Novel mutants of Erwinia carotovora subsp. carotovora defective in the production of plant cell wall degrading enzymes generated by Mu transpososome-mediated insertion mutagenesis

Eve Laasik, Merli Ojarand, Maria Pajunen, Harri Savilahti, Andres Mäe

    Research output: Contribution to journalArticleScientificpeer-review

    Abstract

    As in Enwinia carotovora subsp. carotovora the regulation details of the main virulence factors, encoding extracellular enzymes that degrade the plant cell wall, is only rudimentally understood, we performed a genetic screen to identify novel candidate genes involved in the process. Initially, we used Mu transpososome-mediated mutagenesis approach to generate a comprehensive transposon insertion mutant library of ca. 10000 clones and screened the clones for the loss of extracellular enzyme production. Extracellular enzymes production was abolished by mutations in the chromosomal hel(Ecc), trkA(Ecc) yheL(Ecc), gls(Ecc), igaA(Ecc) and cysQ(Ecc) genes. The findings reported here demonstrate that we have isolated six new representatives that belong to the pool of genes modulating the production of virulence factors in E. carotovora. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
    Original languageEnglish
    JournalFEMS Microbiology Letters
    Volume243
    Pages (from-to)93-99
    Number of pages7
    ISSN0378-1097
    DOIs
    Publication statusPublished - 2005
    MoE publication typeA1 Journal article-refereed

    Cite this

    @article{fde5bfa6592d4558ac39bbd370dd2569,
    title = "Novel mutants of Erwinia carotovora subsp. carotovora defective in the production of plant cell wall degrading enzymes generated by Mu transpososome-mediated insertion mutagenesis",
    abstract = "As in Enwinia carotovora subsp. carotovora the regulation details of the main virulence factors, encoding extracellular enzymes that degrade the plant cell wall, is only rudimentally understood, we performed a genetic screen to identify novel candidate genes involved in the process. Initially, we used Mu transpososome-mediated mutagenesis approach to generate a comprehensive transposon insertion mutant library of ca. 10000 clones and screened the clones for the loss of extracellular enzyme production. Extracellular enzymes production was abolished by mutations in the chromosomal hel(Ecc), trkA(Ecc) yheL(Ecc), gls(Ecc), igaA(Ecc) and cysQ(Ecc) genes. The findings reported here demonstrate that we have isolated six new representatives that belong to the pool of genes modulating the production of virulence factors in E. carotovora. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.",
    author = "Eve Laasik and Merli Ojarand and Maria Pajunen and Harri Savilahti and Andres M{\"a}e",
    year = "2005",
    doi = "10.1016/j.femsle.2004.11.045",
    language = "English",
    volume = "243",
    pages = "93--99",
    journal = "FEMS Microbiology Letters",
    issn = "0378-1097",
    publisher = "Oxford University Press",

    }

    Novel mutants of Erwinia carotovora subsp. carotovora defective in the production of plant cell wall degrading enzymes generated by Mu transpososome-mediated insertion mutagenesis. / Laasik, Eve; Ojarand, Merli; Pajunen, Maria; Savilahti, Harri; Mäe, Andres.

    In: FEMS Microbiology Letters, Vol. 243, 2005, p. 93-99.

    Research output: Contribution to journalArticleScientificpeer-review

    TY - JOUR

    T1 - Novel mutants of Erwinia carotovora subsp. carotovora defective in the production of plant cell wall degrading enzymes generated by Mu transpososome-mediated insertion mutagenesis

    AU - Laasik, Eve

    AU - Ojarand, Merli

    AU - Pajunen, Maria

    AU - Savilahti, Harri

    AU - Mäe, Andres

    PY - 2005

    Y1 - 2005

    N2 - As in Enwinia carotovora subsp. carotovora the regulation details of the main virulence factors, encoding extracellular enzymes that degrade the plant cell wall, is only rudimentally understood, we performed a genetic screen to identify novel candidate genes involved in the process. Initially, we used Mu transpososome-mediated mutagenesis approach to generate a comprehensive transposon insertion mutant library of ca. 10000 clones and screened the clones for the loss of extracellular enzyme production. Extracellular enzymes production was abolished by mutations in the chromosomal hel(Ecc), trkA(Ecc) yheL(Ecc), gls(Ecc), igaA(Ecc) and cysQ(Ecc) genes. The findings reported here demonstrate that we have isolated six new representatives that belong to the pool of genes modulating the production of virulence factors in E. carotovora. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

    AB - As in Enwinia carotovora subsp. carotovora the regulation details of the main virulence factors, encoding extracellular enzymes that degrade the plant cell wall, is only rudimentally understood, we performed a genetic screen to identify novel candidate genes involved in the process. Initially, we used Mu transpososome-mediated mutagenesis approach to generate a comprehensive transposon insertion mutant library of ca. 10000 clones and screened the clones for the loss of extracellular enzyme production. Extracellular enzymes production was abolished by mutations in the chromosomal hel(Ecc), trkA(Ecc) yheL(Ecc), gls(Ecc), igaA(Ecc) and cysQ(Ecc) genes. The findings reported here demonstrate that we have isolated six new representatives that belong to the pool of genes modulating the production of virulence factors in E. carotovora. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

    U2 - 10.1016/j.femsle.2004.11.045

    DO - 10.1016/j.femsle.2004.11.045

    M3 - Article

    VL - 243

    SP - 93

    EP - 99

    JO - FEMS Microbiology Letters

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    SN - 0378-1097

    ER -