Molecular diversity and phytochemical variability in the Iranian poppy (Papaver bracteatum Lindl.): A baseline for conservation and utilization in future breeding programmes

Ardashir Qaderi, Mansour Omidi, Alireza Pour-Aboughadareh, Péter Poczai, Javad Shaghaghi, Ali MehrAfarin, Majid Ghorbani Nohooji, Alireza Etminan

Research output: Contribution to journalArticleScientificpeer-review

Abstract

In the present investigation, 72 accessions of the Iranian poppy (Papaver bracteatum Lindl.) were analyzed for genetic diversity and population structure using start codon targeted polymorphism (SCoT) and inter simple sequence repeat (ISSR) markers along with four important phytochemical traits to provide baseline knowledge for the Iranian poppy’s breeding and conservation plans. Twelve ISSR and thirteen SCoT primers generated a total of 98 and 186 fragments with a mean of 8.17 and 14.31 fragments per primer, respectively. Polymorphic information content for ISSR and SCoT primers ranged from 0.39 to 0.45 and 0.28 to 0.34, with the resolving power ranging from 21.61 to 3.97 and 13.08 to 28.02, respectively. Neighbour-joining (NJ) based clustering grouped 72 accessions into three main groups based on two markers studied (ISSR and SCoT) and the combined data (ISSR + SCoT), which associated with their eco-geographical regions. Population structure based analysis divided 72 accessions into 3 subpopulations using ISSR markers, when SCoT was used eight subpopulations were observed. However, when the combined data was used only three subpopulations were found, which corresponded to the grouping observed with the NJ method and these results were supported by principal coordinate analyses (PCoA). Phytochemical analysis revealed that plant capsule has higher total amounts of the alkaloids; thebaine, morphine and oripavine than stem tissues. Interestingly, for the geographical parameters, latitude showed a significant and positive correlation with thebaine extracted from both stem and capsules and the regression results confirmed these associations. Taken together, our results indicated that three populations Ploor, Eil-Teymoor and Anjomane due to their high contents of alkaloids like thebaine as well as the Taham population due to its high content of morphine and oripavine have a strong enough potency to be used in the pharmacy industry.
Original languageEnglish
JournalIndustrial Crops and Products
Volume130
Pages (from-to)237-247
Number of pages11
ISSN0926-6690
DOIs
Publication statusPublished - Apr 2019
MoE publication typeA1 Journal article-refereed

Fields of Science

  • AFLP
  • ALKALOIDS
  • CODON TARGETED SCOT
  • DNA
  • GENETIC DIVERSITY
  • Gene-targeted markers
  • Genetic diversity
  • Germplasm
  • ISSR POLYMORPHISM
  • MARKERS
  • Morphine
  • POPULATION-STRUCTURE
  • Poppy
  • Population structure
  • SOMNIFERUM
  • Thebaine
  • 1181 Ecology, evolutionary biology
  • 4111 Agronomy

Cite this

Qaderi, Ardashir ; Omidi, Mansour ; Pour-Aboughadareh, Alireza ; Poczai, Péter ; Shaghaghi, Javad ; MehrAfarin, Ali ; Nohooji, Majid Ghorbani ; Etminan, Alireza . / Molecular diversity and phytochemical variability in the Iranian poppy (Papaver bracteatum Lindl.): A baseline for conservation and utilization in future breeding programmes. In: Industrial Crops and Products. 2019 ; Vol. 130. pp. 237-247.
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abstract = "In the present investigation, 72 accessions of the Iranian poppy (Papaver bracteatum Lindl.) were analyzed for genetic diversity and population structure using start codon targeted polymorphism (SCoT) and inter simple sequence repeat (ISSR) markers along with four important phytochemical traits to provide baseline knowledge for the Iranian poppy’s breeding and conservation plans. Twelve ISSR and thirteen SCoT primers generated a total of 98 and 186 fragments with a mean of 8.17 and 14.31 fragments per primer, respectively. Polymorphic information content for ISSR and SCoT primers ranged from 0.39 to 0.45 and 0.28 to 0.34, with the resolving power ranging from 21.61 to 3.97 and 13.08 to 28.02, respectively. Neighbour-joining (NJ) based clustering grouped 72 accessions into three main groups based on two markers studied (ISSR and SCoT) and the combined data (ISSR + SCoT), which associated with their eco-geographical regions. Population structure based analysis divided 72 accessions into 3 subpopulations using ISSR markers, when SCoT was used eight subpopulations were observed. However, when the combined data was used only three subpopulations were found, which corresponded to the grouping observed with the NJ method and these results were supported by principal coordinate analyses (PCoA). Phytochemical analysis revealed that plant capsule has higher total amounts of the alkaloids; thebaine, morphine and oripavine than stem tissues. Interestingly, for the geographical parameters, latitude showed a significant and positive correlation with thebaine extracted from both stem and capsules and the regression results confirmed these associations. Taken together, our results indicated that three populations Ploor, Eil-Teymoor and Anjomane due to their high contents of alkaloids like thebaine as well as the Taham population due to its high content of morphine and oripavine have a strong enough potency to be used in the pharmacy industry.",
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author = "Ardashir Qaderi and Mansour Omidi and Alireza Pour-Aboughadareh and P{\'e}ter Poczai and Javad Shaghaghi and Ali MehrAfarin and Nohooji, {Majid Ghorbani} and Alireza Etminan",
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Molecular diversity and phytochemical variability in the Iranian poppy (Papaver bracteatum Lindl.): A baseline for conservation and utilization in future breeding programmes. / Qaderi, Ardashir ; Omidi, Mansour ; Pour-Aboughadareh, Alireza; Poczai, Péter; Shaghaghi, Javad ; MehrAfarin, Ali ; Nohooji, Majid Ghorbani; Etminan, Alireza .

