Low-level colonization of methicillin-resistant Staphylococcus aureus in pigs is maintained by slowly evolving, closely related strains in Finnish pig farms

Marie Verkola, Milla Takala, Suvi Nykäsenoja, Satu Olkkola, Paula Kurittu, Saija Kiljunen, Henni Tuomala, Asko Järvinen, Annamari Heikinheimo

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Over the past two decades, livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) has become widely prevalent in pig production in Europe. The carriage status of LA-MRSA is known to vary among individual pigs, but bacterial load in pigs has rarely been studied. We assessed the quantity of LA-MRSA in nasal and skin samples of pigs and investigated the genetic diversity of the strains together with sequenced strains from national surveillance and pathology samples from the Finnish Food Authority. On two farms with assumed MRSA-positive status, farm 1 and farm 2, 10 healthy pigs were sampled three times during 2 weeks from the nares and skin (study A). On farm 1, 54 additional pigs were sampled and from confirmed MRSA-positive animals, 10 were randomly selected and transported to a clean, controlled environment for further sampling (study B). From the samples taken on farms 1 and 2 and in the controlled environment, MRSA was isolated both by direct plating and enrichment on selective media. spa types, multilocus sequence types, staphylococcal cassette chromosome mec types, resistance and virulence genes were determined. Core genome multilocus sequence typing (cgMLST) analysis was performed, including the sequences deriving from the surveillance/pathology samples from the Finnish Food Authority.
Original languageEnglish
Article number34
JournalActa Veterinaria Scandinavica
Volume64
Issue number1
Number of pages16
ISSN1751-0147
DOIs
Publication statusPublished - 2022
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 413 Veterinary science

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