Activities per year
Abstract
Electron microscopy remains one of the most accurate methods for the detection of autophagy and quantification of autophagic accumulation. Compared to fluorescence microscopy, the resolution of transmission electron microscopy is superior. In this chapter we describe the fine structure of early and late autophagic compartments in mammalian cells. Instructions are given for the preparation of samples for conventional electron microscopy using three different protocols suitable for cultured cells and animal tissues. We also introduce tomography as a tool. to study the three-dimensional morphology of autophagic organelles and show the morphology of a phagophore as an example. Finally, we describe a protocol for the quantification of autophagic compartments by electron microscopy and point counting.
Original language | English |
---|---|
Title of host publication | Methods in enzymology |
Number of pages | 22 |
Volume | 452 |
Publisher | Academic Press |
Publication date | 2009 |
Pages | 143-164 |
DOIs | |
Publication status | Published - 2009 |
MoE publication type | A3 Book chapter |
Fields of Science
- 118 Biological sciences
Activities
- 1 Academic visit at UH
-
Jenny Kahlhofer
Eeva-Liisa Eskelinen (Host) & Katri Anneli Kallio (Host)
1 Feb 2017 → 28 Feb 2017Activity: Hosting a visitor types › Academic visit at UH