Sequential Antifouling Surface for Efficient Modulation of the Nanoparticle-Cell Interactions in Protein-Rich Environment

Feng Zhang, Li Kong, Dongfei Liu, Wei Li, Ermei Mäkilä, Alexandra Correia, Rici Lindgren, Jarno Salonen, Jouni Tapio Hirvonen, Hongbo Zhang, Alexander Kros, Helder Almeida Santos

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Modulating the nanoparticle (NP)-cell interactions in protein-rich environment owns increasing significance to nanomedicine. One major challenge is that the synthetic identity (such as targeting) of NPs endowed for this purpose can be adversely altered by opsonization process. The formed protein corona on NPs can cause fast clearance of NPs by macrophages and reduced cancer cell uptake. Many transformable NPs with antifouling surface, which can achieve inactive-active form conversion for cell recognition, offer great promissing to efficient modulate NP-cell interactions. However, most of the transformalbe NPs lack of a sustainable protection mechanism to aviod the influence of protein corona druing the post-transformation period, leading to the re-opsonization on NPs. Here, we design a smart transformable nanosystem with a photo-triggered switterization-induced sequential antifouling surface to efficiently reduce protein adsorption on NPs and modulate the NPs-cells interaction in protein-rich enviroment. We demonstrat that the primary PEGylated antifouling surface could protect the NPs against macrophages uptake. Futhermore, the photo-induced secondary zwitterionic antifouling surface could well preserve the targeting capacity of biotin-modified NPs during the post-transformation period in protein-rich environment. In contrast, the biotin-conjugated NPs without antifouling surface almost lost the targeting specificity in protein-rich environment.
Original languageEnglish
JournalAdvanced Therapeutics
Volume1
Issue number1
Pages (from-to)1800013
Number of pages12
DOIs
Publication statusPublished - May 2018
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 317 Pharmacy

Cite this

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title = "Sequential Antifouling Surface for Efficient Modulation of the Nanoparticle-Cell Interactions in Protein-Rich Environment",
abstract = "Modulating the nanoparticle (NP)-cell interactions in protein-rich environment owns increasing significance to nanomedicine. One major challenge is that the synthetic identity (such as targeting) of NPs endowed for this purpose can be adversely altered by opsonization process. The formed protein corona on NPs can cause fast clearance of NPs by macrophages and reduced cancer cell uptake. Many transformable NPs with antifouling surface, which can achieve inactive-active form conversion for cell recognition, offer great promissing to efficient modulate NP-cell interactions. However, most of the transformalbe NPs lack of a sustainable protection mechanism to aviod the influence of protein corona druing the post-transformation period, leading to the re-opsonization on NPs. Here, we design a smart transformable nanosystem with a photo-triggered switterization-induced sequential antifouling surface to efficiently reduce protein adsorption on NPs and modulate the NPs-cells interaction in protein-rich enviroment. We demonstrat that the primary PEGylated antifouling surface could protect the NPs against macrophages uptake. Futhermore, the photo-induced secondary zwitterionic antifouling surface could well preserve the targeting capacity of biotin-modified NPs during the post-transformation period in protein-rich environment. In contrast, the biotin-conjugated NPs without antifouling surface almost lost the targeting specificity in protein-rich environment.",
keywords = "317 Pharmacy",
author = "Feng Zhang and Li Kong and Dongfei Liu and Wei Li and Ermei M{\"a}kil{\"a} and Alexandra Correia and Rici Lindgren and Jarno Salonen and Hirvonen, {Jouni Tapio} and Hongbo Zhang and Alexander Kros and {Almeida Santos}, Helder",
year = "2018",
month = "5",
doi = "10.1002/adtp.201800013",
language = "English",
volume = "1",
pages = "1800013",
journal = "Advanced Therapeutics",
issn = "2366-3987",
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Sequential Antifouling Surface for Efficient Modulation of the Nanoparticle-Cell Interactions in Protein-Rich Environment. / Zhang, Feng; Kong, Li; Liu, Dongfei; Li, Wei; Mäkilä, Ermei; Correia, Alexandra; Lindgren, Rici; Salonen, Jarno; Hirvonen, Jouni Tapio; Zhang, Hongbo; Kros, Alexander; Almeida Santos, Helder.

In: Advanced Therapeutics, Vol. 1, No. 1, 05.2018, p. 1800013.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Sequential Antifouling Surface for Efficient Modulation of the Nanoparticle-Cell Interactions in Protein-Rich Environment

AU - Zhang, Feng

AU - Kong, Li

AU - Liu, Dongfei

AU - Li, Wei

AU - Mäkilä, Ermei

AU - Correia, Alexandra

AU - Lindgren, Rici

AU - Salonen, Jarno

AU - Hirvonen, Jouni Tapio

AU - Zhang, Hongbo

AU - Kros, Alexander

AU - Almeida Santos, Helder

PY - 2018/5

Y1 - 2018/5

N2 - Modulating the nanoparticle (NP)-cell interactions in protein-rich environment owns increasing significance to nanomedicine. One major challenge is that the synthetic identity (such as targeting) of NPs endowed for this purpose can be adversely altered by opsonization process. The formed protein corona on NPs can cause fast clearance of NPs by macrophages and reduced cancer cell uptake. Many transformable NPs with antifouling surface, which can achieve inactive-active form conversion for cell recognition, offer great promissing to efficient modulate NP-cell interactions. However, most of the transformalbe NPs lack of a sustainable protection mechanism to aviod the influence of protein corona druing the post-transformation period, leading to the re-opsonization on NPs. Here, we design a smart transformable nanosystem with a photo-triggered switterization-induced sequential antifouling surface to efficiently reduce protein adsorption on NPs and modulate the NPs-cells interaction in protein-rich enviroment. We demonstrat that the primary PEGylated antifouling surface could protect the NPs against macrophages uptake. Futhermore, the photo-induced secondary zwitterionic antifouling surface could well preserve the targeting capacity of biotin-modified NPs during the post-transformation period in protein-rich environment. In contrast, the biotin-conjugated NPs without antifouling surface almost lost the targeting specificity in protein-rich environment.

AB - Modulating the nanoparticle (NP)-cell interactions in protein-rich environment owns increasing significance to nanomedicine. One major challenge is that the synthetic identity (such as targeting) of NPs endowed for this purpose can be adversely altered by opsonization process. The formed protein corona on NPs can cause fast clearance of NPs by macrophages and reduced cancer cell uptake. Many transformable NPs with antifouling surface, which can achieve inactive-active form conversion for cell recognition, offer great promissing to efficient modulate NP-cell interactions. However, most of the transformalbe NPs lack of a sustainable protection mechanism to aviod the influence of protein corona druing the post-transformation period, leading to the re-opsonization on NPs. Here, we design a smart transformable nanosystem with a photo-triggered switterization-induced sequential antifouling surface to efficiently reduce protein adsorption on NPs and modulate the NPs-cells interaction in protein-rich enviroment. We demonstrat that the primary PEGylated antifouling surface could protect the NPs against macrophages uptake. Futhermore, the photo-induced secondary zwitterionic antifouling surface could well preserve the targeting capacity of biotin-modified NPs during the post-transformation period in protein-rich environment. In contrast, the biotin-conjugated NPs without antifouling surface almost lost the targeting specificity in protein-rich environment.

KW - 317 Pharmacy

U2 - 10.1002/adtp.201800013

DO - 10.1002/adtp.201800013

M3 - Article

VL - 1

SP - 1800013

JO - Advanced Therapeutics

JF - Advanced Therapeutics

SN - 2366-3987

IS - 1

ER -