Targeted metabolomics with Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS) highlights metabolic differences in Healthy and Atopic Staffordshire Bull terriers after two different diets

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Emerging evidence has indicated that a raw meat-based diet may lower the overall risk of common chronic systemic and metabolic diseases in canines. Canine atopic dermatitis (CAD), a common chronic disease, has also been linked to metabolic health but its relation to diet remains poorly understood. This study aimed to provide quantitative evidence for how dietary choice is linked to metabolic changes in healthy and CAD-diagnosed canines via targeted serum and urine metabolomic analysis of polar, non-ionic compounds. The response to diets were analysed with a targeted metabolomics profile directly related to nutritional intake, hence yielding information on metabolic health, as well as to whether the underlying CAD-condition may modulate the response to nutritional intake. Two cohorts of pet Staffordshire Bull Terriers, one with only CAD diagnosed canines (n=8), and one with both CAD (n= 6) and healthy canines (n= 6) were studied using targeted serum and urine metabolomic analysis. Both cohorts were further split into two groups based on diet, and were fed either a commercially available minimally processed, raw meat based diet (RMBD) (ntotal=11), or a commercially available ultra-processed, kibble diet (KD) (ntotal=9) over a diet intervention period lasting approx. 4.5 months (median 140d). Analysis of the serum metabolite profiles across both batches (n=20), and of the urine metabolite of the CAD-diagnosed cohort (n=8) was performed. Metabolite data was interpreted using various statistical models as well as metabolic pathway analysis. Our results indicate that significant differences in metabolite concentrations of the one-carbon pathway were detected in response to diet in serum (combined cohorts, p<0.001, FDR ≤ 0.05) and urine (CAD-only cohort, p<0.001, FDR ≤ 0.05). These play important roles in both homocysteine metabolism and s-adenosyl methionine (SAM) production, and thus have roles in detoxification of harmful compounds and immune function, primarily through regulatory processes of gene expression. When comparing the CAD + healthy cohort to only CAD diagnosed individuals, results suggest that health status may have had an impact on the outcome. The response in the fully atopic cohort (Batch 1, n=8), as well as CAD-diagnosed individuals from the mixed-health status cohort (Batch 2, n=6), showed starker metabolic changes in response to diet regarding these pathways compared to healthy individuals (Batch 2, n=6). This may suggest that the underlying CAD condition may disrupt normal regulation of these metabolites in response to diet, leading to starker differences in metabolic activity in the CAD-diagnosed individuals eating either diet. In this context, our results indicate that dogs fed a RMBD showed less activity in processes involved with extrahepatic homocysteine clearance, as well as SAM production than in the KD diet. Our preliminary findings provide insight into the relationship between diet and the CAD condition on a metabolic level.
Original languageEnglish
JournalFrontiers in Veterinary Science
Publication statusSubmitted - 15 Mar 2020
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 3143 Nutrition
  • 413 Veterinary science
  • 318 Medical biotechnology

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