Segmental isotopic labeling of a 140 kDa dimeric multi-domain protein CheA from Escherichia coli by expressed protein ligation and protein trans-splicing

Yuichi Minato, Takumi Ueda, Asako Machiyama, Ichio Shimada, Hideo Iwai

Research output: Contribution to journalArticleScientificpeer-review

Original languageEnglish
JournalJournal of Biomolecular NMR
Volume53
Issue number3
Pages (from-to)191-207
Number of pages17
ISSN0925-2738
DOIs
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed

Fields of Science

  • Segmental isotopic labeling
  • Intein
  • Protein splicing
  • Expressed protein ligation
  • CheA
  • Protein trans-splicing
  • NATIVE CHEMICAL LIGATION
  • NUCLEAR-MAGNETIC-RESONANCE
  • BACTERIAL CHEMOTAXIS
  • RECOMBINANT PROTEINS
  • SIGNAL-TRANSDUCTION
  • SPLICING ELEMENT
  • NMR-SPECTROSCOPY
  • HISTIDINE KINASE
  • LARGER PROTEIN
  • IN-VITRO

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