Recent advances in applying mass spectrometry and systems biology to determine brain dynamics

Enzo Scifo, Giulio Calza, Martin Fuhrmann, Rabah Soliymani, Marc Baumann, Maciej Lalowski

Research output: Contribution to journalReview ArticleScientificpeer-review

Abstract

Neurological disorders encompass various pathologies which disrupt normal brain physiology and function. Poor understanding of their underlying molecular mechanisms and their societal burden argues for the necessity of novel prevention strategies, early diagnostic techniques and alternative treatment options to reduce the scale of their expected increase. Areas covered: This review scrutinizes mass spectrometry based approaches used to investigate brain dynamics in various conditions, including neurodegenerative and neuropsychiatric disorders. Different proteomics workflows for isolation/enrichment of specific cell populations or brain regions, sample processing; mass spectrometry technologies, for differential proteome quantitation, analysis of post-translational modifications and imaging approaches in the brain are critically deliberated. Future directions, including analysis of cellular sub-compartments, targeted MS platforms (selected/parallel reaction monitoring) and use of mass cytometry are also discussed. Expert commentary: Here, we summarize and evaluate current mass spectrometry based approaches for determining brain dynamics in health and diseases states, with a focus on neurological disorders. Furthermore, we provide insight on current trends and new MS technologies with potential to improve this analysis.
Original languageEnglish
JournalExpert review of proteomics.
Volume14
Issue number6
Pages (from-to)545-559
Number of pages15
ISSN1478-9450
DOIs
Publication statusPublished - 1 Jun 2017
MoE publication typeA2 Review article in a scientific journal

Fields of Science

  • 1182 Biochemistry, cell and molecular biology
  • PROTEOMICS
  • MASS SPECTROMETRY METHOD
  • Mass spectrometry imaging
  • 318 Medical biotechnology

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