Abstract

The majority of nitrogen (N) in boreal forest soils is bound to soil organic matter (SOM) in forms not readily available to plants. Northern boreal forest ecosystems are often N limited, despite atmospheric N deposition, and the utilization of organic N from SOM is of crucial importance to the site productivity. The effect of microbial produced oxidative SOM degrading enzymes (laccase and manganese peroxidases) and proteases on soil N forms and availability was studied in a pot experiment with or without a Scots pine (Pinus sylvestris L.) seedling. The combination of SOM degrading enzymes and proteases decreased the total soil N content and increased the N losses significantly in the absence of the Scots pine seedlings. The total soil N content also decreased in the presence of the Scots pine seedlings, irrespective of the enzyme treatment. Most of the other N parameters studied were not sensitive to enzyme additions, and differed only between planted and non-planted treatments. Our results show that the alkyl amine content of boreal forest soil are at the same levels as nitrate. We showed that SOM decomposition, stimulated by oxidative enzyme additions, is a key step in soil organic N utilization, while proteases alone do not increase N use from SOM. Plants stimulate N losses from SOM highlighting the importance of rhizosphere processes in soil C and N cycling. (C) 2016 Elsevier Ltd. All rights reserved.
Original languageEnglish
JournalSoil Biology & Biochemistry
Volume96
Pages (from-to)97-106
Number of pages10
ISSN0038-0717
DOIs
Publication statusPublished - 2016
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 114 Physical sciences
  • 116 Chemical sciences
  • 1172 Environmental sciences
  • 4112 Forestry
  • 416 Food Science

Cite this

@article{60cb24f930ee4eaeb762977063f4c78c,
title = "Stimulation of soil organic nitrogen pool: The effect of plant and soil organic matter degrading enzymes",
abstract = "The majority of nitrogen (N) in boreal forest soils is bound to soil organic matter (SOM) in forms not readily available to plants. Northern boreal forest ecosystems are often N limited, despite atmospheric N deposition, and the utilization of organic N from SOM is of crucial importance to the site productivity. The effect of microbial produced oxidative SOM degrading enzymes (laccase and manganese peroxidases) and proteases on soil N forms and availability was studied in a pot experiment with or without a Scots pine (Pinus sylvestris L.) seedling. The combination of SOM degrading enzymes and proteases decreased the total soil N content and increased the N losses significantly in the absence of the Scots pine seedlings. The total soil N content also decreased in the presence of the Scots pine seedlings, irrespective of the enzyme treatment. Most of the other N parameters studied were not sensitive to enzyme additions, and differed only between planted and non-planted treatments. Our results show that the alkyl amine content of boreal forest soil are at the same levels as nitrate. We showed that SOM decomposition, stimulated by oxidative enzyme additions, is a key step in soil organic N utilization, while proteases alone do not increase N use from SOM. Plants stimulate N losses from SOM highlighting the importance of rhizosphere processes in soil C and N cycling. (C) 2016 Elsevier Ltd. All rights reserved.",
keywords = "114 Physical sciences, 116 Chemical sciences, 1172 Environmental sciences, 4112 Forestry, 416 Food Science",
author = "Kieloaho, {Antti-Jussi Sakari} and Pihlatie, {Mari Katriina} and {Dominguez Carrasco}, {Maria del Rosario} and Kanerva, {Sanna Mari} and Evgeny Parshintsev and Marja-Liisa Riekkola and Pumpanen, {Jukka Sakari} and Heinonsalo, {Eero Jussi}",
year = "2016",
doi = "10.1016/j.soilbio.2016.01.013",
language = "English",
volume = "96",
pages = "97--106",
journal = "Soil Biology & Biochemistry",
issn = "0038-0717",
publisher = "Elsevier Scientific Publ. Co",

}

TY - JOUR

T1 - Stimulation of soil organic nitrogen pool

T2 - The effect of plant and soil organic matter degrading enzymes

AU - Kieloaho, Antti-Jussi Sakari

AU - Pihlatie, Mari Katriina

AU - Dominguez Carrasco, Maria del Rosario

AU - Kanerva, Sanna Mari

AU - Parshintsev, Evgeny

AU - Riekkola, Marja-Liisa

AU - Pumpanen, Jukka Sakari

AU - Heinonsalo, Eero Jussi

PY - 2016

Y1 - 2016

N2 - The majority of nitrogen (N) in boreal forest soils is bound to soil organic matter (SOM) in forms not readily available to plants. Northern boreal forest ecosystems are often N limited, despite atmospheric N deposition, and the utilization of organic N from SOM is of crucial importance to the site productivity. The effect of microbial produced oxidative SOM degrading enzymes (laccase and manganese peroxidases) and proteases on soil N forms and availability was studied in a pot experiment with or without a Scots pine (Pinus sylvestris L.) seedling. The combination of SOM degrading enzymes and proteases decreased the total soil N content and increased the N losses significantly in the absence of the Scots pine seedlings. The total soil N content also decreased in the presence of the Scots pine seedlings, irrespective of the enzyme treatment. Most of the other N parameters studied were not sensitive to enzyme additions, and differed only between planted and non-planted treatments. Our results show that the alkyl amine content of boreal forest soil are at the same levels as nitrate. We showed that SOM decomposition, stimulated by oxidative enzyme additions, is a key step in soil organic N utilization, while proteases alone do not increase N use from SOM. Plants stimulate N losses from SOM highlighting the importance of rhizosphere processes in soil C and N cycling. (C) 2016 Elsevier Ltd. All rights reserved.

AB - The majority of nitrogen (N) in boreal forest soils is bound to soil organic matter (SOM) in forms not readily available to plants. Northern boreal forest ecosystems are often N limited, despite atmospheric N deposition, and the utilization of organic N from SOM is of crucial importance to the site productivity. The effect of microbial produced oxidative SOM degrading enzymes (laccase and manganese peroxidases) and proteases on soil N forms and availability was studied in a pot experiment with or without a Scots pine (Pinus sylvestris L.) seedling. The combination of SOM degrading enzymes and proteases decreased the total soil N content and increased the N losses significantly in the absence of the Scots pine seedlings. The total soil N content also decreased in the presence of the Scots pine seedlings, irrespective of the enzyme treatment. Most of the other N parameters studied were not sensitive to enzyme additions, and differed only between planted and non-planted treatments. Our results show that the alkyl amine content of boreal forest soil are at the same levels as nitrate. We showed that SOM decomposition, stimulated by oxidative enzyme additions, is a key step in soil organic N utilization, while proteases alone do not increase N use from SOM. Plants stimulate N losses from SOM highlighting the importance of rhizosphere processes in soil C and N cycling. (C) 2016 Elsevier Ltd. All rights reserved.

KW - 114 Physical sciences

KW - 116 Chemical sciences

KW - 1172 Environmental sciences

KW - 4112 Forestry

KW - 416 Food Science

U2 - 10.1016/j.soilbio.2016.01.013

DO - 10.1016/j.soilbio.2016.01.013

M3 - Article

VL - 96

SP - 97

EP - 106

JO - Soil Biology & Biochemistry

JF - Soil Biology & Biochemistry

SN - 0038-0717

ER -