The plasticity of mesenchymal stem cells in regulating surface HLA-I

Yafei Wang, Jiayun Huang, Lin Gong, Dongsheng Yu, Chenrui An, Varitsara Bunpetch, Jun Dai, He Huang, Xiaohui Zou, Hongwei Ouyang, Hua Liu

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Summary A low surface expression level of human leukocyte antigen class I(HLA-I) ensures the mesenchymal stem cells’(MSCs) escape from the allogeneic recipients’ immunological surveillance. Here, we discovered that both transcriptional and synthesis levels of HLA-I in MSCs increased continuously after IFN-γ treatment, while interestingly, their surface HLA-I expression was downregulated after reaching an HLA-I surface expression peak. Microarray data indicated the post-transcriptional process plays an important role in downregulation of surface HLA-I. Further studies identified that IFN-γ-treated MSCs accelerated HLA-I endocytosis through a Clathrin–independent Dynamin-dependent endocytosis pathway. Furthermore, the cells which have self-downregulated surface HLA-I expression elicit a weaker immune response than they previously could. Thus, uncovering the plasticity of MSCs in the regulation of HLA-I surface expression would reveal insights into the membrane-transportation events leading to the maintenance of low surface HLA-I expression, providing more evidence for selecting and optimizing low immunogenic MSCs to improve the therapeutic efficiency.
Original languageEnglish
JournaliScience
ISSN2589-0042
DOIs
Publication statusPublished - 11 Apr 2019
MoE publication typeA1 Journal article-refereed

Cite this

Wang, Yafei ; Huang, Jiayun ; Gong, Lin ; Yu, Dongsheng ; An, Chenrui ; Bunpetch, Varitsara ; Dai, Jun ; Huang, He ; Zou, Xiaohui ; Ouyang, Hongwei ; Liu, Hua. / The plasticity of mesenchymal stem cells in regulating surface HLA-I. In: iScience. 2019.
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title = "The plasticity of mesenchymal stem cells in regulating surface HLA-I",
abstract = "Summary A low surface expression level of human leukocyte antigen class I(HLA-I) ensures the mesenchymal stem cells’(MSCs) escape from the allogeneic recipients’ immunological surveillance. Here, we discovered that both transcriptional and synthesis levels of HLA-I in MSCs increased continuously after IFN-γ treatment, while interestingly, their surface HLA-I expression was downregulated after reaching an HLA-I surface expression peak. Microarray data indicated the post-transcriptional process plays an important role in downregulation of surface HLA-I. Further studies identified that IFN-γ-treated MSCs accelerated HLA-I endocytosis through a Clathrin–independent Dynamin-dependent endocytosis pathway. Furthermore, the cells which have self-downregulated surface HLA-I expression elicit a weaker immune response than they previously could. Thus, uncovering the plasticity of MSCs in the regulation of HLA-I surface expression would reveal insights into the membrane-transportation events leading to the maintenance of low surface HLA-I expression, providing more evidence for selecting and optimizing low immunogenic MSCs to improve the therapeutic efficiency.",
author = "Yafei Wang and Jiayun Huang and Lin Gong and Dongsheng Yu and Chenrui An and Varitsara Bunpetch and Jun Dai and He Huang and Xiaohui Zou and Hongwei Ouyang and Hua Liu",
year = "2019",
month = "4",
day = "11",
doi = "10.1016/j.isci.2019.04.011",
language = "English",
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Wang, Y, Huang, J, Gong, L, Yu, D, An, C, Bunpetch, V, Dai, J, Huang, H, Zou, X, Ouyang, H & Liu, H 2019, 'The plasticity of mesenchymal stem cells in regulating surface HLA-I' iScience. https://doi.org/10.1016/j.isci.2019.04.011

The plasticity of mesenchymal stem cells in regulating surface HLA-I. / Wang, Yafei; Huang, Jiayun; Gong, Lin; Yu, Dongsheng; An, Chenrui; Bunpetch, Varitsara; Dai, Jun; Huang, He; Zou, Xiaohui; Ouyang, Hongwei; Liu, Hua.

In: iScience, 11.04.2019.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - The plasticity of mesenchymal stem cells in regulating surface HLA-I

AU - Wang, Yafei

AU - Huang, Jiayun

AU - Gong, Lin

AU - Yu, Dongsheng

AU - An, Chenrui

AU - Bunpetch, Varitsara

AU - Dai, Jun

AU - Huang, He

AU - Zou, Xiaohui

AU - Ouyang, Hongwei

AU - Liu, Hua

PY - 2019/4/11

Y1 - 2019/4/11

N2 - Summary A low surface expression level of human leukocyte antigen class I(HLA-I) ensures the mesenchymal stem cells’(MSCs) escape from the allogeneic recipients’ immunological surveillance. Here, we discovered that both transcriptional and synthesis levels of HLA-I in MSCs increased continuously after IFN-γ treatment, while interestingly, their surface HLA-I expression was downregulated after reaching an HLA-I surface expression peak. Microarray data indicated the post-transcriptional process plays an important role in downregulation of surface HLA-I. Further studies identified that IFN-γ-treated MSCs accelerated HLA-I endocytosis through a Clathrin–independent Dynamin-dependent endocytosis pathway. Furthermore, the cells which have self-downregulated surface HLA-I expression elicit a weaker immune response than they previously could. Thus, uncovering the plasticity of MSCs in the regulation of HLA-I surface expression would reveal insights into the membrane-transportation events leading to the maintenance of low surface HLA-I expression, providing more evidence for selecting and optimizing low immunogenic MSCs to improve the therapeutic efficiency.

AB - Summary A low surface expression level of human leukocyte antigen class I(HLA-I) ensures the mesenchymal stem cells’(MSCs) escape from the allogeneic recipients’ immunological surveillance. Here, we discovered that both transcriptional and synthesis levels of HLA-I in MSCs increased continuously after IFN-γ treatment, while interestingly, their surface HLA-I expression was downregulated after reaching an HLA-I surface expression peak. Microarray data indicated the post-transcriptional process plays an important role in downregulation of surface HLA-I. Further studies identified that IFN-γ-treated MSCs accelerated HLA-I endocytosis through a Clathrin–independent Dynamin-dependent endocytosis pathway. Furthermore, the cells which have self-downregulated surface HLA-I expression elicit a weaker immune response than they previously could. Thus, uncovering the plasticity of MSCs in the regulation of HLA-I surface expression would reveal insights into the membrane-transportation events leading to the maintenance of low surface HLA-I expression, providing more evidence for selecting and optimizing low immunogenic MSCs to improve the therapeutic efficiency.

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M3 - Article

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JF - iScience

SN - 2589-0042

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