Soft tissue reactions to bioactive glass 13-93 combined with chitosan

Antti J Asikainen, J Hagström, Timo Sorsa, Jukka Noponen, Minna Kellomäki, Hanne Juuti, Christian Lindqvist, Jarkko Hietanen, Riitta Suuronen

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    The aim of this study was to evaluate rabbit soft tissue reactions to bioactive glass 13-93 mesh by using a histological and immunohistochemical analysis. Bioactive glass (13-93) mesh fixed with 3 wt % chitosan was implanted into the dorsal subcutaneous space of New Zealand White rabbits (n = 18) for six, 12, and 24 weeks, respectively. After 6 weeks the bioactive glass remnants were surrounded by foreign-body granuloma with eosinophilic granulocytes. After 12 and 24 weeks the implanted material was mainly absorbed, but, if any particles still remained the foreign-body reaction was notably milder. Yet, a mild chronic inflammatory infiltrate was present. Matrix metalloproteinase (MMP) -2, -3, -13 and tissue inhibitory protein (TIMP-1 and -2) expressions were studied by immunohistochemistry. MMP-3, -13, TIMP-1, and -2 positivity were detected throughout the follow-up period. MMP-2 positivity was only occasionally seen in the 24 week samples, which is constitutively expressed but is not related to inductive MMP-3 and -13 cascade. The presence of eosinophilic granulocytes in some of the samples raises the possibility of an allergic reaction to the materials. MMP-3 and -13 are suggested to participate in the host reaction to either bioactive glass or chitosan. (c) 2007 Wiley Periodicals, Inc.
    Original languageEnglish
    JournalJournal of Biomedical Materials Research. Part A
    Issue number2
    Pages (from-to)530-537
    Publication statusPublished - 2007
    MoE publication typeA1 Journal article-refereed

    Fields of Science

    • 311 Basic medicine
    • 312 Clinical medicine
    • 313 Dentistry

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