of Platelet-Derived Extracellular Vesicles. In Exosomes and Microvesicle: Methods and Protocols, Methods in Molecular Biology. Vol. 1545. Springer . 2017. p. 177-188 https://doi.org/10.1007/978-1-4939-6728-5_12