Reptiles have great taxonomic diversity that is reflected in their morphology, ecology, physiology, modes of reproduction, and development. Interest in comparative and evolutionary developmental biology makes protocols for the study of reptile embryos invaluable resources. The relatively large size, seasonal breeding, and long gestation times of turtles epitomize the challenges faced by the developmental biologist. We describe protocols for the preparation of turtle embryos for ex ovo culture, electroporation, in situ hybridization, and micro-computed tomography. Because these protocols have been adapted and optimized from methods used for frog, chick, and mouse embryos, it is likely that they could be used for other reptilian species. Notes are included for alligator embryos where appropriate.
|Title of host publication||Vertebrate Embryogenesis : Embryological, Cellular and Genetic Methods|
|Publication date||Feb 2019|
|Publication status||Published - Feb 2019|
|MoE publication type||B2 Book chapter|
|Name||Methods in Molecular Biology|
Moustakas-Verho, J., McLennan, R., Spengler, J., Kulesa, P., & Cebra-Thomas, J. A. (2019). Manipulation of developmental function in turtles with notes on alligators. In Vertebrate Embryogenesis: Embryological, Cellular and Genetic Methods (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9009-2_14