The ribose 5-phosphate isomerase-encoding gene is located immediately downstream from that encoding murine immunoglobulin k

The immunoglobulin kappa locus (Igx) is active only in the B-lymphocyte cell lineage. By exon-trapping we found a gene situated downstream from the murine Igx locus. This gene encodes a protein with 53% sequence identity to the ribose 5-phosphate isomerase A (RPI-A) of Escherichia coli and is therefore likely to be the murine homologue (mRPI) of this enzyme. We confirmed this assumption by showing that a glutathione S-transferase (GST)::mRPI fusion protein has enzymatic activity and that an anti-mRPI antibody detects a protein of the predicted mass of RPI (33 kDa). Cloning and sequencing of the human counterpart show that the RPI gene is evolutionarily conserved. The expression of mRPI is not influenced by the rearrangement status of the Igx locus in B cells and mRPI is expressed in all tissues. We thus show that two genes with very different expression patterns, a housekeeping gene and a gene expressed in a tissue- specific manner, can be located on a chromosome in close proximity to each other.