Pretargeted PET Imaging of trans-Cyclooctene-Modified Porous Silicon Nanoparticles

Outi Keinänen, Ermei M. Mäkilä, Rici Lindgren, Helena Virtanen, Heidi Liljenbäck, Vesa Oikonen, Mirkka Sarparanta, Carla Molthoff, Albert D. Windhorst, Anne Roivainen, Jarno J. Salonen, Anu J. Airaksinen

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Pretargeted positron emission tomography (PET) imaging based on bioorthogonal chemical reactions has proven its potential in immunoimaging. It may also have great potential in nanotheranostic applications. Here, we report the first successful pretargeted PET imaging of trans-cyclooctene-modified mesoporous silicon nanoparticles, using 18F-labeled tetrazine as a tracer. The inverse electron-demand Diels–Alder cycloaddition (IEDDA) reaction was fast, resulting in high radioactivity accumulation in the expected organs within 10 min after the administration of the tracer. The highest target-to-background ratio was achieved 120 min after the tracer injection. A clear correlation between the efficiency of the in vivo IEDDA labeling reaction and the injected amount of the tracer was observed. The radioactivity accumulation decreased with the increased amount of the co-injected carrier, indicating saturation in the reaction sites. This finding was supported by the in vitro results. Our study suggests that pretargeted imaging has excellent potential in nanotheranostic PET imaging when using high-specific-activity tracers.
Original languageEnglish
JournalACS Omega
Volume2
Issue number1
Pages (from-to)62-69
Number of pages8
ISSN2470-1343
DOIs
Publication statusPublished - 31 Jan 2017
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 116 Chemical sciences
  • RADIOPHARMACEUTICALS

Cite this

Keinänen, O., Mäkilä, E. M., Lindgren, R., Virtanen, H., Liljenbäck, H., Oikonen, V., ... Airaksinen, A. J. (2017). Pretargeted PET Imaging of trans-Cyclooctene-Modified Porous Silicon Nanoparticles. ACS Omega, 2(1), 62-69. https://doi.org/10.1021/acsomega.6b00269
Keinänen, Outi ; Mäkilä, Ermei M. ; Lindgren, Rici ; Virtanen, Helena ; Liljenbäck, Heidi ; Oikonen, Vesa ; Sarparanta, Mirkka ; Molthoff, Carla ; Windhorst, Albert D. ; Roivainen, Anne ; Salonen, Jarno J. ; Airaksinen, Anu J. / Pretargeted PET Imaging of trans-Cyclooctene-Modified Porous Silicon Nanoparticles. In: ACS Omega. 2017 ; Vol. 2, No. 1. pp. 62-69.
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title = "Pretargeted PET Imaging of trans-Cyclooctene-Modified Porous Silicon Nanoparticles",
abstract = "Pretargeted positron emission tomography (PET) imaging based on bioorthogonal chemical reactions has proven its potential in immunoimaging. It may also have great potential in nanotheranostic applications. Here, we report the first successful pretargeted PET imaging of trans-cyclooctene-modified mesoporous silicon nanoparticles, using 18F-labeled tetrazine as a tracer. The inverse electron-demand Diels–Alder cycloaddition (IEDDA) reaction was fast, resulting in high radioactivity accumulation in the expected organs within 10 min after the administration of the tracer. The highest target-to-background ratio was achieved 120 min after the tracer injection. A clear correlation between the efficiency of the in vivo IEDDA labeling reaction and the injected amount of the tracer was observed. The radioactivity accumulation decreased with the increased amount of the co-injected carrier, indicating saturation in the reaction sites. This finding was supported by the in vitro results. Our study suggests that pretargeted imaging has excellent potential in nanotheranostic PET imaging when using high-specific-activity tracers.",
keywords = "116 Chemical sciences, RADIOPHARMACEUTICALS",
author = "Outi Kein{\"a}nen and M{\"a}kil{\"a}, {Ermei M.} and Rici Lindgren and Helena Virtanen and Heidi Liljenb{\"a}ck and Vesa Oikonen and Mirkka Sarparanta and Carla Molthoff and Windhorst, {Albert D.} and Anne Roivainen and Salonen, {Jarno J.} and Airaksinen, {Anu J.}",
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Keinänen, O, Mäkilä, EM, Lindgren, R, Virtanen, H, Liljenbäck, H, Oikonen, V, Sarparanta, M, Molthoff, C, Windhorst, AD, Roivainen, A, Salonen, JJ & Airaksinen, AJ 2017, 'Pretargeted PET Imaging of trans-Cyclooctene-Modified Porous Silicon Nanoparticles', ACS Omega, vol. 2, no. 1, pp. 62-69. https://doi.org/10.1021/acsomega.6b00269

