Taxonomic diversity and symbiotic efficiency of rhizobial strains obtained from nodules of Medicago sativa growing in Iran

Ali Soltani Toularoud, Aregu Amsalu Aserse, Hasein A. Alikhani, Gholam Reza Salehi Jouzani, Hadi Asadi Rahmani, Kazem Khavazi, Leena Annikki Räsänen, Anna Kristina Lindström

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Forty-eight rhizobial strains isolated from root nodules of alfalfa (Medicago sativa L.) plants growing in different regions of Iran were characterized by using restriction fragment length polymorphism (RFLP) of ribosomal internal transcribed spacer (ITS) region, 16S RNA gene-RFLP and sequence analysis of symbiotic genesnodA and nifH. Taxonomic relationships of selected strains from different ITS-RFLP groups were further inferred in this study by using single and concatenated phylogenetic analysis of recA and atpD gene sequences. In all constructed trees, the strains were distributed similarly into three different rhizobial phylogenetic groups I, II and III. Group I included strains belonging to Sinorhizobium (Ensifer)meliloti and S.kummerowiae. The strains in groupsII and III were identified as E. adhaerens and Agrobacterium radiobacter, respectively. Based on the two core genes used in this study, S.kummerowiaestrains appeared to be very closely related to S. melilotireference strains. Forty-two strains induced nitrogen-fixing nodules on alfalfa plants. Remarkably, two of these strains belonged to A. radiobacter. The entire three test strains in group II (E. adhaerens) and one test strain in group III (A. radiobacter) failed to nodulate. Generally, our results confirm that S. meliloti is the most important symbionts of M. sativa growing in agricultural fields in Iran and question S. kummerowiae being a species separate from S. meliloti.
Original languageEnglish
JournalAnnals of Biological research
Volume7
Issue number3
Pages (from-to)29-38
Number of pages10
ISSN0976-1233
Publication statusPublished - Mar 2016
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 1172 Environmental sciences

Cite this

Toularoud, Ali Soltani ; Aserse, Aregu Amsalu ; Alikhani, Hasein A. ; Jouzani, Gholam Reza Salehi ; Rahmani, Hadi Asadi ; Khavazi, Kazem ; Räsänen, Leena Annikki ; Lindström, Anna Kristina. / Taxonomic diversity and symbiotic efficiency of rhizobial strains obtained from nodules of Medicago sativa growing in Iran. In: Annals of Biological research. 2016 ; Vol. 7, No. 3. pp. 29-38.
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title = "Taxonomic diversity and symbiotic efficiency of rhizobial strains obtained from nodules of Medicago sativa growing in Iran",
abstract = "Forty-eight rhizobial strains isolated from root nodules of alfalfa (Medicago sativa L.) plants growing in different regions of Iran were characterized by using restriction fragment length polymorphism (RFLP) of ribosomal internal transcribed spacer (ITS) region, 16S RNA gene-RFLP and sequence analysis of symbiotic genesnodA and nifH. Taxonomic relationships of selected strains from different ITS-RFLP groups were further inferred in this study by using single and concatenated phylogenetic analysis of recA and atpD gene sequences. In all constructed trees, the strains were distributed similarly into three different rhizobial phylogenetic groups I, II and III. Group I included strains belonging to Sinorhizobium (Ensifer)meliloti and S.kummerowiae. The strains in groupsII and III were identified as E. adhaerens and Agrobacterium radiobacter, respectively. Based on the two core genes used in this study, S.kummerowiaestrains appeared to be very closely related to S. melilotireference strains. Forty-two strains induced nitrogen-fixing nodules on alfalfa plants. Remarkably, two of these strains belonged to A. radiobacter. The entire three test strains in group II (E. adhaerens) and one test strain in group III (A. radiobacter) failed to nodulate. Generally, our results confirm that S. meliloti is the most important symbionts of M. sativa growing in agricultural fields in Iran and question S. kummerowiae being a species separate from S. meliloti.",
keywords = "1172 Environmental sciences",
author = "Toularoud, {Ali Soltani} and Aserse, {Aregu Amsalu} and Alikhani, {Hasein A.} and Jouzani, {Gholam Reza Salehi} and Rahmani, {Hadi Asadi} and Kazem Khavazi and R{\"a}s{\"a}nen, {Leena Annikki} and Lindstr{\"o}m, {Anna Kristina}",
year = "2016",
month = "3",
language = "English",
volume = "7",
pages = "29--38",
journal = "Annals of Biological research",
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Taxonomic diversity and symbiotic efficiency of rhizobial strains obtained from nodules of Medicago sativa growing in Iran. / Toularoud, Ali Soltani; Aserse, Aregu Amsalu; Alikhani, Hasein A.; Jouzani, Gholam Reza Salehi; Rahmani, Hadi Asadi; Khavazi, Kazem; Räsänen, Leena Annikki; Lindström, Anna Kristina.

