Abstract

Sympatric species are known to host the same parasites species. Nevertheless, surveys examining parasite assemblages in sympatric species are rare. To understand how parasite assemblages in sympatric host species differ in a given locality, we used a noninvasive identification method based on high-throughput sequencing. We collected fecal samples from sympatric species in Ranomafana National Park, Madagascar, from September to December in 2010, 2011, and 2012 and identified their parasites by metabarcoding, sequencing a region of the small ribosomal subunit (18S) gene. Our survey included 11 host species, including endemic primates, rodents, frogs, gastropods, and nonendemic rats and dogs. We collected 872 samples, of which 571 contained nematodes and 249 were successfully sequenced. We identified nine putative species of parasites, although their correspondence to actual parasite species is not clear as the resolution of the marker gene differs between nematode clades. For the host species that we successfully sampled with 10 or more positive occurrences of nematodes, i.e., mouse lemurs (Microcebus rufus), black rats (Rattus rattus), and frogs (Anura), the parasite assemblage compositions differed significantly among host species, sampling sites, and sampling years. Our metabarcoding method shows promise in interrogating parasite assemblages in sympatric host species and our results emphasize the importance of choosing marker regions for parasite identification accuracy.
Original languageEnglish
JournalInternational Journal of Primatology
Volume39
Issue number1
Pages (from-to)49–64
Number of pages16
ISSN0164-0291
DOIs
Publication statusPublished - Feb 2018
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 1181 Ecology, evolutionary biology
  • Invasive species
  • Lemurs
  • Metabarcoding
  • Noninvasive sampling
  • Parasites
  • ALOUATTA-PALLIATA-MEXICANA
  • NONINVASIVE ASSESSMENT
  • RAIN-FORESTS
  • DNA BARCODE
  • PARASITES
  • BIODIVERSITY
  • TRANSMISSION
  • COMMUNITIES
  • POPULATIONS
  • DIVERSITY

Cite this

@article{75fe6c8fc5264467a179d5bdb0692be3,
title = "Metabarcoding gastrointestinal nematodes in sympatric endemic and non-endemic species in Ranomafana National Park, Madagascar",
abstract = "Sympatric species are known to host the same parasites species. Nevertheless, surveys examining parasite assemblages in sympatric species are rare. To understand how parasite assemblages in sympatric host species differ in a given locality, we used a noninvasive identification method based on high-throughput sequencing. We collected fecal samples from sympatric species in Ranomafana National Park, Madagascar, from September to December in 2010, 2011, and 2012 and identified their parasites by metabarcoding, sequencing a region of the small ribosomal subunit (18S) gene. Our survey included 11 host species, including endemic primates, rodents, frogs, gastropods, and nonendemic rats and dogs. We collected 872 samples, of which 571 contained nematodes and 249 were successfully sequenced. We identified nine putative species of parasites, although their correspondence to actual parasite species is not clear as the resolution of the marker gene differs between nematode clades. For the host species that we successfully sampled with 10 or more positive occurrences of nematodes, i.e., mouse lemurs (Microcebus rufus), black rats (Rattus rattus), and frogs (Anura), the parasite assemblage compositions differed significantly among host species, sampling sites, and sampling years. Our metabarcoding method shows promise in interrogating parasite assemblages in sympatric host species and our results emphasize the importance of choosing marker regions for parasite identification accuracy.",
keywords = "1181 Ecology, evolutionary biology, Invasive species, Lemurs, Metabarcoding, Noninvasive sampling, Parasites, ALOUATTA-PALLIATA-MEXICANA, NONINVASIVE ASSESSMENT, RAIN-FORESTS, DNA BARCODE, PARASITES, BIODIVERSITY, TRANSMISSION, COMMUNITIES, POPULATIONS, DIVERSITY",
author = "Aivelo, {Tuomas Juho Eero} and Medlar, {Alan John} and L{\"o}ytynoja, {Ari Pekka} and Laakkonen, {Juha Tapio} and Jernvall, {Jukka Tapani}",
year = "2018",
month = "2",
doi = "10.1007/s10764-017-0010-x",
language = "English",
volume = "39",
pages = "49–64",
journal = "International Journal of Primatology",
issn = "0164-0291",
publisher = "SPRINGER NEW YORK LLC",
number = "1",

