Quantitative, equal carbon response HSQC experiment, QEC-HSQC

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Abstract

Quantitative NMR has become increasingly useful and popular in recent years, with many new and emerging applications in metabolomics, quality control, reaction monitoring and other types of mixture analysis. While sensitive and simple to acquire, the low resolving power of 1D H-1 NMR spectra can be a limiting factor when analyzing complex mixtures. This drawback can be solved by observing a different type of nuclei offering improved resolution or with multidimensional experiments, such as HSQC. In this paper, we present a novel Quantitative, Equal Carbon HSQC (QEC-HSQC) experiment providing an equal response across different type of carbons regardless of the number of attached protons, in addition to an uniform response over a wide range of (1)J(CH) couplings. This enables rapid quantification and integration over multiple signals without the need for complete resonance assignments and simplifies the integration of overlapping signals. (C) 2016 Elsevier Inc All rights reserved.
Original languageEnglish
JournalJournal of Magnetic Resonance
Volume271
Pages (from-to)34-39
Number of pages6
ISSN1090-7807
DOIs
Publication statusPublished - Oct 2016
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 116 Chemical sciences

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