Orexin receptors couple to Ca2+ channels different from store-operated Ca2+ channels

J. P. Kukkonen; K. E. Akerman

Orexin receptors couple to Ca2+ channels different from store-operated Ca2+ channels

Research output: Contribution to journal › Article › Scientific › peer-review

Abstract

We have investigated Ca2+ release and receptor- and store-operated Ca2+ influxes in Chinese hamster ovary-K1 cells expressing human OX1 orexin receptor. Receptor-operated Ca2+ influx-response to 3 nM orexin-A was not affected by Gd3+ or 2-APB (2-aminoethoxydiphenyl borate), but was inhibited by Ni2+. Store-operated Ca2+ influx was blocked by Ni2+, Gd3+ and 2-APB, whereas the thapsigargin-induced release was unaffected. 2-APB did not block inositol-1,4,5- trisphosphate-dependent Ca2+ release in these cells. Thus, low concentrations of orexin-A cause activation of two Ca2+ influxes in the cells: primarily, a receptor-operated Ca2+ influx, and secondarily, a store-depletion activated Ca2+ influx, which is subsequent to receptor-activated Ca2+ influx and the therewith-caused IP3 production. The results show that these two rely on different molecular entities.

Original language	English
Journal	NeuroReport
Volume	12
Issue number	9
Pages (from-to)	2017-20
Number of pages	4
ISSN	0959-4965
Publication status	Published - 2001
MoE publication type	A1 Journal article-refereed

Bibliographical note

Kukkonen, J P Akerman, K E Research Support, Non-U.S. Gov't England Neuroreport Neuroreport. 2001 Jul 3;12(9):2017-20.

Cite this

@article{67ee7e5fa50b43cd9463f06c1730c018,

title = "Orexin receptors couple to Ca2+ channels different from store-operated Ca2+ channels",

abstract = "We have investigated Ca2+ release and receptor- and store-operated Ca2+ influxes in Chinese hamster ovary-K1 cells expressing human OX1 orexin receptor. Receptor-operated Ca2+ influx-response to 3 nM orexin-A was not affected by Gd3+ or 2-APB (2-aminoethoxydiphenyl borate), but was inhibited by Ni2+. Store-operated Ca2+ influx was blocked by Ni2+, Gd3+ and 2-APB, whereas the thapsigargin-induced release was unaffected. 2-APB did not block inositol-1,4,5- trisphosphate-dependent Ca2+ release in these cells. Thus, low concentrations of orexin-A cause activation of two Ca2+ influxes in the cells: primarily, a receptor-operated Ca2+ influx, and secondarily, a store-depletion activated Ca2+ influx, which is subsequent to receptor-activated Ca2+ influx and the therewith-caused IP3 production. The results show that these two rely on different molecular entities.",

keywords = "Animals CHO Cells/drug effects/*metabolism Calcium Channel Blockers/pharmacology Calcium Channels/drug effects/*metabolism Calcium Signaling/drug effects/*physiology Carrier Proteins/metabolism/pharmacology Cricetinae Enzyme Inhibitors/pharmacology *Intracellular Signaling Peptides and Proteins Neuropeptides/metabolism/pharmacology Receptors, G-Protein-Coupled Receptors, Neuropeptide/drug effects/*metabolism Thapsigargin/pharmacology",

author = "Kukkonen, \{J. P.\} and Akerman, \{K. E.\}",

note = "Kukkonen, J P Akerman, K E Research Support, Non-U.S. Gov't England Neuroreport Neuroreport. 2001 Jul 3;12(9):2017-20. ",

year = "2001",

language = "English",

volume = "12",

pages = "2017--20",

journal = "NeuroReport",

issn = "0959-4965",

publisher = "Lippincott Williams and Wilkins",

number = "9",

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TY - JOUR

T1 - Orexin receptors couple to Ca2+ channels different from store-operated Ca2+ channels

AU - Kukkonen, J. P.

AU - Akerman, K. E.

N1 - Kukkonen, J P Akerman, K E Research Support, Non-U.S. Gov't England Neuroreport Neuroreport. 2001 Jul 3;12(9):2017-20.

PY - 2001

Y1 - 2001

N2 - We have investigated Ca2+ release and receptor- and store-operated Ca2+ influxes in Chinese hamster ovary-K1 cells expressing human OX1 orexin receptor. Receptor-operated Ca2+ influx-response to 3 nM orexin-A was not affected by Gd3+ or 2-APB (2-aminoethoxydiphenyl borate), but was inhibited by Ni2+. Store-operated Ca2+ influx was blocked by Ni2+, Gd3+ and 2-APB, whereas the thapsigargin-induced release was unaffected. 2-APB did not block inositol-1,4,5- trisphosphate-dependent Ca2+ release in these cells. Thus, low concentrations of orexin-A cause activation of two Ca2+ influxes in the cells: primarily, a receptor-operated Ca2+ influx, and secondarily, a store-depletion activated Ca2+ influx, which is subsequent to receptor-activated Ca2+ influx and the therewith-caused IP3 production. The results show that these two rely on different molecular entities.

AB - We have investigated Ca2+ release and receptor- and store-operated Ca2+ influxes in Chinese hamster ovary-K1 cells expressing human OX1 orexin receptor. Receptor-operated Ca2+ influx-response to 3 nM orexin-A was not affected by Gd3+ or 2-APB (2-aminoethoxydiphenyl borate), but was inhibited by Ni2+. Store-operated Ca2+ influx was blocked by Ni2+, Gd3+ and 2-APB, whereas the thapsigargin-induced release was unaffected. 2-APB did not block inositol-1,4,5- trisphosphate-dependent Ca2+ release in these cells. Thus, low concentrations of orexin-A cause activation of two Ca2+ influxes in the cells: primarily, a receptor-operated Ca2+ influx, and secondarily, a store-depletion activated Ca2+ influx, which is subsequent to receptor-activated Ca2+ influx and the therewith-caused IP3 production. The results show that these two rely on different molecular entities.

KW - Animals CHO Cells/drug effects/metabolism Calcium Channel Blockers/pharmacology Calcium Channels/drug effects/metabolism Calcium Signaling/drug effects/physiology Carrier Proteins/metabolism/pharmacology Cricetinae Enzyme Inhibitors/pharmacology Intracell

M3 - Article

SN - 0959-4965

VL - 12

SP - 2017

EP - 2020

JO - NeuroReport

JF - NeuroReport

IS - 9

ER -