Pretargeted PET Imaging of trans-Cyclooctene-Modified Porous Silicon Nanoparticles. / Keinänen, Outi; Mäkilä, Ermei M.; Lindgren, Rici; Virtanen, Helena; Liljenbäck, Heidi; Oikonen, Vesa; Sarparanta, Mirkka; Molthoff, Carla; Windhorst, Albert D.; Roivainen, Anne; Salonen, Jarno J.; Airaksinen, Anu J.

In: ACS Omega, Vol. 2, No. 1, 31.01.2017, p. 62-69.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Pretargeted PET Imaging of trans-Cyclooctene-Modified Porous Silicon Nanoparticles

AU - Keinänen, Outi

AU - Mäkilä, Ermei M.

AU - Lindgren, Rici

AU - Virtanen, Helena

AU - Liljenbäck, Heidi

AU - Oikonen, Vesa

AU - Sarparanta, Mirkka

AU - Molthoff, Carla

AU - Windhorst, Albert D.

AU - Roivainen, Anne

AU - Salonen, Jarno J.

AU - Airaksinen, Anu J.

N1 - doi: 10.1021/acsomega.6b00269 Volume: Proceeding volume:

PY - 2017/1/31

Y1 - 2017/1/31

N2 - Pretargeted positron emission tomography (PET) imaging based on bioorthogonal chemical reactions has proven its potential in immunoimaging. It may also have great potential in nanotheranostic applications. Here, we report the first successful pretargeted PET imaging of trans-cyclooctene-modified mesoporous silicon nanoparticles, using 18F-labeled tetrazine as a tracer. The inverse electron-demand Diels–Alder cycloaddition (IEDDA) reaction was fast, resulting in high radioactivity accumulation in the expected organs within 10 min after the administration of the tracer. The highest target-to-background ratio was achieved 120 min after the tracer injection. A clear correlation between the efficiency of the in vivo IEDDA labeling reaction and the injected amount of the tracer was observed. The radioactivity accumulation decreased with the increased amount of the co-injected carrier, indicating saturation in the reaction sites. This finding was supported by the in vitro results. Our study suggests that pretargeted imaging has excellent potential in nanotheranostic PET imaging when using high-specific-activity tracers.

AB - Pretargeted positron emission tomography (PET) imaging based on bioorthogonal chemical reactions has proven its potential in immunoimaging. It may also have great potential in nanotheranostic applications. Here, we report the first successful pretargeted PET imaging of trans-cyclooctene-modified mesoporous silicon nanoparticles, using 18F-labeled tetrazine as a tracer. The inverse electron-demand Diels–Alder cycloaddition (IEDDA) reaction was fast, resulting in high radioactivity accumulation in the expected organs within 10 min after the administration of the tracer. The highest target-to-background ratio was achieved 120 min after the tracer injection. A clear correlation between the efficiency of the in vivo IEDDA labeling reaction and the injected amount of the tracer was observed. The radioactivity accumulation decreased with the increased amount of the co-injected carrier, indicating saturation in the reaction sites. This finding was supported by the in vitro results. Our study suggests that pretargeted imaging has excellent potential in nanotheranostic PET imaging when using high-specific-activity tracers.

KW - 116 Chemical sciences

KW - RADIOPHARMACEUTICALS

U2 - 10.1021/acsomega.6b00269

DO - 10.1021/acsomega.6b00269

M3 - Article

VL - 2

SP - 62

EP - 69

JO - ACS Omega

JF - ACS Omega

SN - 2470-1343

IS - 1

ER -

Keinänen O, Mäkilä EM, Lindgren R, Virtanen H, Liljenbäck H, Oikonen V et al. Pretargeted PET Imaging of trans-Cyclooctene-Modified Porous Silicon Nanoparticles. ACS Omega. 2017 Jan 31;2(1):62-69. https://doi.org/10.1021/acsomega.6b00269