On the reactions of two fungal laccases differing in their redox potential with lignin model compounds: products and their rate of formation

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    Abstract

    Laccases (EC 1.10.3.2) are multicopper oxidases able to oxidize phenolic compounds such as lignin-related polyphenols. Since the discovery that so-called mediators effectively extend the family of laccase substrates, direct interactions between lignin-like materials and laccase have gained much less attention. In this work, the aim was to characterize oxidation products formed in direct laccase-catalyzed oxidation of different guaiacylic and syringylic lignin model compounds with two different laccases: a low redox potential Melanocarpus albomyces laccase and a high redox potential Trametes hirsuta laccase. By following the formation of different, mainly biphenylic (5-5)
    and benzylic oxidation products, it was found that although both of these enzymes generated practically the same pattern of products with particular types of syringyl and guaiacyl compounds, in some cases a clear difference in the rates of their formation was observed. The results also confirm
    further to the suggestions that syringylic compounds are able to act as mediators in their own oxidation reactions and also that in some instances acetylation of phenolic material may produce altered, unexpected structures.
    Original languageEnglish
    JournalJournal of Agricultural and Food Chemistry
    Volume57
    Issue number18
    Pages (from-to)8357-8365
    Number of pages9
    ISSN0021-8561
    DOIs
    Publication statusPublished - 2009
    MoE publication typeA1 Journal article-refereed

    Fields of Science

    • Laccase
    • lignin
    • model compound
    • guaiacyl
    • syringyl
    • oxidation
    • product structure
    • mass spectrometry
    • electrospray
    • Trametes hirsuta
    • Melanocarpus albomyces
    • BETA-GUAIACYL ETHER
    • CORIOLUS-VERSICOLOR
    • MEDIATOR SYSTEM
    • DEGRADATION
    • OXIDATION
    • DEHYDROGENATION
    • ENZYMES
    • 116 Chemical sciences

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