Minimizing errors in RT-PCR detection and quantification of SARS-CoV-2 RNA for wastewater surveillance

Warish Ahmed; Stuart L. Simpson; Paul M. Bertsch; Kyle Bibby; Aaron Bivins; Linda L. Blackall; Silvia Bofill-Mas; Albert Bosch; Joao Brandao; Phil M. Choi; Mark Ciesielski; Erica Donner; Nishita D'Souza; Andreas H. Farnleitner; Daniel Gerrity; Raul Gonzalez; John F. Griffith; Pradip Gyawali; Charles N. Haas; Kerry A. Hamilton; Hapuarachchige Chandithal Hapuarachchi; Valerie J. Harwood; Rehnuma Haque; Greg Jackson; Stuart J. Khan; Wesaal Khan; Masaaki Kitajima; Asja Korajkic; Giuseppina La Rosa; Blythe A. Layton; Erin Lipp; Sandra L. McLellan; Brian McMinn; Gertjan Medema; Suzanne Metcalfe; Wim G. Meijer; Jochen F. Mueller; Heather Murphy; Coleen C. Naughton; Rachel T. Noble; Sudhi Payyappat; Susan Petterson; Tarja Pitkänen; Veronica B. Rajal; Brandon Reyneke; Fernando A. Roman Jr; Joan B. Rose; Marta Rusinol; Michael J. Sadowsky; Laura Sala-Comorera; Yin Xiang Setoh; Samendra P. Sherchan; Kwanrawee Sirikanchana; Wendy Smith; Joshua A. Steele; Rosalie Subburg; Erin M. Symonds; Phong Thai; Kevin Thomas; Josh Tynan; Simon Toze; Janelle Thompson; Andy S. Whiteley; Judith Chui Ching Wong; Daisuke Sano; Stefan Wuertz; Irene Xagoraraki; Qian Zhang; Amity G. Zimmer-Faust; Orin C. Shanks

doi:10.1016/j.scitotenv.2021.149877

Minimizing errors in RT-PCR detection and quantification of SARS-CoV-2 RNA for wastewater surveillance

Warish Ahmed, Stuart L. Simpson, Paul M. Bertsch, Kyle Bibby, Aaron Bivins, Linda L. Blackall, Silvia Bofill-Mas, Albert Bosch, Joao Brandao, Phil M. Choi, Mark Ciesielski, Erica Donner, Nishita D'Souza, Andreas H. Farnleitner, Daniel Gerrity, Raul Gonzalez, John F. Griffith, Pradip Gyawali, Charles N. Haas, Kerry A. HamiltonHapuarachchige Chandithal Hapuarachchi, Valerie J. Harwood, Rehnuma Haque, Greg Jackson, Stuart J. Khan, Wesaal Khan, Masaaki Kitajima, Asja Korajkic, Giuseppina La Rosa, Blythe A. Layton, Erin Lipp, Sandra L. McLellan, Brian McMinn, Gertjan Medema, Suzanne Metcalfe, Wim G. Meijer, Jochen F. Mueller, Heather Murphy, Coleen C. Naughton, Rachel T. Noble, Sudhi Payyappat, Susan Petterson, Tarja Pitkänen, Veronica B. Rajal, Brandon Reyneke, Fernando A. Roman Jr, Joan B. Rose, Marta Rusinol, Michael J. Sadowsky, Laura Sala-Comorera, Yin Xiang Setoh, Samendra P. Sherchan, Kwanrawee Sirikanchana, Wendy Smith, Joshua A. Steele, Rosalie Subburg, Erin M. Symonds, Phong Thai, Kevin Thomas, Josh Tynan, Simon Toze, Janelle Thompson, Andy S. Whiteley, Judith Chui Ching Wong, Daisuke Sano, Stefan Wuertz, Irene Xagoraraki, Qian Zhang, Amity G. Zimmer-Faust, Orin C. Shanks

Research output: Contribution to journal › Review Article › peer-review

Original language	English
Article number	149877
Journal	The Science of the Total Environment
Volume	805
Number of pages	20
ISSN	0048-9697
DOIs	https://doi.org/10.1016/j.scitotenv.2021.149877
Publication status	Published - 20 Jan 2022
MoE publication type	A2 Review article in a scientific journal

Fields of Science

COVID-19
SARS-CoV-2
Wastewater
Surveillance
False positive
False negative
RT-PCR
REAL-TIME PCR
GUIDELINES MINIMUM INFORMATION
DROPLET DIGITAL PCR
ENVIRONMENTAL SURVEILLANCE
INTERNAL CONTROLS
NUCLEIC-ACIDS
CORONAVIRUS
VIRUS
DNA
CONTAMINATION
11832 Microbiology and virology
1172 Environmental sciences

