The first known virus isolates from Antarctic sea ice have complex infection patterns

Anne-Mari Luhtanen, Eeva Eronen-Rasimus, Hanna Maarit Oksanen, Jean-Louis Tison, Bruno Delille, Gerhard S Dieckmann, Janne-Markus Rintala, Dennis Henry Bamford

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Viruses are recognized as important actors in ocean ecology and biogeochemical cycles, but many details are not yet understood. We participated in a winter expedition to the Weddell Sea, Antarctica, to isolate viruses and to measure virus-like particle abundance (flow cytometry) in sea ice. We isolated 59 bacterial strains and the first four Antarctic sea-ice viruses known (PANV1, PANV2, OANV1 and OANV2), which grow in bacterial hosts belonging to the typical sea-ice genera Paraglaciecola and Octadecabacter. The viruses were specific for bacteria at the strain level, although OANV1 was able to infect strains from two different classes. Both PANV1 and PANV2 infected 11/15 isolated Paraglaciecola strains that had almost identical 16S rRNA gene sequences, but the plating efficiencies differed among the strains, whereas OANV1 infected 3/7 Octadecabacter and 1/15 Paraglaciecola strains and OANV2 1/7 Octadecabacter strains. All the phages were cold-active and able to infect their original host at 0 degrees C and 4 degrees C, but not at higher temperatures. The results showed that virus-host interactions can be very complex and that the viral community can also be dynamic in the winter-sea ice.

Original languageEnglish
Article number028
JournalFEMS Microbiology Ecology
Volume94
Issue number4
Number of pages15
ISSN0168-6496
DOIs
Publication statusPublished - Apr 2018
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 1183 Plant biology, microbiology, virology
  • 1181 Ecology, evolutionary biology
  • virus-host interactions
  • virus isolation
  • sea ice microbiology
  • VLP in sea ice
  • BACTERIAL-GROWTH RATE
  • PHAGE-HOST SYSTEMS
  • MARINE-BACTERIA
  • SOUTHERN-OCEAN
  • PACK ICE
  • PSYCHROPHILIC BACTERIA
  • PHYLOGENETIC ANALYSES
  • PROKARYOTIC VIRUSES
  • VIRAL ABUNDANCE
  • WEDDELL SEA

Cite this

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title = "The first known virus isolates from Antarctic sea ice have complex infection patterns",
abstract = "Viruses are recognized as important actors in ocean ecology and biogeochemical cycles, but many details are not yet understood. We participated in a winter expedition to the Weddell Sea, Antarctica, to isolate viruses and to measure virus-like particle abundance (flow cytometry) in sea ice. We isolated 59 bacterial strains and the first four Antarctic sea-ice viruses known (PANV1, PANV2, OANV1 and OANV2), which grow in bacterial hosts belonging to the typical sea-ice genera Paraglaciecola and Octadecabacter. The viruses were specific for bacteria at the strain level, although OANV1 was able to infect strains from two different classes. Both PANV1 and PANV2 infected 11/15 isolated Paraglaciecola strains that had almost identical 16S rRNA gene sequences, but the plating efficiencies differed among the strains, whereas OANV1 infected 3/7 Octadecabacter and 1/15 Paraglaciecola strains and OANV2 1/7 Octadecabacter strains. All the phages were cold-active and able to infect their original host at 0 degrees C and 4 degrees C, but not at higher temperatures. The results showed that virus-host interactions can be very complex and that the viral community can also be dynamic in the winter-sea ice.",
keywords = "1183 Plant biology, microbiology, virology, 1181 Ecology, evolutionary biology, virus-host interactions, virus isolation, sea ice microbiology, VLP in sea ice, BACTERIAL-GROWTH RATE, PHAGE-HOST SYSTEMS, MARINE-BACTERIA, SOUTHERN-OCEAN, PACK ICE, PSYCHROPHILIC BACTERIA, PHYLOGENETIC ANALYSES, PROKARYOTIC VIRUSES, VIRAL ABUNDANCE, WEDDELL SEA",
author = "Anne-Mari Luhtanen and Eeva Eronen-Rasimus and Oksanen, {Hanna Maarit} and Jean-Louis Tison and Bruno Delille and Dieckmann, {Gerhard S} and Janne-Markus Rintala and Bamford, {Dennis Henry}",
year = "2018",
month = "4",
doi = "10.1093/femsec/fiy028",
language = "English",
volume = "94",
journal = "FEMS Microbiology Ecology",
issn = "0168-6496",
publisher = "Oxford University Press",
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}

The first known virus isolates from Antarctic sea ice have complex infection patterns. / Luhtanen, Anne-Mari; Eronen-Rasimus, Eeva; Oksanen, Hanna Maarit; Tison, Jean-Louis; Delille, Bruno; Dieckmann, Gerhard S; Rintala, Janne-Markus; Bamford, Dennis Henry.

