Short oligogalacturonides induce pathogen resistance-associated gene expression in Arabidopsis thaliana

Research output: Contribution to journalArticleScientificpeer-review

Abstract


Background

Oligogalacturonides (OGs) are important components of damage-associated molecular pattern (DAMP) signaling and influence growth regulation in plants. Recent studies have focused on the impact of long OGs (degree of polymerization (DP) from 10–15), demonstrating the induction of plant defense signaling resulting in enhanced defenses to necrotrophic pathogens. To clarify the role of trimers (trimeric OGs, DP3) in DAMP signaling and their impact on plant growth regulation, we performed a transcriptomic analysis through the RNA sequencing of Arabidopsis thaliana exposed to trimers.
Results

The transcriptomic data from trimer-treated Arabidopsis seedlings indicate a clear activation of genes involved in defense signaling, phytohormone signaling and a down-regulation of genes involved in processes related to growth regulation and development. This is further accompanied with improved defenses against necrotrophic pathogens triggered by the trimer treatment, indicating that short OGs have a clear impact on plant responses, similar to those described for long OGs.
Conclusions

Our results demonstrate that trimers are indeed active elicitors of plant defenses. This is clearly indicated by the up-regulation of genes associated with plant defense signaling, accompanied with improved defenses against necrotrophic pathogens. Moreover, trimers simultaneously trigger a clear down-regulation of genes and gene sets associated with growth and development, leading to stunted seedling growth in Arabidopsis.
Keywords
Plant signaling Arabidopsis thaliana Oligogalacturonides OG Trimers Transcriptomics Defense induction Growth inhibition Disease resistance Pectobacterium carotovorum Botrytis cinerea
Original languageEnglish
Article number19
JournalBMC Plant Biology
Volume17
Issue number19
Number of pages17
ISSN1471-2229
DOIs
Publication statusPublished - 19 Jan 2017
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 1183 Plant biology, microbiology, virology

Cite this

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title = "Short oligogalacturonides induce pathogen resistance-associated gene expression in Arabidopsis thaliana",
abstract = "BackgroundOligogalacturonides (OGs) are important components of damage-associated molecular pattern (DAMP) signaling and influence growth regulation in plants. Recent studies have focused on the impact of long OGs (degree of polymerization (DP) from 10–15), demonstrating the induction of plant defense signaling resulting in enhanced defenses to necrotrophic pathogens. To clarify the role of trimers (trimeric OGs, DP3) in DAMP signaling and their impact on plant growth regulation, we performed a transcriptomic analysis through the RNA sequencing of Arabidopsis thaliana exposed to trimers.ResultsThe transcriptomic data from trimer-treated Arabidopsis seedlings indicate a clear activation of genes involved in defense signaling, phytohormone signaling and a down-regulation of genes involved in processes related to growth regulation and development. This is further accompanied with improved defenses against necrotrophic pathogens triggered by the trimer treatment, indicating that short OGs have a clear impact on plant responses, similar to those described for long OGs.ConclusionsOur results demonstrate that trimers are indeed active elicitors of plant defenses. This is clearly indicated by the up-regulation of genes associated with plant defense signaling, accompanied with improved defenses against necrotrophic pathogens. Moreover, trimers simultaneously trigger a clear down-regulation of genes and gene sets associated with growth and development, leading to stunted seedling growth in Arabidopsis.KeywordsPlant signaling Arabidopsis thaliana Oligogalacturonides OG Trimers Transcriptomics Defense induction Growth inhibition Disease resistance Pectobacterium carotovorum Botrytis cinerea",
keywords = "1183 Plant biology, microbiology, virology",
author = "Davidsson, {P{\"a}r Roland} and Martin Broberg and Kariola, {Tarja Johanna} and Sipari, {Nina Hannele} and Pirhonen, {Minna Ursula} and Palva, {Erkki Tapio}",
year = "2017",
month = "1",
day = "19",
doi = "10.1186/s12870-016-0959-1",
language = "English",
volume = "17",
journal = "BMC Plant Biology",
issn = "1471-2229",
publisher = "BMC",
number = "19",

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Short oligogalacturonides induce pathogen resistance-associated gene expression in Arabidopsis thaliana. / Davidsson, Pär Roland; Broberg, Martin; Kariola, Tarja Johanna; Sipari, Nina Hannele; Pirhonen, Minna Ursula; Palva, Erkki Tapio.

