MICROFLUIDIC IMMOBILIZED ENZYME REACTOR FOR DETERMINING THE ELIMINATION OF ENVIRONMENTAL DRUG RESIDUES IN FISH

Vera Kouhi; Tea Pihlaja; Elisa Ollikainen; Tiina Sikanen

MICROFLUIDIC IMMOBILIZED ENZYME REACTOR FOR DETERMINING THE ELIMINATION OF ENVIRONMENTAL DRUG RESIDUES IN FISH

Vera Kouhi, Tea Pihlaja, Elisa Ollikainen, Tiina Sikanen

Research output: Conference materials › Paper

Abstract

This work introduces microfluidic immobilized enzyme reactor for studying hepatic elimination of pharmaceuticals and other aquatic contaminants in fish in vitro. A new protocol is developed for biotinylation of fish liver microsomes and for their immobilization on micropillar arrays made of off-stoichiometric thiol-enes that
are functionalized with avidin. As the proof-of-concept data, xenobiotic glucuronidation on chip is demonstrated.

Original language	English
Pages	1236
Number of pages	1237
Publication status	Published - 4 Oct 2020
MoE publication type	Not Eligible
Event	The 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2020) - Online Duration: 4 Oct 2020 → 9 Oct 2020 Conference number: 24th https://microtas2020.org/

Conference

Conference	The 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2020)
Abbreviated title	MicroTAS
Period	04/10/2020 → 09/10/2020
Internet address	https://microtas2020.org/

Fields of Science

317 Pharmacy
Drug metabolism
Pharmaceuticals in the environment
MICROREACTORS
Enzyme immobilization

Cite this

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title = "MICROFLUIDIC IMMOBILIZED ENZYME REACTOR FOR DETERMINING THE ELIMINATION OF ENVIRONMENTAL DRUG RESIDUES IN FISH",

abstract = "This work introduces microfluidic immobilized enzyme reactor for studying hepatic elimination of pharmaceuticals and other aquatic contaminants in fish in vitro. A new protocol is developed for biotinylation of fish liver microsomes and for their immobilization on micropillar arrays made of off-stoichiometric thiol-enes thatare functionalized with avidin. As the proof-of-concept data, xenobiotic glucuronidation on chip is demonstrated.",

keywords = "317 Pharmacy, Drug metabolism, Pharmaceuticals in the environment, MICROREACTORS, Enzyme immobilization",

author = "Vera Kouhi and Tea Pihlaja and Elisa Ollikainen and Tiina Sikanen",

year = "2020",

month = oct,

day = "4",

language = "English",

pages = "1236",

note = "The 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2020) , MicroTAS ; Conference date: 04-10-2020 Through 09-10-2020",

url = "https://microtas2020.org/",

}

TY - CONF

T1 - MICROFLUIDIC IMMOBILIZED ENZYME REACTOR FOR DETERMINING THE ELIMINATION OF ENVIRONMENTAL DRUG RESIDUES IN FISH

AU - Kouhi, Vera

AU - Pihlaja, Tea

AU - Ollikainen, Elisa

AU - Sikanen, Tiina

N1 - Conference code: 24th

PY - 2020/10/4

Y1 - 2020/10/4

N2 - This work introduces microfluidic immobilized enzyme reactor for studying hepatic elimination of pharmaceuticals and other aquatic contaminants in fish in vitro. A new protocol is developed for biotinylation of fish liver microsomes and for their immobilization on micropillar arrays made of off-stoichiometric thiol-enes thatare functionalized with avidin. As the proof-of-concept data, xenobiotic glucuronidation on chip is demonstrated.

AB - This work introduces microfluidic immobilized enzyme reactor for studying hepatic elimination of pharmaceuticals and other aquatic contaminants in fish in vitro. A new protocol is developed for biotinylation of fish liver microsomes and for their immobilization on micropillar arrays made of off-stoichiometric thiol-enes thatare functionalized with avidin. As the proof-of-concept data, xenobiotic glucuronidation on chip is demonstrated.

KW - 317 Pharmacy

KW - Drug metabolism

KW - Pharmaceuticals in the environment

KW - MICROREACTORS

KW - Enzyme immobilization

M3 - Paper

SP - 1236

T2 - The 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2020)

Y2 - 4 October 2020 through 9 October 2020

ER -