Structural characterization of water-insoluble nonstarchy polysaccharides of oats and barley

Liisa Virkki, Liisa Johansson, Maija Ylinen, Sirkka Liisa Maunu, Päivi Ekholm

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Water-insoluble dietary fibre of dehulled oat and barley grain was isolated with enzymatic hydrolysis of starch and protein and further extracted sequentially. The extractants used were saturated barium hydroxide, water, 1 M potassium hydroxide and 4 M sodium hydroxide. The fractions were dialysed against pure water, freeze-dried and weighed. Repeatable recoveries of extracts were obtained. The ground grains and extracted fractions were characterized for their fat and protein content. The fractions were hydrolysed and characterized by measuring their neutral sugar composition using gas chromatography. Comparison of the amounts of cellulosic and noncellulosic polysaccharides was made. Further characterization of fractions was performed with solid-state C-13 CP/MAS NMR spectroscopy and IR spectroscopy. The differences in composition of insoluble material between oats and barley were small. The distribution of masses and monosaccharides of the five fractions obtained were similar and the measured spectra show the same characteristic signals as saccharide structures. (C) 2004 Elsevier Ltd. All rights reserved.
Original languageEnglish
JournalCarbohydrate Polymers
Volume59
Issue number3
Pages (from-to)357-366
Number of pages10
ISSN0144-8617
DOIs
Publication statusPublished - 2005
MoE publication typeA1 Journal article-refereed

Cite this

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title = "Structural characterization of water-insoluble nonstarchy polysaccharides of oats and barley",
abstract = "Water-insoluble dietary fibre of dehulled oat and barley grain was isolated with enzymatic hydrolysis of starch and protein and further extracted sequentially. The extractants used were saturated barium hydroxide, water, 1 M potassium hydroxide and 4 M sodium hydroxide. The fractions were dialysed against pure water, freeze-dried and weighed. Repeatable recoveries of extracts were obtained. The ground grains and extracted fractions were characterized for their fat and protein content. The fractions were hydrolysed and characterized by measuring their neutral sugar composition using gas chromatography. Comparison of the amounts of cellulosic and noncellulosic polysaccharides was made. Further characterization of fractions was performed with solid-state C-13 CP/MAS NMR spectroscopy and IR spectroscopy. The differences in composition of insoluble material between oats and barley were small. The distribution of masses and monosaccharides of the five fractions obtained were similar and the measured spectra show the same characteristic signals as saccharide structures. (C) 2004 Elsevier Ltd. All rights reserved.",
author = "Liisa Virkki and Liisa Johansson and Maija Ylinen and Maunu, {Sirkka Liisa} and P{\"a}ivi Ekholm",
year = "2005",
doi = "10.1016/j.carbpol.2004.10.006",
language = "English",
volume = "59",
pages = "357--366",
journal = "Carbohydrate Polymers",
issn = "0144-8617",
publisher = "ELSEVIER SCI IRELAND LTD",
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}

Structural characterization of water-insoluble nonstarchy polysaccharides of oats and barley. / Virkki, Liisa; Johansson, Liisa; Ylinen, Maija; Maunu, Sirkka Liisa; Ekholm, Päivi.

In: Carbohydrate Polymers, Vol. 59, No. 3, 2005, p. 357-366.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Structural characterization of water-insoluble nonstarchy polysaccharides of oats and barley

AU - Virkki, Liisa

AU - Johansson, Liisa

AU - Ylinen, Maija

AU - Maunu, Sirkka Liisa

AU - Ekholm, Päivi

PY - 2005

Y1 - 2005

N2 - Water-insoluble dietary fibre of dehulled oat and barley grain was isolated with enzymatic hydrolysis of starch and protein and further extracted sequentially. The extractants used were saturated barium hydroxide, water, 1 M potassium hydroxide and 4 M sodium hydroxide. The fractions were dialysed against pure water, freeze-dried and weighed. Repeatable recoveries of extracts were obtained. The ground grains and extracted fractions were characterized for their fat and protein content. The fractions were hydrolysed and characterized by measuring their neutral sugar composition using gas chromatography. Comparison of the amounts of cellulosic and noncellulosic polysaccharides was made. Further characterization of fractions was performed with solid-state C-13 CP/MAS NMR spectroscopy and IR spectroscopy. The differences in composition of insoluble material between oats and barley were small. The distribution of masses and monosaccharides of the five fractions obtained were similar and the measured spectra show the same characteristic signals as saccharide structures. (C) 2004 Elsevier Ltd. All rights reserved.

AB - Water-insoluble dietary fibre of dehulled oat and barley grain was isolated with enzymatic hydrolysis of starch and protein and further extracted sequentially. The extractants used were saturated barium hydroxide, water, 1 M potassium hydroxide and 4 M sodium hydroxide. The fractions were dialysed against pure water, freeze-dried and weighed. Repeatable recoveries of extracts were obtained. The ground grains and extracted fractions were characterized for their fat and protein content. The fractions were hydrolysed and characterized by measuring their neutral sugar composition using gas chromatography. Comparison of the amounts of cellulosic and noncellulosic polysaccharides was made. Further characterization of fractions was performed with solid-state C-13 CP/MAS NMR spectroscopy and IR spectroscopy. The differences in composition of insoluble material between oats and barley were small. The distribution of masses and monosaccharides of the five fractions obtained were similar and the measured spectra show the same characteristic signals as saccharide structures. (C) 2004 Elsevier Ltd. All rights reserved.

U2 - 10.1016/j.carbpol.2004.10.006

DO - 10.1016/j.carbpol.2004.10.006

M3 - Article

VL - 59

SP - 357

EP - 366

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

SN - 0144-8617

IS - 3

ER -