Structural characterization of water-soluble β-glucan of oat bran

L. Johansson, L. Virkki, S. Maunu, M. Lehto, P. Ekholm, P. Varo

Research output: Contribution to journalArticleScientificpeer-review

Abstract

β-Glucan was isolated from oat bran in a highly purified form. The bran was characterized for its contents of dietary fiber, β-glucan, fat and protein. The isolated β-glucan was free of protein and contained only glucose in GC sugar anal. Two types of β-glucan were obtained with different solubilities. Their molar masses were 1.6 million for the less sol. and 1.1 million for the more readily sol. type. No structural differences were found. The two-dimensional correlation NMR spectrum of the isolated β-glucan showed that the glucose units are joined with 1,3- and 1,4-linkages only. The oligosaccharides produced by the action of a specific enzyme, lichenase, were analyzed by HPLC and capillary zone electrophoresis. The major products are 32-β-D-glucosyl cellobiose (trisaccharide) and 33-β-D-glucosyl cellotriose (tetrasaccharide), which account for 95% of the whole. Also, 34-β-D-glucosyl cellotetraose (pentasaccharide) and other oligosaccharides with d.p. (DP) higher than 5 were detected as minor components. [on SciFinder(R)]
Original languageEnglish
JournalCarbohydrate Polymers
Volume42
Issue number2
Pages (from-to)143-148
Number of pages6
ISSN0144-8617
DOIs
Publication statusPublished - 2000
MoE publication typeA1 Journal article-refereed

Fields of Science

  • oat bran glucan structure oligosaccharide

Cite this

Johansson, L. ; Virkki, L. ; Maunu, S. ; Lehto, M. ; Ekholm, P. ; Varo, P. / Structural characterization of water-soluble β-glucan of oat bran. In: Carbohydrate Polymers. 2000 ; Vol. 42, No. 2. pp. 143-148.
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abstract = "β-Glucan was isolated from oat bran in a highly purified form. The bran was characterized for its contents of dietary fiber, β-glucan, fat and protein. The isolated β-glucan was free of protein and contained only glucose in GC sugar anal. Two types of β-glucan were obtained with different solubilities. Their molar masses were 1.6 million for the less sol. and 1.1 million for the more readily sol. type. No structural differences were found. The two-dimensional correlation NMR spectrum of the isolated β-glucan showed that the glucose units are joined with 1,3- and 1,4-linkages only. The oligosaccharides produced by the action of a specific enzyme, lichenase, were analyzed by HPLC and capillary zone electrophoresis. The major products are 32-β-D-glucosyl cellobiose (trisaccharide) and 33-β-D-glucosyl cellotriose (tetrasaccharide), which account for 95{\%} of the whole. Also, 34-β-D-glucosyl cellotetraose (pentasaccharide) and other oligosaccharides with d.p. (DP) higher than 5 were detected as minor components. [on SciFinder(R)]",
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Structural characterization of water-soluble β-glucan of oat bran. / Johansson, L.; Virkki, L.; Maunu, S.; Lehto, M.; Ekholm, P.; Varo, P.

In: Carbohydrate Polymers, Vol. 42, No. 2, 2000, p. 143-148.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Structural characterization of water-soluble β-glucan of oat bran

AU - Johansson, L.

AU - Virkki, L.

AU - Maunu, S.

AU - Lehto, M.

AU - Ekholm, P.

AU - Varo, P.

N1 - M1 - Copyright (C) 2011 American Chemical Society (ACS). All Rights Reserved. CAPLUS AN 2000:207706(Journal) Volume: Proceeding volume:

PY - 2000

Y1 - 2000

N2 - β-Glucan was isolated from oat bran in a highly purified form. The bran was characterized for its contents of dietary fiber, β-glucan, fat and protein. The isolated β-glucan was free of protein and contained only glucose in GC sugar anal. Two types of β-glucan were obtained with different solubilities. Their molar masses were 1.6 million for the less sol. and 1.1 million for the more readily sol. type. No structural differences were found. The two-dimensional correlation NMR spectrum of the isolated β-glucan showed that the glucose units are joined with 1,3- and 1,4-linkages only. The oligosaccharides produced by the action of a specific enzyme, lichenase, were analyzed by HPLC and capillary zone electrophoresis. The major products are 32-β-D-glucosyl cellobiose (trisaccharide) and 33-β-D-glucosyl cellotriose (tetrasaccharide), which account for 95% of the whole. Also, 34-β-D-glucosyl cellotetraose (pentasaccharide) and other oligosaccharides with d.p. (DP) higher than 5 were detected as minor components. [on SciFinder(R)]

AB - β-Glucan was isolated from oat bran in a highly purified form. The bran was characterized for its contents of dietary fiber, β-glucan, fat and protein. The isolated β-glucan was free of protein and contained only glucose in GC sugar anal. Two types of β-glucan were obtained with different solubilities. Their molar masses were 1.6 million for the less sol. and 1.1 million for the more readily sol. type. No structural differences were found. The two-dimensional correlation NMR spectrum of the isolated β-glucan showed that the glucose units are joined with 1,3- and 1,4-linkages only. The oligosaccharides produced by the action of a specific enzyme, lichenase, were analyzed by HPLC and capillary zone electrophoresis. The major products are 32-β-D-glucosyl cellobiose (trisaccharide) and 33-β-D-glucosyl cellotriose (tetrasaccharide), which account for 95% of the whole. Also, 34-β-D-glucosyl cellotetraose (pentasaccharide) and other oligosaccharides with d.p. (DP) higher than 5 were detected as minor components. [on SciFinder(R)]

KW - oat bran glucan structure oligosaccharide

U2 - 10.1016/S0144-8617(99)00157-5

DO - 10.1016/S0144-8617(99)00157-5

M3 - Article

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JO - Carbohydrate Polymers

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