The effect of lignin model compound structure on the rate of oxidation catalyzed by two different fungal laccases

Maarit Lahtinen, Kristiina Kruus, Harry Boer, Marianna Kemell, Martina Andberg, Liisa Viikari, Jussi Sipilä

    Research output: Contribution to journalArticleScientificpeer-review

    Abstract

    Laccases (EC 1.10.3.2) are multicopper oxidases able to oxidize various substrates, such as phenolic sub-units of lignin. The substrate range can be widened to non-phenolic units by the use of mediators. Since discovery of the laccase-mediator system, direct reactions of lignin and laccase without mediated electron-transfer have gained much less attention. The objective of this study was to investigate lignin as a substrate for fungal laccases by using lignin model compounds. These model compounds contained guaiacylic and syringylic moieties and also compounds of guaiacylic origin at a higher oxidation level. Some of these compounds are commercially available. but most of them were synthesized. The oxidation reaction rates of the lignin model compounds were studied by monitoring consumption of the co-substrate oxygen, in reactions catalyzed by laccases from two different fungi: Melanocarpus albomyces and Trametes hirsuta, possessing different molecular and catalytic properties. These reaction rate studies were compared to physicochemical properties of the lignin model compounds: relative redox potentials determined using cyclic voltammetry and pK(a)-values. Docking of syringylic and biphenylic compounds to the active sites of both laccases was performed and the resulting model complex structures were used to further interpret the reaction rate results. Reaction rates of laccases are mainly affected by the ability of a lignin model compound to be oxidized and the pK(a)-value of the substrate seems to be less important. As a consequence, syringylic compounds are oxidized with the highest rates and compounds at a higher oxidation level and redox-potential, such as vanillin, are oxidized at a much lower rate. Both guaiacylic and syringylic type compounds fit well in the active sites of both laccases. Only for a biphenylic compound, steric clashes were observed, and they are likely to have an effect on the reaction rate. When the oxidation rates on the selected model compounds with the two different laccases were compared, the redox-potential difference between laccases T1 copper and the lignin model compound (Delta E) was not the only property that determined the oxidation rate. In the case of lignin model substrates, also the selectivity of a specific laccase. reflected in the k(cat)/K-m value, plays an important role. (C) 2008 Elsevier B.V. All rights reserved.
    Original languageEnglish
    JournalJournal of Molecular Catalysis B: Enzymatic
    Volume57
    Issue number1-4
    Pages (from-to)204-210
    Number of pages7
    ISSN1381-1177
    DOIs
    Publication statusPublished - 2009
    MoE publication typeA1 Journal article-refereed

    Fields of Science

    • Laccase
    • Lignin
    • Oxidation
    • Cyclic voltammetry
    • Docking
    • BETA-GUAIACYL ETHER
    • N-HYDROXY COMPOUNDS
    • CRYSTAL-STRUCTURE
    • MELANOCARPUS-ALBOMYCES
    • REDOX POTENTIALS
    • MIXED-SOLVENTS
    • COPPER SITE
    • PHENOLS
    • SPECIFICITY
    • VERSICOLOR
    • 118 Biological sciences
    • 220 Industrial biotechnology
    • 116 Chemical sciences

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