Role of two-component systems in cold tolerance of Clostridium botulinum

Yagmur Derman, Elias Dahlsten, Hannu Korkeala

Research output: Chapter in Book/Report/Conference proceedingChapterScientificpeer-review

Abstract

Clostridium botulinum can thrive under stress conditions, and the capability of C. botulinum strains to grow and produce botulinum neurotoxin under cold stress is a substantial hazard to human health. However, the molecular mechanisms and genetic markers responsible for the cold tolerance of C. botulinum are poorly understood. This chapter focuses on the cold stress response of C. botulinum and the importance of two-component systems in cold tolerance of C. botulinum ATCC 3502 and Beluga E1. The cbo0366–cbo0365 and cbo2306–cbo2307 genes of Group I C. botulinum and the clo3403–clo3404 genes of Group II Beluga E1 show an increased relative mRNA level upon cold shock. Furthermore, an insertional inactivation of genes encoding these systems results in impaired growth in comparison to the wild-type ATCC 3502 or the Beluga E1 strains.
Original languageEnglish
Title of host publication Stress and environmental regulation of gene expression and adaptation in bacteria
EditorsFrans J. de Bruijn
Number of pages9
Volume2
Place of PublicationNew Jersey
PublisherJohn Wiley & Sons Ltd.
Publication date2016
Pages845-853
ISBN (Print)9781119004882
ISBN (Electronic)9781119004813
DOIs
Publication statusPublished - 2016
MoE publication typeA3 Book chapter

Fields of Science

  • 1183 Plant biology, microbiology, virology
  • cold
  • stress
  • two-component systems
  • Clostridium botulinum
  • CBO0366–CBO0365
  • CBO2306–CBO2307
  • CLO3403–CLO3404

Cite this

Derman, Y., Dahlsten, E., & Korkeala, H. (2016). Role of two-component systems in cold tolerance of Clostridium botulinum. In F. J. de Bruijn (Ed.), Stress and environmental regulation of gene expression and adaptation in bacteria (Vol. 2, pp. 845-853). New Jersey: John Wiley & Sons Ltd. https://doi.org/10.1002/9781119004813.ch82
Derman, Yagmur ; Dahlsten, Elias ; Korkeala, Hannu. / Role of two-component systems in cold tolerance of Clostridium botulinum. Stress and environmental regulation of gene expression and adaptation in bacteria. editor / Frans J. de Bruijn. Vol. 2 New Jersey : John Wiley & Sons Ltd. , 2016. pp. 845-853
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abstract = "Clostridium botulinum can thrive under stress conditions, and the capability of C. botulinum strains to grow and produce botulinum neurotoxin under cold stress is a substantial hazard to human health. However, the molecular mechanisms and genetic markers responsible for the cold tolerance of C. botulinum are poorly understood. This chapter focuses on the cold stress response of C. botulinum and the importance of two-component systems in cold tolerance of C. botulinum ATCC 3502 and Beluga E1. The cbo0366–cbo0365 and cbo2306–cbo2307 genes of Group I C. botulinum and the clo3403–clo3404 genes of Group II Beluga E1 show an increased relative mRNA level upon cold shock. Furthermore, an insertional inactivation of genes encoding these systems results in impaired growth in comparison to the wild-type ATCC 3502 or the Beluga E1 strains.",
keywords = "1183 Plant biology, microbiology, virology, cold , stress , two-component systems , Clostridium botulinum , CBO0366–CBO0365 , CBO2306–CBO2307 , CLO3403–CLO3404",
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Derman, Y, Dahlsten, E & Korkeala, H 2016, Role of two-component systems in cold tolerance of Clostridium botulinum. in FJ de Bruijn (ed.), Stress and environmental regulation of gene expression and adaptation in bacteria. vol. 2, John Wiley & Sons Ltd. , New Jersey, pp. 845-853. https://doi.org/10.1002/9781119004813.ch82

Role of two-component systems in cold tolerance of Clostridium botulinum. / Derman, Yagmur; Dahlsten, Elias; Korkeala, Hannu.

Stress and environmental regulation of gene expression and adaptation in bacteria. ed. / Frans J. de Bruijn. Vol. 2 New Jersey : John Wiley & Sons Ltd. , 2016. p. 845-853.

Research output: Chapter in Book/Report/Conference proceedingChapterScientificpeer-review

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AU - Derman, Yagmur

AU - Dahlsten, Elias

AU - Korkeala, Hannu

PY - 2016

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N2 - Clostridium botulinum can thrive under stress conditions, and the capability of C. botulinum strains to grow and produce botulinum neurotoxin under cold stress is a substantial hazard to human health. However, the molecular mechanisms and genetic markers responsible for the cold tolerance of C. botulinum are poorly understood. This chapter focuses on the cold stress response of C. botulinum and the importance of two-component systems in cold tolerance of C. botulinum ATCC 3502 and Beluga E1. The cbo0366–cbo0365 and cbo2306–cbo2307 genes of Group I C. botulinum and the clo3403–clo3404 genes of Group II Beluga E1 show an increased relative mRNA level upon cold shock. Furthermore, an insertional inactivation of genes encoding these systems results in impaired growth in comparison to the wild-type ATCC 3502 or the Beluga E1 strains.

AB - Clostridium botulinum can thrive under stress conditions, and the capability of C. botulinum strains to grow and produce botulinum neurotoxin under cold stress is a substantial hazard to human health. However, the molecular mechanisms and genetic markers responsible for the cold tolerance of C. botulinum are poorly understood. This chapter focuses on the cold stress response of C. botulinum and the importance of two-component systems in cold tolerance of C. botulinum ATCC 3502 and Beluga E1. The cbo0366–cbo0365 and cbo2306–cbo2307 genes of Group I C. botulinum and the clo3403–clo3404 genes of Group II Beluga E1 show an increased relative mRNA level upon cold shock. Furthermore, an insertional inactivation of genes encoding these systems results in impaired growth in comparison to the wild-type ATCC 3502 or the Beluga E1 strains.

KW - 1183 Plant biology, microbiology, virology

KW - cold

KW - stress

KW - two-component systems

KW - Clostridium botulinum

KW - CBO0366–CBO0365

KW - CBO2306–CBO2307

KW - CLO3403–CLO3404

U2 - 10.1002/9781119004813.ch82

DO - 10.1002/9781119004813.ch82

M3 - Chapter

SN - 9781119004882

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BT - Stress and environmental regulation of gene expression and adaptation in bacteria

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PB - John Wiley & Sons Ltd.

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Derman Y, Dahlsten E, Korkeala H. Role of two-component systems in cold tolerance of Clostridium botulinum. In de Bruijn FJ, editor, Stress and environmental regulation of gene expression and adaptation in bacteria. Vol. 2. New Jersey: John Wiley & Sons Ltd. . 2016. p. 845-853 https://doi.org/10.1002/9781119004813.ch82