The layered fold of the TSR domain of P. falciparum TRAP contains a heparin binding site

Helena Tossavainen, Tero Pihlajamaa, Toni K Huttunen, Erkki Raulo, Heikki Rauvala, Perttu Permi, Ilkka Kilpeläinen

    Research output: Contribution to journalArticleScientificpeer-review

    Abstract

    Thrombospondin-related anonymous protein, TRAP, has a critical role in the hepatocyte invasion step of Plasmodium sporozoites, the transmissible form of the parasite causing malaria. The extracellular domains of this sporozoite surface protein interact with hepatocyte surface receptors whereas its intracellular domain acts as a link to the sporozoite actomyosin motor system. Liver heparan sulfate proteoglycans have been identified as potential ligands for TRAP. Proteoglycan binding has been associated with the A- and TSR domains of TRAP. We present the solution NMR structure of the TSR domain of TRAP and a chemical shift mapping study of its heparin binding epitope. The domain has an elongated structure stabilized by an array of tryptophan and arginine residues as well as disulfide bonds. The fold is very similar to those of thrombospondin type-1 (TSP-1) and F-spondin TSRs. The heparin binding site of TRAP-TSR is located in the N-terminal half of the structure, the layered side chains forming an integral part of the site. The smallest heparin fragment capable of binding to TRAP-TSR is a tetrasaccharide.
    Original languageEnglish
    JournalProtein Science
    Volume15
    Issue number7
    Pages (from-to)1760-1768
    Number of pages9
    ISSN0961-8368
    DOIs
    Publication statusPublished - 2006
    MoE publication typeA1 Journal article-refereed

    Fields of Science

    • 311 Basic medicine
    • 118 Biological sciences
    • 515 Psychology

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