Sequencing of virulence genes shows limited genetic variability in Yersinia pseudotuberculosis

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Yersinia pseudotuberculosis is a foodborne pathogen often detected and identified using polymerase chain reaction (PCR) with primers targeted to virulence genes. Sequence variability of the virulence genes in strains representing different serotypes is unknown. Sequence variability could hinder the recognition of this pathogen by PCR and affect the host–pathogen interactions. Sequencing of inv, virF, and yadA of 18 Y. pseudotuberculosis strains showed limited variability of inv and virF, whereas the sequences of yadA varied considerably.

Original languageEnglish
JournalFoodborne Pathogens and Disease
Volume10
Issue number1
Pages (from-to)21-27
Number of pages7
ISSN1535-3141
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 413 Veterinary science

Cite this

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title = "Sequencing of virulence genes shows limited genetic variability in Yersinia pseudotuberculosis",
abstract = "Yersinia pseudotuberculosis is a foodborne pathogen often detected and identified using polymerase chain reaction (PCR) with primers targeted to virulence genes. Sequence variability of the virulence genes in strains representing different serotypes is unknown. Sequence variability could hinder the recognition of this pathogen by PCR and affect the host–pathogen interactions. Sequencing of inv, virF, and yadA of 18 Y. pseudotuberculosis strains showed limited variability of inv and virF, whereas the sequences of yadA varied considerably.",
keywords = "413 Veterinary science",
author = "Eveliina Palonen and Susanna Kangas and Panu Somervuo and Miia Lindstr{\"o}m and Maria Fredriksson-Ahomaa and Mikael Skurnik and Hannu Korkeala",
year = "2013",
doi = "10.1089/fpd.2012.1247",
language = "English",
volume = "10",
pages = "21--27",
journal = "Foodborne Pathogens and Disease",
issn = "1535-3141",
publisher = "Mary Ann Liebert",
number = "1",

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Sequencing of virulence genes shows limited genetic variability in Yersinia pseudotuberculosis. / Palonen, Eveliina; Kangas, Susanna; Somervuo, Panu; Lindström, Miia; Fredriksson-Ahomaa, Maria; Skurnik, Mikael; Korkeala, Hannu.

In: Foodborne Pathogens and Disease, Vol. 10, No. 1, 2013, p. 21-27.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Sequencing of virulence genes shows limited genetic variability in Yersinia pseudotuberculosis

AU - Palonen, Eveliina

AU - Kangas, Susanna

AU - Somervuo, Panu

AU - Lindström, Miia

AU - Fredriksson-Ahomaa, Maria

AU - Skurnik, Mikael

AU - Korkeala, Hannu

PY - 2013

Y1 - 2013

N2 - Yersinia pseudotuberculosis is a foodborne pathogen often detected and identified using polymerase chain reaction (PCR) with primers targeted to virulence genes. Sequence variability of the virulence genes in strains representing different serotypes is unknown. Sequence variability could hinder the recognition of this pathogen by PCR and affect the host–pathogen interactions. Sequencing of inv, virF, and yadA of 18 Y. pseudotuberculosis strains showed limited variability of inv and virF, whereas the sequences of yadA varied considerably.

AB - Yersinia pseudotuberculosis is a foodborne pathogen often detected and identified using polymerase chain reaction (PCR) with primers targeted to virulence genes. Sequence variability of the virulence genes in strains representing different serotypes is unknown. Sequence variability could hinder the recognition of this pathogen by PCR and affect the host–pathogen interactions. Sequencing of inv, virF, and yadA of 18 Y. pseudotuberculosis strains showed limited variability of inv and virF, whereas the sequences of yadA varied considerably.

KW - 413 Veterinary science

U2 - 10.1089/fpd.2012.1247

DO - 10.1089/fpd.2012.1247

M3 - Article

VL - 10

SP - 21

EP - 27

JO - Foodborne Pathogens and Disease

JF - Foodborne Pathogens and Disease

SN - 1535-3141

IS - 1

ER -