Multistaged Nanovaccines based on Porous Silicon@Acetalated Dextran@Cancer Cell Membrane for Cancer Immunotherapy

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Abstract

In recent years, biomaterials have gained importance as vehicles and adjuvants in the formulation of novel cancer vaccines. Among the different biomaterials proposed porous silicon (PSi) provides a versatile biocompatible platform for various biomedical applications. The aim of this work was to develop a multistage nanovaccine constructed from biomaterials and a biological cancer cell membrane (CCM), and to assess its immunostimulative properties. Glass-capillary microfluidic nanoprecipitation technique was used to produce the initial two layers of a nanovaccine. TOPSi nanoparticles were encapsulated into acetalated dextran (AcDEX) or sperminemodified AcDEX (SpAcDEX) and further co-extruded together with vesicles derived from CCM to obtain the final core-shell system (TOPSi@AcDEX@CCM), in order to combine the antigenic composition of tumor lysate with the adjuvant properties of the PSi nanoparticles chosen. TOPSi@SpAcDEX particles were further functionalized with a model antigen, Trp2, to provide the final system, TOPSi@SpAcDEX-Trp2, for targeting. The nanovaccines presented high monodispersity and cytocompatibility,
induced expression of co-stimulatory signals in both immortal cell lines and in
peripheral blood monocytes, and enhanced the secretion of IFN-γ. Overall, the
developed nanovaccines showed promising adjuvant properties and the possibility of encapsulating the nanosystems with materials derived from the patient's tumor cells opens new prospectives for personalized cancer therapy.
Original languageEnglish
Article number1603239
JournalAdvanced Materials
Volume29
Issue number7
Number of pages9
ISSN0935-9648
DOIs
Publication statusPublished - 13 Feb 2017
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 116 Chemical sciences
  • 221 Nano-technology
  • 317 Pharmacy
  • DELIVERY PLATFORM
  • DRUG-DELIVERY
  • SYNTHETIC NANOPARTICLES
  • ANTITUMOR IMMUNITY
  • CROSS-PRESENTATION
  • SURFACE-CHEMISTRY
  • DENDRITIC CELLS
  • MICROPARTICLES
  • VACCINES
  • SIZE

Cite this

@article{4575d3f84d154e43a3c05378765c1e72,
title = "Multistaged Nanovaccines based on Porous Silicon@Acetalated Dextran@Cancer Cell Membrane for Cancer Immunotherapy",
abstract = "In recent years, biomaterials have gained importance as vehicles and adjuvants in the formulation of novel cancer vaccines. Among the different biomaterials proposed porous silicon (PSi) provides a versatile biocompatible platform for various biomedical applications. The aim of this work was to develop a multistage nanovaccine constructed from biomaterials and a biological cancer cell membrane (CCM), and to assess its immunostimulative properties. Glass-capillary microfluidic nanoprecipitation technique was used to produce the initial two layers of a nanovaccine. TOPSi nanoparticles were encapsulated into acetalated dextran (AcDEX) or sperminemodified AcDEX (SpAcDEX) and further co-extruded together with vesicles derived from CCM to obtain the final core-shell system (TOPSi@AcDEX@CCM), in order to combine the antigenic composition of tumor lysate with the adjuvant properties of the PSi nanoparticles chosen. TOPSi@SpAcDEX particles were further functionalized with a model antigen, Trp2, to provide the final system, TOPSi@SpAcDEX-Trp2, for targeting. The nanovaccines presented high monodispersity and cytocompatibility,induced expression of co-stimulatory signals in both immortal cell lines and inperipheral blood monocytes, and enhanced the secretion of IFN-γ. Overall, thedeveloped nanovaccines showed promising adjuvant properties and the possibility of encapsulating the nanosystems with materials derived from the patient's tumor cells opens new prospectives for personalized cancer therapy.",
keywords = "116 Chemical sciences, 221 Nano-technology, 317 Pharmacy, DELIVERY PLATFORM , DRUG-DELIVERY , SYNTHETIC NANOPARTICLES , ANTITUMOR IMMUNITY , CROSS-PRESENTATION , SURFACE-CHEMISTRY , DENDRITIC CELLS , MICROPARTICLES , VACCINES , SIZE",
author = "Flavia Fontana and Mohammad-Ali Shahbazi and Dongfei Liu and Hongbo Zhang and Ermei M{\"a}kil{\"a} and Jarno Salonen and Hirvonen, {Jouni Tapio} and {Almeida Santos}, Helder",
year = "2017",
month = "2",
day = "13",
doi = "10.1002/adma.201603239",
language = "English",
volume = "29",
journal = "Advanced Materials",
issn = "0935-9648",
publisher = "Wiley-VCH",
number = "7",

