Modelling of Beta Cell Pathophysiology Using Stem Cell-Derived Islets

Tom Barsby; Hossam Montaser; Väinö Lithovius; Hazem Ibrahim; Eliisa Vähäkangas; Sachin Muralidharan; Vikash Chandra; Jonna Saarimäki-Vire; Timo Otonkoski

doi:10.1007/978-3-031-41943-0_24

Modelling of Beta Cell Pathophysiology Using Stem Cell-Derived Islets

Tom Barsby, Hossam Montaser, Väinö Lithovius, Hazem Ibrahim, Eliisa Vähäkangas, Sachin Muralidharan, Vikash Chandra, Jonna Saarimäki-Vire, Timo Otonkoski

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Scientific › peer-review

Abstract

Diabetes mellitus is a family of metabolic disorders that arise from the loss or dysfunction of insulin secreting pancreatic beta cells. Many forms of early onset diabetes are caused by monogenic mutations in genes with critical roles in pancreatic development and endocrine cell function. Stem cell-derived islets (SC-islets) represent a powerful tool by which to dissect the mechanisms of human beta-cell loss and dysfunction derived from these causative mutations in a reproducible in vitro model. While still in its relative infancy, SC-islet modelling has successfully recapitulated many elements of monogenic pathologies in mutations related to neonatal diabetes, maturity onset diabetes of the young (MODY), and even polygenic and multi-tissue models of type 1 diabetes. In this chapter, we outline the progress that has been made in applying SC-islet technologies to multiple forms of monogenic diabetes, as well as discuss the limitations and possibilities of SC-islets in comparison to other contemporary beta-cell modelling systems.

Original language	English
Title of host publication	Pluripotent Stem Cell Therapy for Diabetes, First Edition
Editors	Lorenzo Piemonti, Jon Odorico, Timothy J . Kieffer, Valeria Sordi, Eelco de Koning
Number of pages	26
Publisher	Springer International Publishing
Publication date	1 Jan 2024
Edition	1st ed.
Pages	573-598
ISBN (Print)	978-3-031-41942-3
ISBN (Electronic)	978-3-031-41943-0
DOIs	https://doi.org/10.1007/978-3-031-41943-0_24
Publication status	Published - 1 Jan 2024
MoE publication type	A3 Book chapter

Bibliographical note

Publisher Copyright:
© The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2023.

Fields of Science

Diabetes
MODY
Monogenic diabetes
PNDM
Stem cell-derived islets
Stem cells
3111 Biomedicine

Access to Document

10.1007/978-3-031-41943-0_24

Cite this

Barsby, T., Montaser, H., Lithovius, V., Ibrahim, H., Vähäkangas, E., Muralidharan, S., Chandra, V., Saarimäki-Vire, J., & Otonkoski, T. (2024). Modelling of Beta Cell Pathophysiology Using Stem Cell-Derived Islets. In L. Piemonti, J. Odorico, T. J. . Kieffer, V. Sordi, & E. de Koning (Eds.), Pluripotent Stem Cell Therapy for Diabetes, First Edition (1st ed. ed., pp. 573-598). Springer International Publishing . https://doi.org/10.1007/978-3-031-41943-0_24

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Barsby, T, Montaser, H, Lithovius, V , Ibrahim, H , Vähäkangas, E , Muralidharan, S , Chandra, V , Saarimäki-Vire, J & Otonkoski, T 2024, Modelling of Beta Cell Pathophysiology Using Stem Cell-Derived Islets. in L Piemonti, J Odorico, TJ Kieffer, V Sordi & E de Koning (eds), Pluripotent Stem Cell Therapy for Diabetes, First Edition. 1st ed. edn, Springer International Publishing , pp. 573-598. https://doi.org/10.1007/978-3-031-41943-0_24

Modelling of Beta Cell Pathophysiology Using Stem Cell-Derived Islets. / Barsby, Tom; Montaser, Hossam; Lithovius, Väinö et al.
Pluripotent Stem Cell Therapy for Diabetes, First Edition. ed. / Lorenzo Piemonti; Jon Odorico; Timothy J . Kieffer; Valeria Sordi; Eelco de Koning. 1st ed. ed. Springer International Publishing , 2024. p. 573-598.

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Scientific › peer-review

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AU - Barsby, Tom

AU - Montaser, Hossam

AU - Lithovius, Väinö

AU - Ibrahim, Hazem

AU - Vähäkangas, Eliisa

AU - Muralidharan, Sachin

AU - Chandra, Vikash

AU - Saarimäki-Vire, Jonna

AU - Otonkoski, Timo

N1 - Publisher Copyright: © The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2023.

PY - 2024/1/1

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N2 - Diabetes mellitus is a family of metabolic disorders that arise from the loss or dysfunction of insulin secreting pancreatic beta cells. Many forms of early onset diabetes are caused by monogenic mutations in genes with critical roles in pancreatic development and endocrine cell function. Stem cell-derived islets (SC-islets) represent a powerful tool by which to dissect the mechanisms of human beta-cell loss and dysfunction derived from these causative mutations in a reproducible in vitro model. While still in its relative infancy, SC-islet modelling has successfully recapitulated many elements of monogenic pathologies in mutations related to neonatal diabetes, maturity onset diabetes of the young (MODY), and even polygenic and multi-tissue models of type 1 diabetes. In this chapter, we outline the progress that has been made in applying SC-islet technologies to multiple forms of monogenic diabetes, as well as discuss the limitations and possibilities of SC-islets in comparison to other contemporary beta-cell modelling systems.

AB - Diabetes mellitus is a family of metabolic disorders that arise from the loss or dysfunction of insulin secreting pancreatic beta cells. Many forms of early onset diabetes are caused by monogenic mutations in genes with critical roles in pancreatic development and endocrine cell function. Stem cell-derived islets (SC-islets) represent a powerful tool by which to dissect the mechanisms of human beta-cell loss and dysfunction derived from these causative mutations in a reproducible in vitro model. While still in its relative infancy, SC-islet modelling has successfully recapitulated many elements of monogenic pathologies in mutations related to neonatal diabetes, maturity onset diabetes of the young (MODY), and even polygenic and multi-tissue models of type 1 diabetes. In this chapter, we outline the progress that has been made in applying SC-islet technologies to multiple forms of monogenic diabetes, as well as discuss the limitations and possibilities of SC-islets in comparison to other contemporary beta-cell modelling systems.

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BT - Pluripotent Stem Cell Therapy for Diabetes, First Edition

A2 - Piemonti, Lorenzo

A2 - Odorico, Jon

A2 - Kieffer, Timothy J .

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PB - Springer International Publishing

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Barsby T, Montaser H, Lithovius V , Ibrahim H , Vähäkangas E , Muralidharan S et al. Modelling of Beta Cell Pathophysiology Using Stem Cell-Derived Islets. In Piemonti L, Odorico J, Kieffer TJ, Sordi V, de Koning E, editors, Pluripotent Stem Cell Therapy for Diabetes, First Edition. 1st ed. ed. Springer International Publishing . 2024. p. 573-598 doi: 10.1007/978-3-031-41943-0_24