Quantitative changes in perineuronal nets in development and posttraumatic condition

Nikita Lipachev, Nikita Arnst, Anastasiia Melnikova, Harri Jäälinoja, Anastasiya Kochneva, Alexander Zhigalov, Natalia Kulesskaya, Albert V. Aganov, Mikhail Mavlikeev, Heikki Rauvala, Andrey P. Kiyasov, Mikhail Paveliev

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Perineuronal net (PNN) is a highly structured portion of the CNS extracellular matrix (ECM) regulating synaptic plasticity and a range of pathologic conditions including posttraumatic regeneration and epilepsy. Here we studied Wisteria floribunda agglutinin-stained histological sections to quantify the PNN size and enrichment of chondroitin sulfates in mouse brain and spinal cord. Somatosensory cortex sections were examined during the period of PNN establishment at postnatal days 14, 21 and 28. The single cell PNN size and the chondroitin sulfate intensity were quantified for all cortex layers and specifically for the cortical layer IV which has the highest density of PNN-positive neurons. We demonstrate that the chondroitin sulfate proteoglycan staining intensity is increased between P14 and P28 while the PNN size remains unchanged. We then addressed posttraumatic changes of the PNN expression in laminae 6 and 7 of cervical spinal cord following hemisection injury. We demonstrate increase of the chondroitin sulfate content at 1.6–1.8 mm rostrally from the injury site and increase of the density of PNN-bearing cells at 0.4–1.2 mm caudally from the injury site. We further demonstrate decrease of the single cell PNN area at 0.2 mm caudally from the injury site suggesting that the PNN ECM takes part in the posttraumatic tissue rearrangement in the spinal cord. Our results demonstrate new insights on the PNN structure dynamics in the developing and posttraumatic CNS.
Original languageEnglish
JournalJournal of Molecular Histology
Volume50
Issue number3
Pages (from-to)203-216
Number of pages14
ISSN1567-2387
DOIs
Publication statusPublished - Jun 2019
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 3112 Neurosciences
  • Perineuronal nets
  • Extracellular matrix
  • Somatosensory cortex
  • Brain development
  • Spinal cord injury
  • CHONDROITIN SULFATE PROTEOGLYCANS
  • SPINAL-CORD-INJURY
  • EXTRACELLULAR-MATRIX
  • FUNCTIONAL REGENERATION
  • TENASCIN-R
  • PLASTICITY
  • EXPRESSION
  • GROWTH
  • MOUSE
  • HYALURONAN

Cite this

Lipachev, Nikita ; Arnst, Nikita ; Melnikova, Anastasiia ; Jäälinoja, Harri ; Kochneva, Anastasiya ; Zhigalov, Alexander ; Kulesskaya, Natalia ; Aganov, Albert V. ; Mavlikeev, Mikhail ; Rauvala, Heikki ; Kiyasov, Andrey P. ; Paveliev, Mikhail. / Quantitative changes in perineuronal nets in development and posttraumatic condition. In: Journal of Molecular Histology. 2019 ; Vol. 50, No. 3. pp. 203-216.
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abstract = "Perineuronal net (PNN) is a highly structured portion of the CNS extracellular matrix (ECM) regulating synaptic plasticity and a range of pathologic conditions including posttraumatic regeneration and epilepsy. Here we studied Wisteria floribunda agglutinin-stained histological sections to quantify the PNN size and enrichment of chondroitin sulfates in mouse brain and spinal cord. Somatosensory cortex sections were examined during the period of PNN establishment at postnatal days 14, 21 and 28. The single cell PNN size and the chondroitin sulfate intensity were quantified for all cortex layers and specifically for the cortical layer IV which has the highest density of PNN-positive neurons. We demonstrate that the chondroitin sulfate proteoglycan staining intensity is increased between P14 and P28 while the PNN size remains unchanged. We then addressed posttraumatic changes of the PNN expression in laminae 6 and 7 of cervical spinal cord following hemisection injury. We demonstrate increase of the chondroitin sulfate content at 1.6–1.8 mm rostrally from the injury site and increase of the density of PNN-bearing cells at 0.4–1.2 mm caudally from the injury site. We further demonstrate decrease of the single cell PNN area at 0.2 mm caudally from the injury site suggesting that the PNN ECM takes part in the posttraumatic tissue rearrangement in the spinal cord. Our results demonstrate new insights on the PNN structure dynamics in the developing and posttraumatic CNS.",
keywords = "3112 Neurosciences, Perineuronal nets, Extracellular matrix, Somatosensory cortex, Brain development, Spinal cord injury, CHONDROITIN SULFATE PROTEOGLYCANS, SPINAL-CORD-INJURY, EXTRACELLULAR-MATRIX, FUNCTIONAL REGENERATION, TENASCIN-R, PLASTICITY, EXPRESSION, GROWTH, MOUSE, HYALURONAN",
author = "Nikita Lipachev and Nikita Arnst and Anastasiia Melnikova and Harri J{\"a}{\"a}linoja and Anastasiya Kochneva and Alexander Zhigalov and Natalia Kulesskaya and Aganov, {Albert V.} and Mikhail Mavlikeev and Heikki Rauvala and Kiyasov, {Andrey P.} and Mikhail Paveliev",
year = "2019",
month = "6",
doi = "10.1007/s10735-019-09818-y",
language = "English",
volume = "50",
pages = "203--216",
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Lipachev, N, Arnst, N, Melnikova, A, Jäälinoja, H, Kochneva, A, Zhigalov, A, Kulesskaya, N, Aganov, AV, Mavlikeev, M, Rauvala, H, Kiyasov, AP & Paveliev, M 2019, 'Quantitative changes in perineuronal nets in development and posttraumatic condition' Journal of Molecular Histology, vol. 50, no. 3, pp. 203-216. https://doi.org/10.1007/s10735-019-09818-y