}

TY - JOUR

T1 - Multistaged Nanovaccines based on Porous Silicon@Acetalated Dextran@Cancer Cell Membrane for Cancer Immunotherapy

AU - Fontana, Flavia

AU - Shahbazi, Mohammad-Ali

AU - Liu, Dongfei

AU - Zhang, Hongbo

AU - Mäkilä, Ermei

AU - Salonen, Jarno

AU - Hirvonen, Jouni Tapio

AU - Almeida Santos, Helder

PY - 2017/2/13

Y1 - 2017/2/13

N2 - In recent years, biomaterials have gained importance as vehicles and adjuvants in the formulation of novel cancer vaccines. Among the different biomaterials proposed porous silicon (PSi) provides a versatile biocompatible platform for various biomedical applications. The aim of this work was to develop a multistage nanovaccine constructed from biomaterials and a biological cancer cell membrane (CCM), and to assess its immunostimulative properties. Glass-capillary microfluidic nanoprecipitation technique was used to produce the initial two layers of a nanovaccine. TOPSi nanoparticles were encapsulated into acetalated dextran (AcDEX) or sperminemodified AcDEX (SpAcDEX) and further co-extruded together with vesicles derived from CCM to obtain the final core-shell system (TOPSi@AcDEX@CCM), in order to combine the antigenic composition of tumor lysate with the adjuvant properties of the PSi nanoparticles chosen. TOPSi@SpAcDEX particles were further functionalized with a model antigen, Trp2, to provide the final system, TOPSi@SpAcDEX-Trp2, for targeting. The nanovaccines presented high monodispersity and cytocompatibility,induced expression of co-stimulatory signals in both immortal cell lines and inperipheral blood monocytes, and enhanced the secretion of IFN-γ. Overall, thedeveloped nanovaccines showed promising adjuvant properties and the possibility of encapsulating the nanosystems with materials derived from the patient's tumor cells opens new prospectives for personalized cancer therapy.

AB - In recent years, biomaterials have gained importance as vehicles and adjuvants in the formulation of novel cancer vaccines. Among the different biomaterials proposed porous silicon (PSi) provides a versatile biocompatible platform for various biomedical applications. The aim of this work was to develop a multistage nanovaccine constructed from biomaterials and a biological cancer cell membrane (CCM), and to assess its immunostimulative properties. Glass-capillary microfluidic nanoprecipitation technique was used to produce the initial two layers of a nanovaccine. TOPSi nanoparticles were encapsulated into acetalated dextran (AcDEX) or sperminemodified AcDEX (SpAcDEX) and further co-extruded together with vesicles derived from CCM to obtain the final core-shell system (TOPSi@AcDEX@CCM), in order to combine the antigenic composition of tumor lysate with the adjuvant properties of the PSi nanoparticles chosen. TOPSi@SpAcDEX particles were further functionalized with a model antigen, Trp2, to provide the final system, TOPSi@SpAcDEX-Trp2, for targeting. The nanovaccines presented high monodispersity and cytocompatibility,induced expression of co-stimulatory signals in both immortal cell lines and inperipheral blood monocytes, and enhanced the secretion of IFN-γ. Overall, thedeveloped nanovaccines showed promising adjuvant properties and the possibility of encapsulating the nanosystems with materials derived from the patient's tumor cells opens new prospectives for personalized cancer therapy.

KW - 116 Chemical sciences

KW - 221 Nano-technology

KW - 317 Pharmacy

KW - DELIVERY PLATFORM

KW - DRUG-DELIVERY

KW - SYNTHETIC NANOPARTICLES

KW - ANTITUMOR IMMUNITY

KW - CROSS-PRESENTATION

KW - SURFACE-CHEMISTRY

KW - DENDRITIC CELLS

KW - MICROPARTICLES

KW - VACCINES

KW - SIZE

UR - http://onlinelibrary.wiley.com/journal/10.1002/%28ISSN%291521-4095

U2 - 10.1002/adma.201603239

DO - 10.1002/adma.201603239

M3 - Article

VL - 29

JO - Advanced Materials

JF - Advanced Materials

SN - 0935-9648

IS - 7

M1 - 1603239

ER -