KCC2 interacts with the dendritic cytoskeleton to promote spine development

Hong Li, Stanislav Khirug, Chunlin Cai, Anastasia Ludwig, Peter Blaesse, Julia Kolikova, Ramil Afzalov, Sarah Kate Coleman, Sari Lauri, Matti S. Airaksinen, Kari Keinanen, Leonard Khiroug, Mart Saarma, Kai Kaila, Claudio Rivera

Research output: Contribution to journalArticleScientificpeer-review

Abstract

The neuron-specific K-Cl cotransporter, KCC2, induces a developmental shift to render GABAergic transmission from depolarizing to hyperpolarizing. Now we demonstrate that KCC2, independently of its Cl- transport function, is a key factor in the maturation of dendritic spines. This morphogenic role of KCC2 in the development of excitatory synapses is mediated by structural interactions between KCC2 and the spine cytoskeleton. Here, the binding of KCC2 C-terminal domain to the cytoskeleton-associated protein 4.1 N may play an important role. A more general conclusion based on our data is that KCC2 acts as a synchronizing factor in the functional development of glutamatergic and GABAergic synapses in cortical neurons and networks.
Original languageEnglish
JournalNeuron
Volume56
Issue number6
Pages (from-to)1019-1033
Number of pages15
ISSN0896-6273
DOIs
Publication statusPublished - 2007
MoE publication typeA1 Journal article-refereed

Fields of Science

  • CL-COTRANSPORTER KCC2
  • PROTEIN 4.1 FAMILY
  • MOLECULAR CHARACTERIZATION
  • HIPPOCAMPAL-NEURONS
  • SYNAPTIC INHIBITION
  • SURFACE EXPRESSION
  • CORTICAL-NEURONS
  • RAT HIPPOCAMPUS
  • AMPA RECEPTOR
  • UP-REGULATION

Cite this

Li, Hong ; Khirug, Stanislav ; Cai, Chunlin ; Ludwig, Anastasia ; Blaesse, Peter ; Kolikova, Julia ; Afzalov, Ramil ; Coleman, Sarah Kate ; Lauri, Sari ; Airaksinen, Matti S. ; Keinanen, Kari ; Khiroug, Leonard ; Saarma, Mart ; Kaila, Kai ; Rivera, Claudio. / KCC2 interacts with the dendritic cytoskeleton to promote spine development. In: Neuron. 2007 ; Vol. 56, No. 6. pp. 1019-1033.
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title = "KCC2 interacts with the dendritic cytoskeleton to promote spine development",
abstract = "The neuron-specific K-Cl cotransporter, KCC2, induces a developmental shift to render GABAergic transmission from depolarizing to hyperpolarizing. Now we demonstrate that KCC2, independently of its Cl- transport function, is a key factor in the maturation of dendritic spines. This morphogenic role of KCC2 in the development of excitatory synapses is mediated by structural interactions between KCC2 and the spine cytoskeleton. Here, the binding of KCC2 C-terminal domain to the cytoskeleton-associated protein 4.1 N may play an important role. A more general conclusion based on our data is that KCC2 acts as a synchronizing factor in the functional development of glutamatergic and GABAergic synapses in cortical neurons and networks.",
keywords = "CL-COTRANSPORTER KCC2, PROTEIN 4.1 FAMILY, MOLECULAR CHARACTERIZATION, HIPPOCAMPAL-NEURONS, SYNAPTIC INHIBITION, SURFACE EXPRESSION, CORTICAL-NEURONS, RAT HIPPOCAMPUS, AMPA RECEPTOR, UP-REGULATION",
author = "Hong Li and Stanislav Khirug and Chunlin Cai and Anastasia Ludwig and Peter Blaesse and Julia Kolikova and Ramil Afzalov and Coleman, {Sarah Kate} and Sari Lauri and Airaksinen, {Matti S.} and Kari Keinanen and Leonard Khiroug and Mart Saarma and Kai Kaila and Claudio Rivera",
year = "2007",
doi = "10.1016/j.neuron.2007.10.039",
language = "English",
volume = "56",
pages = "1019--1033",
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KCC2 interacts with the dendritic cytoskeleton to promote spine development. / Li, Hong; Khirug, Stanislav; Cai, Chunlin; Ludwig, Anastasia; Blaesse, Peter; Kolikova, Julia; Afzalov, Ramil; Coleman, Sarah Kate; Lauri, Sari; Airaksinen, Matti S.; Keinanen, Kari; Khiroug, Leonard; Saarma, Mart; Kaila, Kai; Rivera, Claudio.

In: Neuron, Vol. 56, No. 6, 2007, p. 1019-1033.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - KCC2 interacts with the dendritic cytoskeleton to promote spine development

AU - Li, Hong

AU - Khirug, Stanislav

AU - Cai, Chunlin

AU - Ludwig, Anastasia

AU - Blaesse, Peter

AU - Kolikova, Julia

AU - Afzalov, Ramil

AU - Coleman, Sarah Kate

AU - Lauri, Sari

AU - Airaksinen, Matti S.

AU - Keinanen, Kari

AU - Khiroug, Leonard

AU - Saarma, Mart

AU - Kaila, Kai

AU - Rivera, Claudio

PY - 2007

Y1 - 2007

N2 - The neuron-specific K-Cl cotransporter, KCC2, induces a developmental shift to render GABAergic transmission from depolarizing to hyperpolarizing. Now we demonstrate that KCC2, independently of its Cl- transport function, is a key factor in the maturation of dendritic spines. This morphogenic role of KCC2 in the development of excitatory synapses is mediated by structural interactions between KCC2 and the spine cytoskeleton. Here, the binding of KCC2 C-terminal domain to the cytoskeleton-associated protein 4.1 N may play an important role. A more general conclusion based on our data is that KCC2 acts as a synchronizing factor in the functional development of glutamatergic and GABAergic synapses in cortical neurons and networks.

AB - The neuron-specific K-Cl cotransporter, KCC2, induces a developmental shift to render GABAergic transmission from depolarizing to hyperpolarizing. Now we demonstrate that KCC2, independently of its Cl- transport function, is a key factor in the maturation of dendritic spines. This morphogenic role of KCC2 in the development of excitatory synapses is mediated by structural interactions between KCC2 and the spine cytoskeleton. Here, the binding of KCC2 C-terminal domain to the cytoskeleton-associated protein 4.1 N may play an important role. A more general conclusion based on our data is that KCC2 acts as a synchronizing factor in the functional development of glutamatergic and GABAergic synapses in cortical neurons and networks.

KW - CL-COTRANSPORTER KCC2

KW - PROTEIN 4.1 FAMILY

KW - MOLECULAR CHARACTERIZATION

KW - HIPPOCAMPAL-NEURONS

KW - SYNAPTIC INHIBITION

KW - SURFACE EXPRESSION

KW - CORTICAL-NEURONS

KW - RAT HIPPOCAMPUS

KW - AMPA RECEPTOR

KW - UP-REGULATION

U2 - 10.1016/j.neuron.2007.10.039

DO - 10.1016/j.neuron.2007.10.039

M3 - Article

VL - 56

SP - 1019

EP - 1033

JO - Neuron

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SN - 0896-6273

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