Laccase-mediated wood surface functionalization

T Kudanga, E. N Prasetyo, Jussi Sipilä, Paula Nousiainen, P Widsten, A Kandelbauer, G. S Nyanhongo, G Guebitz

    Research output: Contribution to journalArticleScientificpeer-review

    Abstract

    Laccase-catalyzed grafting of functional molecules on wood surface presents an eco-friendly opportunity to improve wood properties. In a novel stepwise approach, functional molecules were bound to an enzymatically modified wood surface containing anchor groups. As anchor groups, phenolic amines were coupled to lignin moieties of wood using the 62 kDa Trametes hirsuta laccase. The coupling reaction to lignin was mimicked using the lignin model compounds 4-O-methyl guaiacylglycerol P-guaiacyl ether A, guaiacylglycerol beta-guaiacyl ether E, dehydrodivanillyl alcohol type dibenzodioxocin D and syringylglycerol beta-guaiacylether G. Using this approach it was possible to increase binding of the fungicides propiconazole and thiabendazole by 42% and 58%, respectively, when compared to coupling onto wood pieces which were oxidized by laccase only.
    Original languageEnglish
    JournalEngineering in Life Sciences
    Volume8
    Issue number3
    Pages (from-to)297-302
    Number of pages6
    ISSN1618-0240
    DOIs
    Publication statusPublished - 2008
    MoE publication typeA1 Journal article-refereed

    Fields of Science

    • laccase
    • lignin
    • phenolic amines
    • KRAFT PULPS
    • LIGNIN MODEL
    • ACID
    • DECOLORIZATION
    • DERIVATIVES
    • OXIDATION
    • ENZYMES

    Cite this

    Kudanga, T., Prasetyo, E. N., Sipilä, J., Nousiainen, P., Widsten, P., Kandelbauer, A., ... Guebitz, G. (2008). Laccase-mediated wood surface functionalization. Engineering in Life Sciences, 8(3), 297-302. https://doi.org/10.1002/elsc.200800011