Label-free imaging of adipogenesis by coherent anti-stokes Raman scattering microscopy

A. Isomäki, T. Sillat, M. Ainola, M. Liljestrom, Y. T. Konttinen, M. Hukkanen

Research output: Chapter in Book/Report/Conference proceedingChapterScientific

Abstract

Label-free imaging technologies to monitor the events associated with
early, intermediate and late adipogenic differentiation in multipotent
mesenchymal stromal cells (MSCs) offer an attractive and convenient
alternative to conventional fixative based lipid dyes such as Oil Red O
and Sudan Red, fluorescent labels such as LipidTOX, and more indirect
methods such as qRT-PCR analyses of specific adipocyte differentiation
markers such as peroxisome PPARγ and LPL. Coherent anti-Stokes
Raman scattering (CARS) microscopy of live cells is a sensitive and fast
imaging method enabling evaluation of the adipogenic differentiation
with chemical specificity. CARS microscopy is based on imaging
structures of interest by displaying the characteristic intrinsic vibrational
contrast of chemical bonds. The method is nontoxic, non-destructive, and
minimally invasive, thus presenting a promising method for longitudinal
analyses of live cells and tissues. CARS provides a coherently emitted
signal that is much stronger than the spontaneous Raman scattering.
The anti-Stokes signal is blue shifted from the incident wavelength,
thus reducing the non-vibrational background present in most biological
materials. In this chapter, we aim to provide a detailed approach on how
to induce adipogenic differentiation in MSC cultures, and present our
methods related to label-free CARS imaging of the events associated
with the adipogenesis.
Original languageEnglish
Title of host publicationArthritis Research
Number of pages13
Volume1142
PublisherHumana press
Publication date2014
Pages189-201
ISBN (Print)1064-3745
DOIs
Publication statusPublished - 2014
MoE publication typeB2 Book chapter

Publication series

NameMethods in Molecular Biology
Number1142
Volume2014
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Fields of Science

  • 3111 Biomedicine

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