}

TY - JOUR

T1 - Metabarcoding gastrointestinal nematodes in sympatric endemic and non-endemic species in Ranomafana National Park, Madagascar

AU - Aivelo, Tuomas Juho Eero

AU - Medlar, Alan John

AU - Löytynoja, Ari Pekka

AU - Laakkonen, Juha Tapio

AU - Jernvall, Jukka Tapani

PY - 2018/2

Y1 - 2018/2

N2 - Sympatric species are known to host the same parasites species. Nevertheless, surveys examining parasite assemblages in sympatric species are rare. To understand how parasite assemblages in sympatric host species differ in a given locality, we used a noninvasive identification method based on high-throughput sequencing. We collected fecal samples from sympatric species in Ranomafana National Park, Madagascar, from September to December in 2010, 2011, and 2012 and identified their parasites by metabarcoding, sequencing a region of the small ribosomal subunit (18S) gene. Our survey included 11 host species, including endemic primates, rodents, frogs, gastropods, and nonendemic rats and dogs. We collected 872 samples, of which 571 contained nematodes and 249 were successfully sequenced. We identified nine putative species of parasites, although their correspondence to actual parasite species is not clear as the resolution of the marker gene differs between nematode clades. For the host species that we successfully sampled with 10 or more positive occurrences of nematodes, i.e., mouse lemurs (Microcebus rufus), black rats (Rattus rattus), and frogs (Anura), the parasite assemblage compositions differed significantly among host species, sampling sites, and sampling years. Our metabarcoding method shows promise in interrogating parasite assemblages in sympatric host species and our results emphasize the importance of choosing marker regions for parasite identification accuracy.

AB - Sympatric species are known to host the same parasites species. Nevertheless, surveys examining parasite assemblages in sympatric species are rare. To understand how parasite assemblages in sympatric host species differ in a given locality, we used a noninvasive identification method based on high-throughput sequencing. We collected fecal samples from sympatric species in Ranomafana National Park, Madagascar, from September to December in 2010, 2011, and 2012 and identified their parasites by metabarcoding, sequencing a region of the small ribosomal subunit (18S) gene. Our survey included 11 host species, including endemic primates, rodents, frogs, gastropods, and nonendemic rats and dogs. We collected 872 samples, of which 571 contained nematodes and 249 were successfully sequenced. We identified nine putative species of parasites, although their correspondence to actual parasite species is not clear as the resolution of the marker gene differs between nematode clades. For the host species that we successfully sampled with 10 or more positive occurrences of nematodes, i.e., mouse lemurs (Microcebus rufus), black rats (Rattus rattus), and frogs (Anura), the parasite assemblage compositions differed significantly among host species, sampling sites, and sampling years. Our metabarcoding method shows promise in interrogating parasite assemblages in sympatric host species and our results emphasize the importance of choosing marker regions for parasite identification accuracy.

KW - 1181 Ecology, evolutionary biology

KW - Invasive species

KW - Lemurs

KW - Metabarcoding

KW - Noninvasive sampling

KW - Parasites

KW - ALOUATTA-PALLIATA-MEXICANA

KW - NONINVASIVE ASSESSMENT

KW - RAIN-FORESTS

KW - DNA BARCODE

KW - PARASITES

KW - BIODIVERSITY

KW - TRANSMISSION

KW - COMMUNITIES

KW - POPULATIONS

KW - DIVERSITY

U2 - 10.1007/s10764-017-0010-x

DO - 10.1007/s10764-017-0010-x

M3 - Article

VL - 39

SP - 49

EP - 64

JO - International Journal of Primatology

JF - International Journal of Primatology

SN - 0164-0291

IS - 1

ER -