Real-time monitoring of non-specific toxicity using a Saccharomyces cerevisiae reporter system

Anna-Liisa Välimaa, Anniina Kivistö, Marko Virta, Matti Karp

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Baker's yeast, Saccharomyces cerevisiae, is the simplest and most well-known representative of eukaryotic cells and thus a convenient model organism for evaluating toxic effects in human cells and tissues. Yeast cell sensors are easy to maintain with short generation times, which makes the analytical method of assessing antifungal toxicity cheap and less-time consuming. In this work, the toxicity of test compounds was assessed in bioassays based on bioluminescence inhibition and on traditional growth inhibition on agar plates. The model organism in both tests was a modified S. cerevisiae sensor strain that produces light when provided with D-luciferin in an insect luciferase reporter gene activity assay. The bioluminescence assay showed toxic effects for yeast cell sensor of 5,6-benzoflavone, rapamycin, nystatin and cycloheximide at concentrations of nM to mu M. In addition, arsenic compounds, cadmium chloride, copper sulfate and lead acetate were shown to be potent non-specific inhibitors of the reporter organism described here. The results from a yeast agar diffusion assay correlated with the bioluminescence assay results.
Original languageEnglish
JournalSensors
Volume8
Issue number10
Pages (from-to)6433-6447
Number of pages15
ISSN1424-8220
DOIs
Publication statusPublished - 2008
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 1182 Biochemistry, cell and molecular biology
  • 1183 Plant biology, microbiology, virology

Cite this

Välimaa, Anna-Liisa ; Kivistö, Anniina ; Virta, Marko ; Karp, Matti. / Real-time monitoring of non-specific toxicity using a Saccharomyces cerevisiae reporter system. In: Sensors. 2008 ; Vol. 8, No. 10. pp. 6433-6447.
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abstract = "Baker's yeast, Saccharomyces cerevisiae, is the simplest and most well-known representative of eukaryotic cells and thus a convenient model organism for evaluating toxic effects in human cells and tissues. Yeast cell sensors are easy to maintain with short generation times, which makes the analytical method of assessing antifungal toxicity cheap and less-time consuming. In this work, the toxicity of test compounds was assessed in bioassays based on bioluminescence inhibition and on traditional growth inhibition on agar plates. The model organism in both tests was a modified S. cerevisiae sensor strain that produces light when provided with D-luciferin in an insect luciferase reporter gene activity assay. The bioluminescence assay showed toxic effects for yeast cell sensor of 5,6-benzoflavone, rapamycin, nystatin and cycloheximide at concentrations of nM to mu M. In addition, arsenic compounds, cadmium chloride, copper sulfate and lead acetate were shown to be potent non-specific inhibitors of the reporter organism described here. The results from a yeast agar diffusion assay correlated with the bioluminescence assay results.",
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Real-time monitoring of non-specific toxicity using a Saccharomyces cerevisiae reporter system. / Välimaa, Anna-Liisa; Kivistö, Anniina; Virta, Marko; Karp, Matti.

In: Sensors, Vol. 8, No. 10, 2008, p. 6433-6447.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Real-time monitoring of non-specific toxicity using a Saccharomyces cerevisiae reporter system

AU - Välimaa, Anna-Liisa

AU - Kivistö, Anniina

AU - Virta, Marko

AU - Karp, Matti

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N2 - Baker's yeast, Saccharomyces cerevisiae, is the simplest and most well-known representative of eukaryotic cells and thus a convenient model organism for evaluating toxic effects in human cells and tissues. Yeast cell sensors are easy to maintain with short generation times, which makes the analytical method of assessing antifungal toxicity cheap and less-time consuming. In this work, the toxicity of test compounds was assessed in bioassays based on bioluminescence inhibition and on traditional growth inhibition on agar plates. The model organism in both tests was a modified S. cerevisiae sensor strain that produces light when provided with D-luciferin in an insect luciferase reporter gene activity assay. The bioluminescence assay showed toxic effects for yeast cell sensor of 5,6-benzoflavone, rapamycin, nystatin and cycloheximide at concentrations of nM to mu M. In addition, arsenic compounds, cadmium chloride, copper sulfate and lead acetate were shown to be potent non-specific inhibitors of the reporter organism described here. The results from a yeast agar diffusion assay correlated with the bioluminescence assay results.

AB - Baker's yeast, Saccharomyces cerevisiae, is the simplest and most well-known representative of eukaryotic cells and thus a convenient model organism for evaluating toxic effects in human cells and tissues. Yeast cell sensors are easy to maintain with short generation times, which makes the analytical method of assessing antifungal toxicity cheap and less-time consuming. In this work, the toxicity of test compounds was assessed in bioassays based on bioluminescence inhibition and on traditional growth inhibition on agar plates. The model organism in both tests was a modified S. cerevisiae sensor strain that produces light when provided with D-luciferin in an insect luciferase reporter gene activity assay. The bioluminescence assay showed toxic effects for yeast cell sensor of 5,6-benzoflavone, rapamycin, nystatin and cycloheximide at concentrations of nM to mu M. In addition, arsenic compounds, cadmium chloride, copper sulfate and lead acetate were shown to be potent non-specific inhibitors of the reporter organism described here. The results from a yeast agar diffusion assay correlated with the bioluminescence assay results.

KW - 1182 Biochemistry, cell and molecular biology

KW - 1183 Plant biology, microbiology, virology

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