RCD1-DREB2A interaction in leaf senescence and stress responses in Arabidopsis thaliana

Julia P. Vainonen, Pinja Jaspers, Michael Wrzaczek, Airi Lamminmäki, Ramesha Reddy, Lauri Vaahtera, Mikael Brosche, Jaakko Kangasjärvi

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Transcriptional regulation of gene expression is one major determinant of developmental control and stress adaptation in virtually all living organisms. In recent years numerous transcription factors controlling various aspects of plant life have been identified. The activity of transcription factors needs to be regulated to prevent unspecific, prolonged or inappropriate responses. The transcription factor DEHYDRATION-RESPONSIVE ELEMENT BINDING 2A (DREB2A) has been identified as one of the main regulators of drought and heat responses, and it is regulated through protein stability. Here we present evidence that the interaction with RADICAL-INDUCED CELL DEATH1 (RCD1) contributes to the control of DREB2A under a range of conditions. The interaction is mediated by a novel protein motif in DREB2A and a splice variant of DREB2A which lacks the interaction domain accumulates during heat stress and senescence. In addition RCD1 is rapidly degraded during heat stress, thus our results suggest that removal of RCD1 protein or the loss of the interaction domain in DREB2A appears to be required for proper DREB2A function under stress conditions.
Original languageEnglish
JournalBiochemical Journal
Volume442
Pages (from-to)573-581
Number of pages9
ISSN0264-6021
DOIs
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 1183 Plant biology, microbiology, virology

Cite this

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title = "RCD1-DREB2A interaction in leaf senescence and stress responses in Arabidopsis thaliana",
abstract = "Transcriptional regulation of gene expression is one major determinant of developmental control and stress adaptation in virtually all living organisms. In recent years numerous transcription factors controlling various aspects of plant life have been identified. The activity of transcription factors needs to be regulated to prevent unspecific, prolonged or inappropriate responses. The transcription factor DEHYDRATION-RESPONSIVE ELEMENT BINDING 2A (DREB2A) has been identified as one of the main regulators of drought and heat responses, and it is regulated through protein stability. Here we present evidence that the interaction with RADICAL-INDUCED CELL DEATH1 (RCD1) contributes to the control of DREB2A under a range of conditions. The interaction is mediated by a novel protein motif in DREB2A and a splice variant of DREB2A which lacks the interaction domain accumulates during heat stress and senescence. In addition RCD1 is rapidly degraded during heat stress, thus our results suggest that removal of RCD1 protein or the loss of the interaction domain in DREB2A appears to be required for proper DREB2A function under stress conditions.",
keywords = "1183 Plant biology, microbiology, virology",
author = "Vainonen, {Julia P.} and Pinja Jaspers and Michael Wrzaczek and Airi Lamminm{\"a}ki and Ramesha Reddy and Lauri Vaahtera and Mikael Brosche and Jaakko Kangasj{\"a}rvi",
year = "2012",
doi = "10.1042/BJ20111739",
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volume = "442",
pages = "573--581",
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RCD1-DREB2A interaction in leaf senescence and stress responses in Arabidopsis thaliana. / Vainonen, Julia P.; Jaspers, Pinja; Wrzaczek, Michael; Lamminmäki, Airi; Reddy, Ramesha; Vaahtera, Lauri; Brosche, Mikael; Kangasjärvi, Jaakko.

In: Biochemical Journal, Vol. 442, 2012, p. 573-581.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - RCD1-DREB2A interaction in leaf senescence and stress responses in Arabidopsis thaliana

AU - Vainonen, Julia P.

AU - Jaspers, Pinja

AU - Wrzaczek, Michael

AU - Lamminmäki, Airi

AU - Reddy, Ramesha

AU - Vaahtera, Lauri

AU - Brosche, Mikael

AU - Kangasjärvi, Jaakko

PY - 2012

Y1 - 2012

N2 - Transcriptional regulation of gene expression is one major determinant of developmental control and stress adaptation in virtually all living organisms. In recent years numerous transcription factors controlling various aspects of plant life have been identified. The activity of transcription factors needs to be regulated to prevent unspecific, prolonged or inappropriate responses. The transcription factor DEHYDRATION-RESPONSIVE ELEMENT BINDING 2A (DREB2A) has been identified as one of the main regulators of drought and heat responses, and it is regulated through protein stability. Here we present evidence that the interaction with RADICAL-INDUCED CELL DEATH1 (RCD1) contributes to the control of DREB2A under a range of conditions. The interaction is mediated by a novel protein motif in DREB2A and a splice variant of DREB2A which lacks the interaction domain accumulates during heat stress and senescence. In addition RCD1 is rapidly degraded during heat stress, thus our results suggest that removal of RCD1 protein or the loss of the interaction domain in DREB2A appears to be required for proper DREB2A function under stress conditions.

AB - Transcriptional regulation of gene expression is one major determinant of developmental control and stress adaptation in virtually all living organisms. In recent years numerous transcription factors controlling various aspects of plant life have been identified. The activity of transcription factors needs to be regulated to prevent unspecific, prolonged or inappropriate responses. The transcription factor DEHYDRATION-RESPONSIVE ELEMENT BINDING 2A (DREB2A) has been identified as one of the main regulators of drought and heat responses, and it is regulated through protein stability. Here we present evidence that the interaction with RADICAL-INDUCED CELL DEATH1 (RCD1) contributes to the control of DREB2A under a range of conditions. The interaction is mediated by a novel protein motif in DREB2A and a splice variant of DREB2A which lacks the interaction domain accumulates during heat stress and senescence. In addition RCD1 is rapidly degraded during heat stress, thus our results suggest that removal of RCD1 protein or the loss of the interaction domain in DREB2A appears to be required for proper DREB2A function under stress conditions.

KW - 1183 Plant biology, microbiology, virology

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VL - 442

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EP - 581

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JF - Biochemical Journal

SN - 0264-6021

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