The alteration of lipidomic profiles correlates with gene expression and physiological parameters of dairy cows during the periparturient period

Nanbing Qin, Shaimaa Selim, Tuulikki Seppänen-Laakso, Mika Hilvo, Juhani Taponen, Siru Salin, Aila Vanhatalo, Kari Elo, Tuomo Kokkonen

Research output: Chapter in Book/Report/Conference proceedingConference contributionScientificpeer-review

Abstract

Dairy cows undergo physiological adaptions during the periparturient period, including adipose tissue mobilization and the alteration of transcriptional factors in the liver and adipose tissue. During this period, overfeeding may alter lipidomic profiles in the peripheral tissues and subsequently influence the lipid and glucose metabolisms via the regulation of insulin action. Our aim was to study the interactions between the lipidomic and gene expression profiles and physiological parameters of dairy cows during the periparturient period. The experimental animals consisted of 16 multiparous cows in two groups. Controlled-energy feeding (100% of energy requirement of pregnant dairy cows) and high-energy feeding (142% of energy requirement of pregnant dairy cows) were applied to each group. The lipids in the subcutaneous adipose tissue and liver were identified and quantified using liquid chromatography-mass spectrometry based lipidomics from the samples collected at 8 days prepartum and 9 days postpartum. The identified lipid classes included ceramide (Cer), triglyceride (TG), diacylglycerol (DG), phosphatidylcholines (PC), phosphatidyl ethanolamine (PE), and sphingomyelin (SM). Spearman correlation analyses were conducted between lipids concentration, and the expression level of key factors in energy metabolism and physiological measurements.In the adipose tissue, the prepartal expression of insulin receptor 1 (IRS1) was negatively correlated with Cer (P<0.05) but positively correlated with TG (P<0.05). In the liver, prepartal glucose transporter type 4 (GLUT4) expression was negatively correlated with Cer (P<0.05) and SM (P<0.05). Moreover, prepartal liver Cer had a tendency to negatively correlate (P<0.10) with mitochondrial carnitine palmitoyltransferase 1 (CPT1) expression. However, Cer presented no positive correlation with plasma non-esterified fatty acid (NEFA) concentration neither in liver nor adipose tissue, suggesting that liver Cer relates to hepatic fatty acid oxidation but not to liver fatty acid uptake. Interestingly, there was a tendency of positive correlations (P<0.10) between prepartal dry matter intake, energy balance, or metabolic energy intake with Cer, PC, and PE, though these lipids displayed no relationship with body fatness in that time period.The alteration of lipidomic profiles caused by overfeeding prepartum shows interactions with changes of expression profile of proteins involved in lipid metabolism and insulin signalling in dairy cows during the periparturient period.
Original languageEnglish
Title of host publicationProceedings of the first dairycare conference : Copenhagen, August 22nd and 23rd 2014
EditorsC. H. Knight
Number of pages1
Place of PublicationCopenhagen
PublisherDairyCare COST Action FA1308
Publication date2014
Pages57
ISBN (Electronic)978-0-9930176-0-5
Publication statusPublished - 2014
MoE publication typeA4 Article in conference proceedings
EventDairycare conference - Copenhagen, Denmark
Duration: 22 Aug 201423 Aug 2014
Conference number: 1st

Fields of Science

  • 1182 Biochemistry, cell and molecular biology

Cite this

Qin, N., Selim, S., Seppänen-Laakso, T., Hilvo, M., Taponen, J., Salin, S., Vanhatalo, A., Elo, K., & Kokkonen, T. (2014). The alteration of lipidomic profiles correlates with gene expression and physiological parameters of dairy cows during the periparturient period. In C. H. Knight (Ed.), Proceedings of the first dairycare conference: Copenhagen, August 22nd and 23rd 2014 (pp. 57). DairyCare COST Action FA1308.