In: FEMS Microbiology Ecology, Vol. 94, No. 4, 028, 04.2018.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - The first known virus isolates from Antarctic sea ice have complex infection patterns

AU - Luhtanen, Anne-Mari

AU - Eronen-Rasimus, Eeva

AU - Oksanen, Hanna Maarit

AU - Tison, Jean-Louis

AU - Delille, Bruno

AU - Dieckmann, Gerhard S

AU - Rintala, Janne-Markus

AU - Bamford, Dennis Henry

PY - 2018/4

Y1 - 2018/4

N2 - Viruses are recognized as important actors in ocean ecology and biogeochemical cycles, but many details are not yet understood. We participated in a winter expedition to the Weddell Sea, Antarctica, to isolate viruses and to measure virus-like particle abundance (flow cytometry) in sea ice. We isolated 59 bacterial strains and the first four Antarctic sea-ice viruses known (PANV1, PANV2, OANV1 and OANV2), which grow in bacterial hosts belonging to the typical sea-ice genera Paraglaciecola and Octadecabacter. The viruses were specific for bacteria at the strain level, although OANV1 was able to infect strains from two different classes. Both PANV1 and PANV2 infected 11/15 isolated Paraglaciecola strains that had almost identical 16S rRNA gene sequences, but the plating efficiencies differed among the strains, whereas OANV1 infected 3/7 Octadecabacter and 1/15 Paraglaciecola strains and OANV2 1/7 Octadecabacter strains. All the phages were cold-active and able to infect their original host at 0 degrees C and 4 degrees C, but not at higher temperatures. The results showed that virus-host interactions can be very complex and that the viral community can also be dynamic in the winter-sea ice.

AB - Viruses are recognized as important actors in ocean ecology and biogeochemical cycles, but many details are not yet understood. We participated in a winter expedition to the Weddell Sea, Antarctica, to isolate viruses and to measure virus-like particle abundance (flow cytometry) in sea ice. We isolated 59 bacterial strains and the first four Antarctic sea-ice viruses known (PANV1, PANV2, OANV1 and OANV2), which grow in bacterial hosts belonging to the typical sea-ice genera Paraglaciecola and Octadecabacter. The viruses were specific for bacteria at the strain level, although OANV1 was able to infect strains from two different classes. Both PANV1 and PANV2 infected 11/15 isolated Paraglaciecola strains that had almost identical 16S rRNA gene sequences, but the plating efficiencies differed among the strains, whereas OANV1 infected 3/7 Octadecabacter and 1/15 Paraglaciecola strains and OANV2 1/7 Octadecabacter strains. All the phages were cold-active and able to infect their original host at 0 degrees C and 4 degrees C, but not at higher temperatures. The results showed that virus-host interactions can be very complex and that the viral community can also be dynamic in the winter-sea ice.

KW - 1183 Plant biology, microbiology, virology

KW - 1181 Ecology, evolutionary biology

KW - virus-host interactions

KW - virus isolation

KW - sea ice microbiology

KW - VLP in sea ice

KW - BACTERIAL-GROWTH RATE

KW - PHAGE-HOST SYSTEMS

KW - MARINE-BACTERIA

KW - SOUTHERN-OCEAN

KW - PACK ICE

KW - PSYCHROPHILIC BACTERIA

KW - PHYLOGENETIC ANALYSES

KW - PROKARYOTIC VIRUSES

KW - VIRAL ABUNDANCE

KW - WEDDELL SEA

U2 - 10.1093/femsec/fiy028

DO - 10.1093/femsec/fiy028

M3 - Article

VL - 94

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

SN - 0168-6496

IS - 4

M1 - 028

ER -