In: BMC Plant Biology, Vol. 17, No. 19, 19, 19.01.2017.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Short oligogalacturonides induce pathogen resistance-associated gene expression in Arabidopsis thaliana

AU - Davidsson, Pär Roland

AU - Broberg, Martin

AU - Kariola, Tarja Johanna

AU - Sipari, Nina Hannele

AU - Pirhonen, Minna Ursula

AU - Palva, Erkki Tapio

PY - 2017/1/19

Y1 - 2017/1/19

N2 - BackgroundOligogalacturonides (OGs) are important components of damage-associated molecular pattern (DAMP) signaling and influence growth regulation in plants. Recent studies have focused on the impact of long OGs (degree of polymerization (DP) from 10–15), demonstrating the induction of plant defense signaling resulting in enhanced defenses to necrotrophic pathogens. To clarify the role of trimers (trimeric OGs, DP3) in DAMP signaling and their impact on plant growth regulation, we performed a transcriptomic analysis through the RNA sequencing of Arabidopsis thaliana exposed to trimers.ResultsThe transcriptomic data from trimer-treated Arabidopsis seedlings indicate a clear activation of genes involved in defense signaling, phytohormone signaling and a down-regulation of genes involved in processes related to growth regulation and development. This is further accompanied with improved defenses against necrotrophic pathogens triggered by the trimer treatment, indicating that short OGs have a clear impact on plant responses, similar to those described for long OGs.ConclusionsOur results demonstrate that trimers are indeed active elicitors of plant defenses. This is clearly indicated by the up-regulation of genes associated with plant defense signaling, accompanied with improved defenses against necrotrophic pathogens. Moreover, trimers simultaneously trigger a clear down-regulation of genes and gene sets associated with growth and development, leading to stunted seedling growth in Arabidopsis.KeywordsPlant signaling Arabidopsis thaliana Oligogalacturonides OG Trimers Transcriptomics Defense induction Growth inhibition Disease resistance Pectobacterium carotovorum Botrytis cinerea

AB - BackgroundOligogalacturonides (OGs) are important components of damage-associated molecular pattern (DAMP) signaling and influence growth regulation in plants. Recent studies have focused on the impact of long OGs (degree of polymerization (DP) from 10–15), demonstrating the induction of plant defense signaling resulting in enhanced defenses to necrotrophic pathogens. To clarify the role of trimers (trimeric OGs, DP3) in DAMP signaling and their impact on plant growth regulation, we performed a transcriptomic analysis through the RNA sequencing of Arabidopsis thaliana exposed to trimers.ResultsThe transcriptomic data from trimer-treated Arabidopsis seedlings indicate a clear activation of genes involved in defense signaling, phytohormone signaling and a down-regulation of genes involved in processes related to growth regulation and development. This is further accompanied with improved defenses against necrotrophic pathogens triggered by the trimer treatment, indicating that short OGs have a clear impact on plant responses, similar to those described for long OGs.ConclusionsOur results demonstrate that trimers are indeed active elicitors of plant defenses. This is clearly indicated by the up-regulation of genes associated with plant defense signaling, accompanied with improved defenses against necrotrophic pathogens. Moreover, trimers simultaneously trigger a clear down-regulation of genes and gene sets associated with growth and development, leading to stunted seedling growth in Arabidopsis.KeywordsPlant signaling Arabidopsis thaliana Oligogalacturonides OG Trimers Transcriptomics Defense induction Growth inhibition Disease resistance Pectobacterium carotovorum Botrytis cinerea

KW - 1183 Plant biology, microbiology, virology

U2 - 10.1186/s12870-016-0959-1

DO - 10.1186/s12870-016-0959-1

M3 - Article

VL - 17

JO - BMC Plant Biology

JF - BMC Plant Biology

SN - 1471-2229

IS - 19

M1 - 19

ER -