Microsatellites for the parasitoid wasp Hyposoter horticola

Christelle Couchoux, Perttu Seppä, Saskya van Nouhuys

Research output: Contribution to journalArticleScientificpeer-review

Abstract

We developed 14 microsatellite loci for the
solitary parasitoid wasp Hyposoter horticola, an egg-larval
endoparasitoid specialist of the Glanville fritillary butterfly
Melitaea cinxia, in the A ° land islands, Finland. The microsatellites
were developed from a 454 sequencing enriched
library. The characteristics of the primer sets and
polymerase chain reactions for these microsatellite markers
are presented. Thirteen of the 14 loci tested were polymorphic
with on average 3.6 alleles per locus (range 3–6).
The expected (HE) and observed (HO) heterozygosities
ranged from 0.19 to 0.76 and 0.20 to 0.62 respectively.
These markers can be used to study population genetics
and genetic relatedness in H. horticola or related taxa, and
to assess the presence of the parasitoid in hosts.
Original languageEnglish
JournalConservation genetics resources
Volume7
Issue number2
Pages (from-to)595-597
Number of pages3
ISSN1877-7252
DOIs
Publication statusPublished - Feb 2015
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 1181 Ecology, evolutionary biology

Cite this

Couchoux, Christelle ; Seppä, Perttu ; van Nouhuys, Saskya. / Microsatellites for the parasitoid wasp Hyposoter horticola. In: Conservation genetics resources. 2015 ; Vol. 7, No. 2. pp. 595-597.
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Microsatellites for the parasitoid wasp Hyposoter horticola. / Couchoux, Christelle; Seppä, Perttu; van Nouhuys, Saskya.

In: Conservation genetics resources, Vol. 7, No. 2, 02.2015, p. 595-597.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Microsatellites for the parasitoid wasp Hyposoter horticola

AU - Couchoux, Christelle

AU - Seppä, Perttu

AU - van Nouhuys, Saskya

PY - 2015/2

Y1 - 2015/2

N2 - We developed 14 microsatellite loci for thesolitary parasitoid wasp Hyposoter horticola, an egg-larvalendoparasitoid specialist of the Glanville fritillary butterflyMelitaea cinxia, in the A ° land islands, Finland. The microsatelliteswere developed from a 454 sequencing enrichedlibrary. The characteristics of the primer sets andpolymerase chain reactions for these microsatellite markersare presented. Thirteen of the 14 loci tested were polymorphicwith on average 3.6 alleles per locus (range 3–6).The expected (HE) and observed (HO) heterozygositiesranged from 0.19 to 0.76 and 0.20 to 0.62 respectively.These markers can be used to study population geneticsand genetic relatedness in H. horticola or related taxa, andto assess the presence of the parasitoid in hosts.

AB - We developed 14 microsatellite loci for thesolitary parasitoid wasp Hyposoter horticola, an egg-larvalendoparasitoid specialist of the Glanville fritillary butterflyMelitaea cinxia, in the A ° land islands, Finland. The microsatelliteswere developed from a 454 sequencing enrichedlibrary. The characteristics of the primer sets andpolymerase chain reactions for these microsatellite markersare presented. Thirteen of the 14 loci tested were polymorphicwith on average 3.6 alleles per locus (range 3–6).The expected (HE) and observed (HO) heterozygositiesranged from 0.19 to 0.76 and 0.20 to 0.62 respectively.These markers can be used to study population geneticsand genetic relatedness in H. horticola or related taxa, andto assess the presence of the parasitoid in hosts.

KW - 1181 Ecology, evolutionary biology

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DO - 10.1007/s12686-015-0437-3

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JO - Conservation genetics resources

JF - Conservation genetics resources

SN - 1877-7252

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