The application of LTR retrotransposons as molecular markers in plants.

Alan Schulman, Andrew Flavell, Etienne Paux, T. H. Noel Ellis

Research output: Book/ReportAnthology or special issueScientificpeer-review

Abstract

Retrotransposons are a major agent of genome evolution. Various molecular marker systems have been developed that exploit the ubiquitous nature of these genetic elements and their property of stable integration into dispersed chromosomal loci that are polymorphic within species. The key methods, SSAP, IRAP, REMAP, RBIP, and ISBP, all detect the sites at which the retrotransposon DNA, which is conserved between families of elements, is integrated into the genome. Marker systems exploiting these methods can
be easily developed and inexpensively deployed in the absence of extensive genome sequence data. They offer access to the dynamic and polymorphic, nongenic portion of the genome and thereby complement methods, such as gene-derived SNPs, that target primarily the genic fraction.
Original languageEnglish
PublisherHumana Press
ISBN (Print)978-1-61779-602-9
ISBN (Electronic)978-1-61779-603-6
DOIs
Publication statusPublished - 2012
MoE publication typeC2 Edited book

Publication series

NameMethods in Molecular Biology
No.859
ISSN (Print)1064-3745

Fields of Science

  • 1182 Biochemistry, cell and molecular biology
  • 1183 Plant biology, microbiology, virology

Cite this

Schulman, A., Flavell, A., Paux, E., & Ellis, T. H. N. (2012). The application of LTR retrotransposons as molecular markers in plants. (Methods in Molecular Biology; No. 859). Humana Press. https://doi.org/10.1007/978-1-61779-603-6_7
Schulman, Alan ; Flavell, Andrew ; Paux, Etienne ; Ellis, T. H. Noel. / The application of LTR retrotransposons as molecular markers in plants. Humana Press, 2012. (Methods in Molecular Biology; 859).
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Schulman, A, Flavell, A, Paux, E & Ellis, THN 2012, The application of LTR retrotransposons as molecular markers in plants. Methods in Molecular Biology, no. 859, Humana Press. https://doi.org/10.1007/978-1-61779-603-6_7

The application of LTR retrotransposons as molecular markers in plants. / Schulman, Alan; Flavell, Andrew; Paux, Etienne; Ellis, T. H. Noel.

Humana Press, 2012. (Methods in Molecular Biology; No. 859).

Research output: Book/ReportAnthology or special issueScientificpeer-review

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AB - Retrotransposons are a major agent of genome evolution. Various molecular marker systems have been developed that exploit the ubiquitous nature of these genetic elements and their property of stable integration into dispersed chromosomal loci that are polymorphic within species. The key methods, SSAP, IRAP, REMAP, RBIP, and ISBP, all detect the sites at which the retrotransposon DNA, which is conserved between families of elements, is integrated into the genome. Marker systems exploiting these methods can be easily developed and inexpensively deployed in the absence of extensive genome sequence data. They offer access to the dynamic and polymorphic, nongenic portion of the genome and thereby complement methods, such as gene-derived SNPs, that target primarily the genic fraction.

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Schulman A, Flavell A, Paux E, Ellis THN. The application of LTR retrotransposons as molecular markers in plants. Humana Press, 2012. (Methods in Molecular Biology; 859). https://doi.org/10.1007/978-1-61779-603-6_7