Lipid species profiling of equine bronchoalveolar lavage cells: a comparison of lipid profiles of horses housed on two different bedding materials

Research output: Conference materialsPosterpeer-review


The effects of inflammation on equine bronchoalveolar lavage fluid (BALF) cells´ lipidome have not been studied.

The aim of this prospective crossover study was to identify lipids associated withprogression/resolution of airway inflammation. BALF cells from healthy adult horses (n=22)challenged with two bedding materials (Peat 1 – Wood shavings (WS) – Peat 2)underwent lipidomic analysis with liquid chromatography—tandem mass spectrometry. The effects of bedding period on the lipid class and species compositions were tested with rmANOVA. The individual phosphatidylcholine (PC) species correlations in the molarpercentage (mol%) data of the BALF cells were demonstrated by Principal Component Analysis (PCA).

The main results of the study were the following: Differences between the beddings were foundin the mol% levels of 68 individual lipid species. PC class and PC 32:0 levels were higherafter Peat 2 period compared to WS period (both P < .001). In the PCA, PC 32:0 (mainmolecular species 16:0_16:0) was enriched after Peat 2 whereas PC 36:4 (containing 20:4n-6,arachidonic acid) got the highest values after WS. Phosphatidylinositol (PI) 38:4 (mainmolecular species 18:0_20:4) was higher after WS compared to both peat periods (P= .004). BALF cell count correlated positively with phosphatidylserine, sphingomyelin, ceramide, hexosylceramide and triacylglycerol mol% and negativelywith PC mol% class totals. BALF cell count correlated positively with PI 38:4.

To conclude, the novel findings of this study were that equine BALF cells´ lipid species profiles indicated changes in airway inflammation between bedding materials. Furthermore, the proportions of PC 32:0 and PC 36:4 species correlated negatively, differentiating the beddings. This ratio is a promising marker of inflammation versus resolution.
Original languageEnglish
Publication statusPublished - 22 Nov 2023
MoE publication typeNot Eligible
EventEläinlääkäripäivät 2023 - Helsinki, Finland
Duration: 22 Nov 202324 Nov 2023


ConferenceEläinlääkäripäivät 2023

Fields of Science

  • 413 Veterinary science

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