In: Annals of Biological research, Vol. 7, No. 3, 03.2016, p. 29-38.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Taxonomic diversity and symbiotic efficiency of rhizobial strains obtained from nodules of Medicago sativa growing in Iran

AU - Toularoud, Ali Soltani

AU - Aserse, Aregu Amsalu

AU - Alikhani, Hasein A.

AU - Jouzani, Gholam Reza Salehi

AU - Rahmani, Hadi Asadi

AU - Khavazi, Kazem

AU - Räsänen, Leena Annikki

AU - Lindström, Anna Kristina

PY - 2016/3

Y1 - 2016/3

N2 - Forty-eight rhizobial strains isolated from root nodules of alfalfa (Medicago sativa L.) plants growing in different regions of Iran were characterized by using restriction fragment length polymorphism (RFLP) of ribosomal internal transcribed spacer (ITS) region, 16S RNA gene-RFLP and sequence analysis of symbiotic genesnodA and nifH. Taxonomic relationships of selected strains from different ITS-RFLP groups were further inferred in this study by using single and concatenated phylogenetic analysis of recA and atpD gene sequences. In all constructed trees, the strains were distributed similarly into three different rhizobial phylogenetic groups I, II and III. Group I included strains belonging to Sinorhizobium (Ensifer)meliloti and S.kummerowiae. The strains in groupsII and III were identified as E. adhaerens and Agrobacterium radiobacter, respectively. Based on the two core genes used in this study, S.kummerowiaestrains appeared to be very closely related to S. melilotireference strains. Forty-two strains induced nitrogen-fixing nodules on alfalfa plants. Remarkably, two of these strains belonged to A. radiobacter. The entire three test strains in group II (E. adhaerens) and one test strain in group III (A. radiobacter) failed to nodulate. Generally, our results confirm that S. meliloti is the most important symbionts of M. sativa growing in agricultural fields in Iran and question S. kummerowiae being a species separate from S. meliloti.

AB - Forty-eight rhizobial strains isolated from root nodules of alfalfa (Medicago sativa L.) plants growing in different regions of Iran were characterized by using restriction fragment length polymorphism (RFLP) of ribosomal internal transcribed spacer (ITS) region, 16S RNA gene-RFLP and sequence analysis of symbiotic genesnodA and nifH. Taxonomic relationships of selected strains from different ITS-RFLP groups were further inferred in this study by using single and concatenated phylogenetic analysis of recA and atpD gene sequences. In all constructed trees, the strains were distributed similarly into three different rhizobial phylogenetic groups I, II and III. Group I included strains belonging to Sinorhizobium (Ensifer)meliloti and S.kummerowiae. The strains in groupsII and III were identified as E. adhaerens and Agrobacterium radiobacter, respectively. Based on the two core genes used in this study, S.kummerowiaestrains appeared to be very closely related to S. melilotireference strains. Forty-two strains induced nitrogen-fixing nodules on alfalfa plants. Remarkably, two of these strains belonged to A. radiobacter. The entire three test strains in group II (E. adhaerens) and one test strain in group III (A. radiobacter) failed to nodulate. Generally, our results confirm that S. meliloti is the most important symbionts of M. sativa growing in agricultural fields in Iran and question S. kummerowiae being a species separate from S. meliloti.

KW - 1172 Environmental sciences

M3 - Article

VL - 7

SP - 29

EP - 38

JO - Annals of Biological research

JF - Annals of Biological research

SN - 0976-1233

IS - 3

ER -