Access to Document

10.1016/j.scitotenv.2021.149877

1-s2.0-S0048969721049524-mainFinal published version, 1.97 MBLicence: CC BY-NC-ND

Cite this

Ahmed, W., Simpson, S. L., Bertsch, P. M., Bibby, K., Bivins, A., Blackall, L. L., Bofill-Mas, S., Bosch, A., Brandao, J., Choi, P. M., Ciesielski, M., Donner, E., D'Souza, N., Farnleitner, A. H., Gerrity, D., Gonzalez, R., Griffith, J. F., Gyawali, P., Haas, C. N., ... Shanks, O. C. (2022). Minimizing errors in RT-PCR detection and quantification of SARS-CoV-2 RNA for wastewater surveillance. The Science of the Total Environment, 805, Article 149877. https://doi.org/10.1016/j.scitotenv.2021.149877

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title = "Minimizing errors in RT-PCR detection and quantification of SARS-CoV-2 RNA for wastewater surveillance",

keywords = "COVID-19, SARS-CoV-2, Wastewater, Surveillance, False positive, False negative, RT-PCR, REAL-TIME PCR, GUIDELINES MINIMUM INFORMATION, DROPLET DIGITAL PCR, ENVIRONMENTAL SURVEILLANCE, INTERNAL CONTROLS, NUCLEIC-ACIDS, CORONAVIRUS, VIRUS, DNA, CONTAMINATION, 11832 Microbiology and virology, 1172 Environmental sciences",

author = "Warish Ahmed and Simpson, {Stuart L.} and Bertsch, {Paul M.} and Kyle Bibby and Aaron Bivins and Blackall, {Linda L.} and Silvia Bofill-Mas and Albert Bosch and Joao Brandao and Choi, {Phil M.} and Mark Ciesielski and Erica Donner and Nishita D'Souza and Farnleitner, {Andreas H.} and Daniel Gerrity and Raul Gonzalez and Griffith, {John F.} and Pradip Gyawali and Haas, {Charles N.} and Hamilton, {Kerry A.} and Hapuarachchi, {Hapuarachchige Chandithal} and Harwood, {Valerie J.} and Rehnuma Haque and Greg Jackson and Khan, {Stuart J.} and Wesaal Khan and Masaaki Kitajima and Asja Korajkic and {La Rosa}, Giuseppina and Layton, {Blythe A.} and Erin Lipp and McLellan, {Sandra L.} and Brian McMinn and Gertjan Medema and Suzanne Metcalfe and Meijer, {Wim G.} and Mueller, {Jochen F.} and Heather Murphy and Naughton, {Coleen C.} and Noble, {Rachel T.} and Sudhi Payyappat and Susan Petterson and Tarja Pitk{\"a}nen and Rajal, {Veronica B.} and Brandon Reyneke and Jr, {Fernando A. Roman} and Rose, {Joan B.} and Marta Rusinol and Sadowsky, {Michael J.} and Laura Sala-Comorera and Setoh, {Yin Xiang} and Sherchan, {Samendra P.} and Kwanrawee Sirikanchana and Wendy Smith and Steele, {Joshua A.} and Rosalie Subburg and Symonds, {Erin M.} and Phong Thai and Kevin Thomas and Josh Tynan and Simon Toze and Janelle Thompson and Whiteley, {Andy S.} and Wong, {Judith Chui Ching} and Daisuke Sano and Stefan Wuertz and Irene Xagoraraki and Qian Zhang and Zimmer-Faust, {Amity G.} and Shanks, {Orin C.}",

