Massively parallel sequencing of single cells by epicPCR links functional genes with phylogenetic markers

Sarah J Spencer; Manu V Tamminen; Sarah P Preheim; Mira T Guo; Adrian W Briggs; Ilana L Brito; David A Weitz; Leena K Pitkanen; Francois Vigneault; Marko P. Juhani Virta; Eric J Alm

doi:10.1038/ismej.2015.124

Massively parallel sequencing of single cells by epicPCR links functional genes with phylogenetic markers

Sarah J Spencer, Manu V Tamminen, Sarah P Preheim, Mira T Guo, Adrian W Briggs, Ilana L Brito, David A Weitz, Leena K Pitkanen, Francois Vigneault, Marko P. Juhani Virta, Eric J Alm

Research output: Contribution to journal › Article › Scientific › peer-review

Original language	English
Journal	ISME Journal
Volume	10
Issue number	2
Pages (from-to)	427-436
Number of pages	10
ISSN	1751-7370
DOIs	https://doi.org/10.1038/ismej.2015.124
Publication status	Published - 2016
MoE publication type	A1 Journal article-refereed

Fields of Science

1181 Ecology, evolutionary biology
1183 Plant biology, microbiology, virology
INDIVIDUAL ENVIRONMENTAL BACTERIA
SULFATE-REDUCING BACTERIA
MICROFLUIDIC DIGITAL PCR
EMULSION-PCR
SULFITE REDUCTASES
AMPLIFICATION
SEDIMENTS
ALIGNMENT
DATABASE
PRIMERS

Access to Document

10.1038/ismej.2015.124

2 Active

Single cell genomics pipeline
Lyra, C., Tamminen, M. & Pärnänen, K. M. M.
01/04/2014 → …
Project: Research project
Genomics and metagenomics of bacterial antibiotic and metal resistance
Tamminen, M., Karkman, A., Pitkänen, L., Muziasari, W., Lyra, C., Pärnänen, K. M. M., Muurinen, J., Hynninen, A. & Virta, M.
01/01/2005 → …
Project: Research project

Cite this

@article{28c9b608af27455ba9448c8032ff8599,

title = "Massively parallel sequencing of single cells by epicPCR links functional genes with phylogenetic markers",

keywords = "1181 Ecology, evolutionary biology, 1183 Plant biology, microbiology, virology, INDIVIDUAL ENVIRONMENTAL BACTERIA , SULFATE-REDUCING BACTERIA , MICROFLUIDIC DIGITAL PCR , EMULSION-PCR , SULFITE REDUCTASES , AMPLIFICATION , SEDIMENTS , ALIGNMENT , DATABASE , PRIMERS",

author = "Spencer, {Sarah J} and Tamminen, {Manu V} and Preheim, {Sarah P} and Guo, {Mira T} and Briggs, {Adrian W} and Brito, {Ilana L} and {A Weitz}, David and Pitkanen, {Leena K} and Francois Vigneault and Virta, {Marko P. Juhani} and Alm, {Eric J}",

year = "2016",

doi = "10.1038/ismej.2015.124",

language = "English",

volume = "10",

pages = "427--436",

journal = "ISME Journal",

issn = "1751-7370",

publisher = "Nature Publishing Group",

number = "2",

}

Massively parallel sequencing of single cells by epicPCR links functional genes with phylogenetic markers. / Spencer, Sarah J; Tamminen, Manu V; Preheim, Sarah P; Guo, Mira T; Briggs, Adrian W; Brito, Ilana L; A Weitz, David; Pitkanen, Leena K; Vigneault, Francois; Virta, Marko P. Juhani; Alm, Eric J.

In: ISME Journal, Vol. 10, No. 2, 2016, p. 427-436.

Research output: Contribution to journal › Article › Scientific › peer-review

TY - JOUR

T1 - Massively parallel sequencing of single cells by epicPCR links functional genes with phylogenetic markers

AU - Spencer, Sarah J

AU - Tamminen, Manu V

AU - Preheim, Sarah P

AU - Guo, Mira T

AU - Briggs, Adrian W

AU - Brito, Ilana L

AU - A Weitz, David

AU - Pitkanen, Leena K

AU - Vigneault, Francois

AU - Virta, Marko P. Juhani

AU - Alm, Eric J

PY - 2016

Y1 - 2016

KW - 1181 Ecology, evolutionary biology

KW - 1183 Plant biology, microbiology, virology

KW - INDIVIDUAL ENVIRONMENTAL BACTERIA

KW - SULFATE-REDUCING BACTERIA

KW - MICROFLUIDIC DIGITAL PCR

KW - EMULSION-PCR

KW - SULFITE REDUCTASES

KW - AMPLIFICATION

KW - SEDIMENTS

KW - ALIGNMENT

KW - DATABASE

KW - PRIMERS

U2 - 10.1038/ismej.2015.124

DO - 10.1038/ismej.2015.124

M3 - Article

VL - 10

SP - 427

EP - 436

JO - ISME Journal

JF - ISME Journal

SN - 1751-7370

IS - 2

ER -

Massively parallel sequencing of single cells by epicPCR links functional genes with phylogenetic markers

Fields of Science

Access to Document

Projects

Single cell genomics pipeline

Genomics and metagenomics of bacterial antibiotic and metal resistance

Cite this