Meat processing plant microbiome and contamination patterns of cold-tolerant bacteria causing food safety and spoilage risks in the manufacture of vacuum-packaged, cooked sausages.

Jenni Hultman, Riitta Rahkila, Javeria Ali, Juho Rousu, K. Johanna Björkroth

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Refrigerated food processing facilities are specific man-made niches likely to harbor cold-tolerant bacteria. To characterize this type of microbiota and study the link between processing plant and product microbiomes, we followed and compared microbiota associated with the raw materials and processing stages of a vacuum-packaged, cooked sausage product affected by a prolonged quality fluctuation with occasional spoilage manifestations during shelf life. A total of 195 samples were subjected to culturing and amplicon sequence analyses. Abundant mesophilic psychrotrophs were detected within the microbiomes throughout the different compartments of the production plant environment. However, each of the main genera of food safety and quality interest, e.g., Leuconostoc, Brochothrix, and Yersinia, had their own characteristic patterns of contamination. Bacteria from the genus Leuconostoc, commonly causing spoilage of cold-stored, modified-atmosphere-packaged foods, were detected in high abundance (up to >98%) in the sausages studied. The same operational taxonomic units (OTUs) were, however, detected in lower abundances in raw meat and emulsion (average relative abundance of 2% +/- 5%), as well as on the processing plant surfaces (<4%). A completely different abundance profile was found for OTUs phylogenetically close to the species Yersinia pseudotuberculosis. These OTUs were detected in high abundance (up to 28%) on the processing plant surfaces but to a lesser extent (<1%) in raw meat, sausage emulsion, and sausages. The fact that Yersinia-like OTUs were found on the surfaces of a high-hygiene packaging compartment raises food safety concerns related to their resilient existence on surfaces.
Original languageEnglish
JournalApplied and Environmental Microbiology
Volume81
Issue number20
Pages (from-to)7088-7097
Number of pages10
ISSN0099-2240
DOIs
Publication statusPublished - Oct 2015
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 1183 Plant biology, microbiology, virology
  • LACTIC-ACID BACTERIA
  • GELIDUM SUBSP GASICOMITATUM
  • GENE RESTRICTION PATTERNS
  • LEUCONOSTOC-GELIDUM
  • INDOOR ENVIRONMENT
  • RNA
  • DIVERSITY
  • TOOL
  • COMMUNITY
  • ALIGNMENT

Cite this

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title = "Meat processing plant microbiome and contamination patterns of cold-tolerant bacteria causing food safety and spoilage risks in the manufacture of vacuum-packaged, cooked sausages.",
abstract = "Refrigerated food processing facilities are specific man-made niches likely to harbor cold-tolerant bacteria. To characterize this type of microbiota and study the link between processing plant and product microbiomes, we followed and compared microbiota associated with the raw materials and processing stages of a vacuum-packaged, cooked sausage product affected by a prolonged quality fluctuation with occasional spoilage manifestations during shelf life. A total of 195 samples were subjected to culturing and amplicon sequence analyses. Abundant mesophilic psychrotrophs were detected within the microbiomes throughout the different compartments of the production plant environment. However, each of the main genera of food safety and quality interest, e.g., Leuconostoc, Brochothrix, and Yersinia, had their own characteristic patterns of contamination. Bacteria from the genus Leuconostoc, commonly causing spoilage of cold-stored, modified-atmosphere-packaged foods, were detected in high abundance (up to >98{\%}) in the sausages studied. The same operational taxonomic units (OTUs) were, however, detected in lower abundances in raw meat and emulsion (average relative abundance of 2{\%} +/- 5{\%}), as well as on the processing plant surfaces (<4{\%}). A completely different abundance profile was found for OTUs phylogenetically close to the species Yersinia pseudotuberculosis. These OTUs were detected in high abundance (up to 28{\%}) on the processing plant surfaces but to a lesser extent (<1{\%}) in raw meat, sausage emulsion, and sausages. The fact that Yersinia-like OTUs were found on the surfaces of a high-hygiene packaging compartment raises food safety concerns related to their resilient existence on surfaces.",
keywords = "1183 Plant biology, microbiology, virology, LACTIC-ACID BACTERIA, GELIDUM SUBSP GASICOMITATUM, GENE RESTRICTION PATTERNS, LEUCONOSTOC-GELIDUM, INDOOR ENVIRONMENT, RNA, DIVERSITY, TOOL, COMMUNITY, ALIGNMENT",
author = "Jenni Hultman and Riitta Rahkila and Javeria Ali and Juho Rousu and Bj{\"o}rkroth, {K. Johanna}",
year = "2015",
month = "10",
doi = "10.1128/AEM.02228-15",
language = "English",
volume = "81",
pages = "7088--7097",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
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Meat processing plant microbiome and contamination patterns of cold-tolerant bacteria causing food safety and spoilage risks in the manufacture of vacuum-packaged, cooked sausages. / Hultman, Jenni ; Rahkila, Riitta ; Ali, Javeria; Rousu, Juho ; Björkroth, K. Johanna.

In: Applied and Environmental Microbiology, Vol. 81, No. 20, 10.2015, p. 7088-7097.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Meat processing plant microbiome and contamination patterns of cold-tolerant bacteria causing food safety and spoilage risks in the manufacture of vacuum-packaged, cooked sausages.

AU - Hultman, Jenni

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AU - Ali, Javeria

AU - Rousu, Juho

AU - Björkroth, K. Johanna

PY - 2015/10

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AB - Refrigerated food processing facilities are specific man-made niches likely to harbor cold-tolerant bacteria. To characterize this type of microbiota and study the link between processing plant and product microbiomes, we followed and compared microbiota associated with the raw materials and processing stages of a vacuum-packaged, cooked sausage product affected by a prolonged quality fluctuation with occasional spoilage manifestations during shelf life. A total of 195 samples were subjected to culturing and amplicon sequence analyses. Abundant mesophilic psychrotrophs were detected within the microbiomes throughout the different compartments of the production plant environment. However, each of the main genera of food safety and quality interest, e.g., Leuconostoc, Brochothrix, and Yersinia, had their own characteristic patterns of contamination. Bacteria from the genus Leuconostoc, commonly causing spoilage of cold-stored, modified-atmosphere-packaged foods, were detected in high abundance (up to >98%) in the sausages studied. The same operational taxonomic units (OTUs) were, however, detected in lower abundances in raw meat and emulsion (average relative abundance of 2% +/- 5%), as well as on the processing plant surfaces (<4%). A completely different abundance profile was found for OTUs phylogenetically close to the species Yersinia pseudotuberculosis. These OTUs were detected in high abundance (up to 28%) on the processing plant surfaces but to a lesser extent (<1%) in raw meat, sausage emulsion, and sausages. The fact that Yersinia-like OTUs were found on the surfaces of a high-hygiene packaging compartment raises food safety concerns related to their resilient existence on surfaces.

KW - 1183 Plant biology, microbiology, virology

KW - LACTIC-ACID BACTERIA

KW - GELIDUM SUBSP GASICOMITATUM

KW - GENE RESTRICTION PATTERNS

KW - LEUCONOSTOC-GELIDUM

KW - INDOOR ENVIRONMENT

KW - RNA

KW - DIVERSITY

KW - TOOL

KW - COMMUNITY

KW - ALIGNMENT

U2 - 10.1128/AEM.02228-15

DO - 10.1128/AEM.02228-15

M3 - Article

VL - 81

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EP - 7097

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

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