Metabarcoding successfully tracks temporal changes in eukaryotic communities in coastal sediments

I. S. Salonen, P.-M. Chronopoulou, E. Leskinen, K. A. Koho

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Metabarcoding is a method that combines high-throughput DNA sequencing and DNA-based identification. Previously, this method has been successfully used to target spatial variation of eukaryote communities in marine sediments, however, the temporal changes in these communities remain understudied. Here, we follow the temporal changes of the eukaryote communities in Baltic Sea surface sediments collected from two coastal localities during three seasons of two consecutive years. Our study reveals that the structure of the sediment eukaryotic ecosystem was primarily driven by annual and seasonal changes in prevailing environmental conditions, whereas spatial variation was a less significant factor in explaining the variance in eukaryotic communities over time. Therefore, our data suggests that shifts in regional climate regime or large-scale changes in the environment are the overdriving factors in shaping the coastal eukaryotic sediment ecosystems rather than small-scale changes in local environmental conditions or heterogeneity in ecosystem structure. More studies targeting temporal changes are needed to further understand the long-term trends in ecosystem stability and response to climate change. Furthermore, this work contributes to the recent efforts in developing metabarcoding applications for environmental biomonitoring, proving a comprehensive option for traditional monitoring approaches.

Original languageEnglish
Article numberfiy226
JournalFEMS Microbiology Ecology
Volume95
Issue number1
Number of pages11
ISSN0168-6496
DOIs
Publication statusPublished - Jan 2019
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 18S rRNA gene
  • BALTIC SEA
  • DIVERSITY
  • V4
  • V9 REGIONS
  • metabarcoding
  • next-generation sequencing
  • sediment
  • temporal variation
  • 1181 Ecology, evolutionary biology
  • 1183 Plant biology, microbiology, virology

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