year = "2022",

month = jan,

day = "20",

doi = "10.1016/j.scitotenv.2021.149877",

language = "English",

volume = "805",

journal = "The Science of the Total Environment",

issn = "0048-9697",

publisher = "Elsevier Scientific Publ. Co",

}

Ahmed, W, Simpson, SL, Bertsch, PM, Bibby, K, Bivins, A, Blackall, LL, Bofill-Mas, S, Bosch, A, Brandao, J, Choi, PM, Ciesielski, M, Donner, E, D'Souza, N, Farnleitner, AH, Gerrity, D, Gonzalez, R, Griffith, JF, Gyawali, P, Haas, CN, Hamilton, KA, Hapuarachchi, HC, Harwood, VJ, Haque, R, Jackson, G, Khan, SJ, Khan, W, Kitajima, M, Korajkic, A, La Rosa, G, Layton, BA, Lipp, E, McLellan, SL, McMinn, B, Medema, G, Metcalfe, S, Meijer, WG, Mueller, JF, Murphy, H, Naughton, CC, Noble, RT, Payyappat, S, Petterson, S, Pitkänen, T, Rajal, VB, Reyneke, B, Jr, FAR, Rose, JB, Rusinol, M, Sadowsky, MJ, Sala-Comorera, L, Setoh, YX, Sherchan, SP, Sirikanchana, K, Smith, W, Steele, JA, Subburg, R, Symonds, EM, Thai, P, Thomas, K, Tynan, J, Toze, S, Thompson, J, Whiteley, AS, Wong, JCC, Sano, D, Wuertz, S, Xagoraraki, I, Zhang, Q, Zimmer-Faust, AG & Shanks, OC 2022, 'Minimizing errors in RT-PCR detection and quantification of SARS-CoV-2 RNA for wastewater surveillance', The Science of the Total Environment, vol. 805, 149877. https://doi.org/10.1016/j.scitotenv.2021.149877

TY - JOUR

T1 - Minimizing errors in RT-PCR detection and quantification of SARS-CoV-2 RNA for wastewater surveillance

AU - Ahmed, Warish

AU - Simpson, Stuart L.

AU - Bertsch, Paul M.

AU - Bibby, Kyle

AU - Bivins, Aaron

AU - Blackall, Linda L.

AU - Bofill-Mas, Silvia

AU - Bosch, Albert

AU - Brandao, Joao

AU - Choi, Phil M.

AU - Ciesielski, Mark

AU - Donner, Erica

AU - D'Souza, Nishita

AU - Farnleitner, Andreas H.

AU - Gerrity, Daniel

AU - Gonzalez, Raul

AU - Griffith, John F.

AU - Gyawali, Pradip

AU - Haas, Charles N.

AU - Hamilton, Kerry A.

AU - Hapuarachchi, Hapuarachchige Chandithal

AU - Harwood, Valerie J.

AU - Haque, Rehnuma

AU - Jackson, Greg

AU - Khan, Stuart J.

AU - Khan, Wesaal

AU - Kitajima, Masaaki

AU - Korajkic, Asja

AU - La Rosa, Giuseppina

AU - Layton, Blythe A.

AU - Lipp, Erin

AU - McLellan, Sandra L.

AU - McMinn, Brian

AU - Medema, Gertjan

AU - Metcalfe, Suzanne

AU - Meijer, Wim G.

AU - Mueller, Jochen F.

AU - Murphy, Heather

AU - Naughton, Coleen C.

AU - Noble, Rachel T.

AU - Payyappat, Sudhi

AU - Petterson, Susan

AU - Pitkänen, Tarja

AU - Rajal, Veronica B.

AU - Reyneke, Brandon

AU - Jr, Fernando A. Roman

AU - Rose, Joan B.

AU - Rusinol, Marta

AU - Sadowsky, Michael J.

AU - Sala-Comorera, Laura

AU - Setoh, Yin Xiang

AU - Sherchan, Samendra P.

AU - Sirikanchana, Kwanrawee

AU - Smith, Wendy

AU - Steele, Joshua A.

AU - Subburg, Rosalie

AU - Symonds, Erin M.

AU - Thai, Phong

AU - Thomas, Kevin

AU - Tynan, Josh

AU - Toze, Simon

AU - Thompson, Janelle

AU - Whiteley, Andy S.

AU - Wong, Judith Chui Ching

AU - Sano, Daisuke

AU - Wuertz, Stefan

AU - Xagoraraki, Irene

AU - Zhang, Qian

AU - Zimmer-Faust, Amity G.

AU - Shanks, Orin C.

PY - 2022/1/20

Y1 - 2022/1/20

KW - COVID-19

KW - SARS-CoV-2

KW - Wastewater

KW - Surveillance

KW - False positive

KW - False negative

KW - RT-PCR

KW - REAL-TIME PCR

KW - GUIDELINES MINIMUM INFORMATION

KW - DROPLET DIGITAL PCR

KW - ENVIRONMENTAL SURVEILLANCE

KW - INTERNAL CONTROLS

KW - NUCLEIC-ACIDS

KW - CORONAVIRUS

KW - VIRUS

KW - DNA

KW - CONTAMINATION

KW - 11832 Microbiology and virology

KW - 1172 Environmental sciences

U2 - 10.1016/j.scitotenv.2021.149877

DO - 10.1016/j.scitotenv.2021.149877

M3 - Review Article

SN - 0048-9697

VL - 805

JO - The Science of the Total Environment

JF - The Science of the Total Environment

M1 - 149877

ER -