In: Industrial Crops and Products, Vol. 130, 04.2019, p. 237-247.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Molecular diversity and phytochemical variability in the Iranian poppy (Papaver bracteatum Lindl.): A baseline for conservation and utilization in future breeding programmes

AU - Qaderi, Ardashir

AU - Omidi, Mansour

AU - Pour-Aboughadareh, Alireza

AU - Poczai, Péter

AU - Shaghaghi, Javad

AU - MehrAfarin, Ali

AU - Nohooji, Majid Ghorbani

AU - Etminan, Alireza

PY - 2019/4

Y1 - 2019/4

N2 - In the present investigation, 72 accessions of the Iranian poppy (Papaver bracteatum Lindl.) were analyzed for genetic diversity and population structure using start codon targeted polymorphism (SCoT) and inter simple sequence repeat (ISSR) markers along with four important phytochemical traits to provide baseline knowledge for the Iranian poppy’s breeding and conservation plans. Twelve ISSR and thirteen SCoT primers generated a total of 98 and 186 fragments with a mean of 8.17 and 14.31 fragments per primer, respectively. Polymorphic information content for ISSR and SCoT primers ranged from 0.39 to 0.45 and 0.28 to 0.34, with the resolving power ranging from 21.61 to 3.97 and 13.08 to 28.02, respectively. Neighbour-joining (NJ) based clustering grouped 72 accessions into three main groups based on two markers studied (ISSR and SCoT) and the combined data (ISSR + SCoT), which associated with their eco-geographical regions. Population structure based analysis divided 72 accessions into 3 subpopulations using ISSR markers, when SCoT was used eight subpopulations were observed. However, when the combined data was used only three subpopulations were found, which corresponded to the grouping observed with the NJ method and these results were supported by principal coordinate analyses (PCoA). Phytochemical analysis revealed that plant capsule has higher total amounts of the alkaloids; thebaine, morphine and oripavine than stem tissues. Interestingly, for the geographical parameters, latitude showed a significant and positive correlation with thebaine extracted from both stem and capsules and the regression results confirmed these associations. Taken together, our results indicated that three populations Ploor, Eil-Teymoor and Anjomane due to their high contents of alkaloids like thebaine as well as the Taham population due to its high content of morphine and oripavine have a strong enough potency to be used in the pharmacy industry.

AB - In the present investigation, 72 accessions of the Iranian poppy (Papaver bracteatum Lindl.) were analyzed for genetic diversity and population structure using start codon targeted polymorphism (SCoT) and inter simple sequence repeat (ISSR) markers along with four important phytochemical traits to provide baseline knowledge for the Iranian poppy’s breeding and conservation plans. Twelve ISSR and thirteen SCoT primers generated a total of 98 and 186 fragments with a mean of 8.17 and 14.31 fragments per primer, respectively. Polymorphic information content for ISSR and SCoT primers ranged from 0.39 to 0.45 and 0.28 to 0.34, with the resolving power ranging from 21.61 to 3.97 and 13.08 to 28.02, respectively. Neighbour-joining (NJ) based clustering grouped 72 accessions into three main groups based on two markers studied (ISSR and SCoT) and the combined data (ISSR + SCoT), which associated with their eco-geographical regions. Population structure based analysis divided 72 accessions into 3 subpopulations using ISSR markers, when SCoT was used eight subpopulations were observed. However, when the combined data was used only three subpopulations were found, which corresponded to the grouping observed with the NJ method and these results were supported by principal coordinate analyses (PCoA). Phytochemical analysis revealed that plant capsule has higher total amounts of the alkaloids; thebaine, morphine and oripavine than stem tissues. Interestingly, for the geographical parameters, latitude showed a significant and positive correlation with thebaine extracted from both stem and capsules and the regression results confirmed these associations. Taken together, our results indicated that three populations Ploor, Eil-Teymoor and Anjomane due to their high contents of alkaloids like thebaine as well as the Taham population due to its high content of morphine and oripavine have a strong enough potency to be used in the pharmacy industry.

KW - AFLP

KW - ALKALOIDS

KW - CODON TARGETED SCOT

KW - DNA

KW - GENETIC DIVERSITY

KW - Gene-targeted markers

KW - Genetic diversity

KW - Germplasm

KW - ISSR POLYMORPHISM

KW - MARKERS

KW - Morphine

KW - POPULATION-STRUCTURE

KW - Poppy

KW - Population structure

KW - SOMNIFERUM

KW - Thebaine

KW - 1181 Ecology, evolutionary biology

KW - 4111 Agronomy

U2 - 10.1016/j.indcrop.2018.12.079

DO - 10.1016/j.indcrop.2018.12.079

M3 - Article

VL - 130

SP - 237

EP - 247

JO - Industrial Crops and Products

JF - Industrial Crops and Products

SN - 0926-6690

ER -