Means to Quantify Vascular Cell File Numbers in Different Tissues

Helena E. Arents, Gugan Eswaran, Matouš Glanc, Ari Pekka Mähönen, Bert De Rybel

Research output: Chapter in Book/Report/Conference proceedingChapterScientificpeer-review

Abstract

Oriented cell divisions are crucial throughout plant development to define the final size and shape of organs and tissues. As most of the tissues in mature roots and stems are derived from vascular tissues, studying cell proliferation in the vascular cell lineage is of great importance. Although perturbations of vascular development are often visible already at the whole plant macroscopic phenotype level, a more detailed characterization of the vascular anatomy, cellular organization, and differentiation status of specific vascular cell types can provide insights into which pathway or developmental program is affected. In particular, defects in the frequency or orientation of cell divisions can be reliably identified from the number of vascular cell files. Here, we provide a detailed description of the different clearing, staining, and imaging techniques that allow precise phenotypic analysis of vascular tissues in different organs of the model plant Arabidopsis thaliana throughout development, including the quantification of cell file numbers, differentiation status of vascular cell types, and expression of reporter genes.

Original languageEnglish
Title of host publicationPlant Cell Division : Methods and Protocols
EditorsMarie-Cécile Caillaud
Number of pages25
Place of PublicationNew York
PublisherHumana press
Publication date2022
Pages155-179
ISBN (Print)978-1-0716-1743-4, 978-1-0716-1746-5
ISBN (Electronic)978-1-0716-1744-1
DOIs
Publication statusPublished - 2022
MoE publication typeA3 Book chapter

Publication series

NameMethods in Molecular Biology
Volume2382
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Fields of Science

  • Arabidopsis thaliana
  • Cambium
  • Cell division
  • Cell proliferation
  • Confocal microscopy
  • Histochemical staining
  • Light microscopy
  • Phloem
  • Procambium
  • Vascular tissues
  • Xylem
  • 11831 Plant biology

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