Projects per year
Abstract
The basidiomycete white-rot fungus Obba rivulosa, a close relative of Gelatoporia (Ceriporiopsis) subvermispora, is an efficient degrader of softwood. The dikaryotic O. rivulosa strain T241i (FBCC949) has been shown to selectively remove lignin from spruce wood prior to depolymerization of plant cell wall polysaccharides, thus possessing potential in biotechnological applications such as pretreatment of wood in pulp and paper industry. In this work, we studied the time-course of the conversion of spruce by the genome-sequenced monokaryotic O. rivulosa strain 3A-2, which is derived from the dikaryon T241i, to get insight into transcriptome level changes during prolonged solid state cultivation. During 8-week cultivation, O. rivulosa expressed a constitutive set of genes encoding putative plant cell wall degrading enzymes. High level of expression of the genes targeted towards all plant cell wall polymers was detected at 2-week time point, after which majority of the genes showed reduced expression. This implicated non-selective degradation of lignin by the O. rivulosa monokaryon and suggests high variation between mono- and dikaryotic strains of the white-rot fungi with respect to their abilities to convert plant cell wall polymers.
Original language | English |
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Journal | Fungal Genetics and Biology |
Volume | 112 |
Pages (from-to) | 47-54 |
Number of pages | 8 |
ISSN | 1087-1845 |
DOIs | |
Publication status | Published - Mar 2018 |
MoE publication type | A1 Journal article-refereed |
Fields of Science
- 1184 Genetics, developmental biology, physiology
- Obba rivulosa
- White-rot
- Lignocellulose
- Solid state cultivation
- Transcriptomics
- CERIPORIOPSIS-SUBVERMISPORA
- PHANEROCHAETE-CHRYSOSPORIUM
- PHYSISPORINUS-RIVULOSUS
- OXALIC-ACID
- MANGANESE PEROXIDASES
- OXALATE DECARBOXYLASE
- DICHOMITUS-SQUALENS
- SECRETOME ANALYSIS
- POSTIA-PLACENTA
- ENZYMES
- Obba rivulosa
- White-rot
- Lignocellulose
- Solid state cultivation
- Transcriptomics
- CERIPORIOPSIS-SUBVERMISPORA
- PHANEROCHAETE-CHRYSOSPORIUM
- PHYSISPORINUS-RIVULOSUS
- OXALIC-ACID
- MANGANESE PEROXIDASES
- OXALATE DECARBOXYLASE
- DICHOMITUS-SQUALENS
- SECRETOME ANALYSIS
- POSTIA-PLACENTA
- ENZYMES
Projects
- 1 Finished
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Genome of the polypore Obba rivulosa (Gelatoporiaceae, Polyporales)
Miettinen, O. (Other), Hilden, K. (Participant), Mäkelä, M. (Participant), Hibbett, D. (Participant), Spatafora, J. (Participant), Grigoriev, I. (Participant), Barry, K. (Participant) & Hatakka, A. (Participant)
01/10/2013 → 31/12/2018
Project: Research project