Storage and retrieval of individual genomes

Veli Mäkinen, Gonzalo Navarro, Jouni Sirén, Niko Välimäki

Research output: Chapter in Book/Report/Conference proceedingConference contributionScientificpeer-review

Abstract

A repetitive sequence collection is one where portions of a base sequence of length n are repeated many times with small variations, forming a collection of total length N. Examples of such collections are version control data and genome sequences of individuals, where the differences can be expressed by lists of basic edit operations. Flexible and efficient data analysis on a such typically huge collection is plausible using suffix trees. However, suffix tree occupies O(N log N) bits, which very soon inhibits in-memory analyses. Recent advances in full-text self-indexing reduce the space of suffix tree to O(N log σ) bits, where σ is the alphabet size. In practice, the space reduction is more than 10-fold, for example on suffix tree of Human Genome. However, this reduction factor remains constant when more sequences are added to the collection.

We develop a new family of self-indexes suited for the repetitive sequence collection setting. Their expected space requirement depends only on the length n of the base sequence and the number s of variations in its repeated copies. That is, the space reduction factor is no longer constant, but depends on N / n.

We believe the structures developed in this work will provide a fundamental basis for storage and retrieval of individual genomes as they become available due to rapid progress in the sequencing technologies.
Original languageEnglish
Title of host publicationResearch in Computational Molecular Biology : 13th Annual International Conference, RECOMB 2009
EditorsSerafim Batzoglou
Number of pages17
PublisherSpringer
Publication date2009
Pages121-137
ISBN (Print)978-3-642-02007-0
DOIs
Publication statusPublished - 2009
MoE publication typeA4 Article in conference proceedings
EventAnnual International Conference on Research in Computational Molecular Biology - Tucson, Arizona, United States
Duration: 18 May 200921 May 2009
Conference number: 13

Publication series

NameLecture Notes in Computer Science
Number5541

Fields of Science

  • 113 Computer and information sciences

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