Quantitative changes in perineuronal nets in development and posttraumatic condition. / Lipachev, Nikita; Arnst, Nikita; Melnikova, Anastasiia; Jäälinoja, Harri; Kochneva, Anastasiya; Zhigalov, Alexander; Kulesskaya, Natalia; Aganov, Albert V.; Mavlikeev, Mikhail; Rauvala, Heikki; Kiyasov, Andrey P.; Paveliev, Mikhail.

In: Journal of Molecular Histology, Vol. 50, No. 3, 06.2019, p. 203-216.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Quantitative changes in perineuronal nets in development and posttraumatic condition

AU - Lipachev, Nikita

AU - Arnst, Nikita

AU - Melnikova, Anastasiia

AU - Jäälinoja, Harri

AU - Kochneva, Anastasiya

AU - Zhigalov, Alexander

AU - Kulesskaya, Natalia

AU - Aganov, Albert V.

AU - Mavlikeev, Mikhail

AU - Rauvala, Heikki

AU - Kiyasov, Andrey P.

AU - Paveliev, Mikhail

PY - 2019/6

Y1 - 2019/6

N2 - Perineuronal net (PNN) is a highly structured portion of the CNS extracellular matrix (ECM) regulating synaptic plasticity and a range of pathologic conditions including posttraumatic regeneration and epilepsy. Here we studied Wisteria floribunda agglutinin-stained histological sections to quantify the PNN size and enrichment of chondroitin sulfates in mouse brain and spinal cord. Somatosensory cortex sections were examined during the period of PNN establishment at postnatal days 14, 21 and 28. The single cell PNN size and the chondroitin sulfate intensity were quantified for all cortex layers and specifically for the cortical layer IV which has the highest density of PNN-positive neurons. We demonstrate that the chondroitin sulfate proteoglycan staining intensity is increased between P14 and P28 while the PNN size remains unchanged. We then addressed posttraumatic changes of the PNN expression in laminae 6 and 7 of cervical spinal cord following hemisection injury. We demonstrate increase of the chondroitin sulfate content at 1.6–1.8 mm rostrally from the injury site and increase of the density of PNN-bearing cells at 0.4–1.2 mm caudally from the injury site. We further demonstrate decrease of the single cell PNN area at 0.2 mm caudally from the injury site suggesting that the PNN ECM takes part in the posttraumatic tissue rearrangement in the spinal cord. Our results demonstrate new insights on the PNN structure dynamics in the developing and posttraumatic CNS.

AB - Perineuronal net (PNN) is a highly structured portion of the CNS extracellular matrix (ECM) regulating synaptic plasticity and a range of pathologic conditions including posttraumatic regeneration and epilepsy. Here we studied Wisteria floribunda agglutinin-stained histological sections to quantify the PNN size and enrichment of chondroitin sulfates in mouse brain and spinal cord. Somatosensory cortex sections were examined during the period of PNN establishment at postnatal days 14, 21 and 28. The single cell PNN size and the chondroitin sulfate intensity were quantified for all cortex layers and specifically for the cortical layer IV which has the highest density of PNN-positive neurons. We demonstrate that the chondroitin sulfate proteoglycan staining intensity is increased between P14 and P28 while the PNN size remains unchanged. We then addressed posttraumatic changes of the PNN expression in laminae 6 and 7 of cervical spinal cord following hemisection injury. We demonstrate increase of the chondroitin sulfate content at 1.6–1.8 mm rostrally from the injury site and increase of the density of PNN-bearing cells at 0.4–1.2 mm caudally from the injury site. We further demonstrate decrease of the single cell PNN area at 0.2 mm caudally from the injury site suggesting that the PNN ECM takes part in the posttraumatic tissue rearrangement in the spinal cord. Our results demonstrate new insights on the PNN structure dynamics in the developing and posttraumatic CNS.

KW - 3112 Neurosciences

KW - Perineuronal nets

KW - Extracellular matrix

KW - Somatosensory cortex

KW - Brain development

KW - Spinal cord injury

KW - CHONDROITIN SULFATE PROTEOGLYCANS

KW - SPINAL-CORD-INJURY

KW - EXTRACELLULAR-MATRIX

KW - FUNCTIONAL REGENERATION

KW - TENASCIN-R

KW - PLASTICITY

KW - EXPRESSION

KW - GROWTH

KW - MOUSE

KW - HYALURONAN

U2 - 10.1007/s10735-019-09818-y

DO - 10.1007/s10735-019-09818-y

M3 - Article

VL - 50

SP - 203

EP - 216

JO - Journal of Molecular Histology

JF - Journal of Molecular Histology

SN - 1567-2379

IS - 3

ER -