Abstract
Sulphite addition during steam pretreatment of softwoods under acidic, neutral and alkaline conditions was assessed to try to minimize lignin condensation. Although pretreatment under neutral/alkaline conditions resulted in effective lignin sulphonation, non-uniform size reduction was observed. In contrast, acidic sulphite steam treatment at 210 °C for 10 min resulted in homogenous particle size reduction and water-insoluble component that was 62% carbohydrate and 33% lignin. This carbohydrate-rich substrate was readily hydrolyzed and fermented which indicated the lack of fermentation inhibitors in the steam-pretreated whole slurry. The use of high solid loading (25% w/v) resulted in a hydrolysis yield of 58% at an enzyme loading of 40 mg protein/g glucan and efficient fermentation (46.6 g/L of ethanol). This indicated that the addition of acidic sulphite at the steam pretreatment of softwoods improved both the enzymatic hydrolysis and fermentation of steam-pretreated whole slurries. Graphical Abstract: [Figure not available: see fulltext.]
Original language | English |
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Article number | 71 |
Journal | Bioresources and bioprocessing |
Volume | 9 |
Issue number | 1 |
Number of pages | 9 |
ISSN | 2197-4365 |
DOIs | |
Publication status | Published - Dec 2022 |
MoE publication type | A1 Journal article-refereed |
Bibliographical note
Publisher Copyright:© 2022, The Author(s).
Fields of Science
- Acid steam pretreatment
- Detoxification
- Fermentation
- High consistency hydrolysis
- Sulphite steam pretreatment
- Whole slurry
- 116 Chemical sciences